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The role of lipopolysaccharide and Shiga-like toxin in a mouse model of Escherichia coli O157:H7 infection

Karpman, D LU ; Connell, H LU ; Svensson, Majlis LU ; Scheutz, F; Alm, Per LU and Svanborg, C LU (1997) In Journal of Infectious Diseases 175(3). p.20-611
Abstract

The role of lipopolysaccharide (LPS) and Shiga-like toxin (SLT) in the pathogenesis of hemolytic uremic syndrome (HUS) was studied in a mouse model. Mice inoculated intragastrically with Escherichia coli O157:H7 developed gastrointestinal, neurologic, and systemic symptoms, necrotic foci in the colon, glomerular and tubular histopathology, and fragmented erythrocytes. LPS-responder (C3H/HeN) mice developed a combination of neurologic and systemic symptoms, whereas LPS-nonresponder (C3H/HeJ) mice had a biphasic course of disease, first developing systemic symptoms and later severe neurologic symptoms. Mice inoculated with SLT-II-positive strains developed severe neurotoxic symptoms and a higher frequency of systemic symptoms and... (More)

The role of lipopolysaccharide (LPS) and Shiga-like toxin (SLT) in the pathogenesis of hemolytic uremic syndrome (HUS) was studied in a mouse model. Mice inoculated intragastrically with Escherichia coli O157:H7 developed gastrointestinal, neurologic, and systemic symptoms, necrotic foci in the colon, glomerular and tubular histopathology, and fragmented erythrocytes. LPS-responder (C3H/HeN) mice developed a combination of neurologic and systemic symptoms, whereas LPS-nonresponder (C3H/HeJ) mice had a biphasic course of disease, first developing systemic symptoms and later severe neurologic symptoms. Mice inoculated with SLT-II-positive strains developed severe neurotoxic symptoms and a higher frequency of systemic symptoms and glomerular pathology compared with SLT-II-negative strains. Anti-SLT-II antibodies protected against these symptoms and pathology. These results demonstrate that this model could be used to study aspects of human HUS and that both LPS and SLT are important for disease development.

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keywords
Animals, Bacterial Toxins, Disease Models, Animal, Escherichia coli Infections, Escherichia coli O157, Female, Hemolysis, Hemolytic-Uremic Syndrome, Lipopolysaccharides, Male, Mice, Mice, Inbred C3H, Shiga Toxin 2, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Infectious Diseases
volume
175
issue
3
pages
10 pages
publisher
Oxford University Press
external identifiers
  • scopus:16944365096
ISSN
0022-1899
DOI
10.1093/infdis/175.3.611
language
English
LU publication?
yes
id
8181168a-780d-48b7-8930-b85f24745403
date added to LUP
2017-02-08 16:04:26
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2017-02-12 04:39:30
@article{8181168a-780d-48b7-8930-b85f24745403,
  abstract     = {<p>The role of lipopolysaccharide (LPS) and Shiga-like toxin (SLT) in the pathogenesis of hemolytic uremic syndrome (HUS) was studied in a mouse model. Mice inoculated intragastrically with Escherichia coli O157:H7 developed gastrointestinal, neurologic, and systemic symptoms, necrotic foci in the colon, glomerular and tubular histopathology, and fragmented erythrocytes. LPS-responder (C3H/HeN) mice developed a combination of neurologic and systemic symptoms, whereas LPS-nonresponder (C3H/HeJ) mice had a biphasic course of disease, first developing systemic symptoms and later severe neurologic symptoms. Mice inoculated with SLT-II-positive strains developed severe neurotoxic symptoms and a higher frequency of systemic symptoms and glomerular pathology compared with SLT-II-negative strains. Anti-SLT-II antibodies protected against these symptoms and pathology. These results demonstrate that this model could be used to study aspects of human HUS and that both LPS and SLT are important for disease development.</p>},
  author       = {Karpman, D and Connell, H and Svensson, Majlis and Scheutz, F and Alm, Per and Svanborg, C},
  issn         = {0022-1899},
  keyword      = {Animals,Bacterial Toxins,Disease Models, Animal,Escherichia coli Infections,Escherichia coli O157,Female,Hemolysis,Hemolytic-Uremic Syndrome,Lipopolysaccharides,Male,Mice,Mice, Inbred C3H,Shiga Toxin 2,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {20--611},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {The role of lipopolysaccharide and Shiga-like toxin in a mouse model of Escherichia coli O157:H7 infection},
  url          = {http://dx.doi.org/10.1093/infdis/175.3.611},
  volume       = {175},
  year         = {1997},
}