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Increased platelet activation occurs in cystic fibrosis patients and correlates to clinical status

Lindberg, Ulrika LU ; Svensson, Lisbeth LU ; Hellmark, Thomas LU ; Segelmark, Mårten and Shannon, Oonagh LU (2018) In Thrombosis Research 162. p.32-37
Abstract

Cystic fibrosis (CF) is an inflammatory lung disease. Platelets have an emerging role in inflammation, however previous studies of platelet activation in CF have generated conflicting results. In this study, we determined platelet function in CF patients and correlated platelet activation to establish clinical and laboratory parameters. Twenty-two patients, aged 20.7 to 54.4 (mean 34.0, SD 9.45) years and with a mean FEV1%pred (forced expiratory volume in one second, % of predicted) of 72 (SD 21.4, range 32–110) were recruited. A combination of platelet assays was used: platelet aggregation, platelet activation and platelet-leukocyte complex formation. Platelets from CF patients exhibited significantly increased aggregation when... (More)

Cystic fibrosis (CF) is an inflammatory lung disease. Platelets have an emerging role in inflammation, however previous studies of platelet activation in CF have generated conflicting results. In this study, we determined platelet function in CF patients and correlated platelet activation to establish clinical and laboratory parameters. Twenty-two patients, aged 20.7 to 54.4 (mean 34.0, SD 9.45) years and with a mean FEV1%pred (forced expiratory volume in one second, % of predicted) of 72 (SD 21.4, range 32–110) were recruited. A combination of platelet assays was used: platelet aggregation, platelet activation and platelet-leukocyte complex formation. Platelets from CF patients exhibited significantly increased aggregation when stimulated ex-vivo, a tendency towards increased platelet upregulation of CD62P, but no increase of GPIIb/IIIa activation (PAC-1). Platelet-monocyte complex (PMC) formation was significantly increased in CF patients compared to controls, while platelet-neutrophil complex formation was not. In the CF group, platelet aggregation correlates with levels of anti-neutrophil cytoplasmic antibodies (ANCA) with specificity for bactericidal/permeability-increasing protein (BPI), BPI-ANCA (r = 0.56). The formation of PMCs correlates with lung function decline (1-FEV1%), CRP and BPI-ANCA (r = 0.61, 0.55, 0.5). We therefore confirm the presence of increased platelet activation in CF patients, and determine that further evaluation of platelet activation in relation to prognostic factors in CF is warranted.

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type
Contribution to journal
publication status
published
subject
keywords
Anti-neutrophil cytoplasmic antibodies, BPI-ANCA, Cystic fibrosis, Platelet activation, Platelet aggregation, Platelet-leukocyte complex formation
in
Thrombosis Research
volume
162
pages
6 pages
publisher
Elsevier Ltd
external identifiers
  • scopus:85038809189
ISSN
0049-3848
DOI
10.1016/j.thromres.2017.12.012
language
English
LU publication?
yes
id
82834a22-b0cb-4721-860b-7319a1e73fab
date added to LUP
2018-01-02 12:00:22
date last changed
2018-01-20 10:07:43
@article{82834a22-b0cb-4721-860b-7319a1e73fab,
  abstract     = {<p>Cystic fibrosis (CF) is an inflammatory lung disease. Platelets have an emerging role in inflammation, however previous studies of platelet activation in CF have generated conflicting results. In this study, we determined platelet function in CF patients and correlated platelet activation to establish clinical and laboratory parameters. Twenty-two patients, aged 20.7 to 54.4 (mean 34.0, SD 9.45) years and with a mean FEV1%pred (forced expiratory volume in one second, % of predicted) of 72 (SD 21.4, range 32–110) were recruited. A combination of platelet assays was used: platelet aggregation, platelet activation and platelet-leukocyte complex formation. Platelets from CF patients exhibited significantly increased aggregation when stimulated ex-vivo, a tendency towards increased platelet upregulation of CD62P, but no increase of GPIIb/IIIa activation (PAC-1). Platelet-monocyte complex (PMC) formation was significantly increased in CF patients compared to controls, while platelet-neutrophil complex formation was not. In the CF group, platelet aggregation correlates with levels of anti-neutrophil cytoplasmic antibodies (ANCA) with specificity for bactericidal/permeability-increasing protein (BPI), BPI-ANCA (r = 0.56). The formation of PMCs correlates with lung function decline (1-FEV1%), CRP and BPI-ANCA (r = 0.61, 0.55, 0.5). We therefore confirm the presence of increased platelet activation in CF patients, and determine that further evaluation of platelet activation in relation to prognostic factors in CF is warranted.</p>},
  author       = {Lindberg, Ulrika and Svensson, Lisbeth and Hellmark, Thomas and Segelmark, Mårten and Shannon, Oonagh},
  issn         = {0049-3848},
  keyword      = {Anti-neutrophil cytoplasmic antibodies,BPI-ANCA,Cystic fibrosis,Platelet activation,Platelet aggregation,Platelet-leukocyte complex formation},
  language     = {eng},
  month        = {02},
  pages        = {32--37},
  publisher    = {Elsevier Ltd},
  series       = {Thrombosis Research},
  title        = {Increased platelet activation occurs in cystic fibrosis patients and correlates to clinical status},
  url          = {http://dx.doi.org/10.1016/j.thromres.2017.12.012},
  volume       = {162},
  year         = {2018},
}