Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
(2017) In Scientific Data 4.- Abstract
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ∼600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than... (More)
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ∼600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
(Less)
- author
- Reddy, Hemanth K.N.
; Yoon, Chun Hong
; Aquila, Andrew
; Awel, Salah
; Ayyer, Kartik
; Barty, Anton
; Berntsen, Peter
; Bielecki, Johan
; Bobkov, Sergey
; Bucher, Maximilian
; Carini, Gabriella A.
; Carron, Sebastian
; Chapman, Henry
; Daurer, Benedikt
; Demirci, Hasan
; Ekeberg, Tomas
; Fromme, Petra
; Hajdu, Janos
; Hanke, Max Felix
; Hart, Philip
; Hogue, Brenda G.
; Hosseinizadeh, Ahmad
; Kim, Yoonhee
; Kirian, Richard A.
; Kurta, Ruslan P.
; Larsson, Daniel S.D.
; Duane Loh, N.
; Maia, Filipe R.N.C.
; Mancuso, Adrian P.
; Mühlig, Kerstin
; Munke, Anna
LU
; Nam, Daewoong
; Nettelblad, Carl
; Ourmazd, Abbas
; Rose, Max
; Schwander, Peter
; Seibert, Marvin
; Sellberg, Jonas A.
; Song, Changyong
; Spence, John C.H.
; Svenda, Martin
; van der Schot, Gijs
; Vartanyants, Ivan A.
; Williams, Garth J.
and Lourdu Xavier, P.
(Less) - publishing date
- 2017-06-27
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- coherent diffractive imaging, single-particle imaging, virus, PR772, phage, XFEL, free-electron laser, X-ray free-electron laser (XFEL)
- in
- Scientific Data
- volume
- 4
- article number
- 170079
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:28654088
- scopus:85021625011
- ISSN
- 2052-4463
- DOI
- 10.1038/sdata.2017.79
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2017 The Author(s).
- id
- 83327004-f035-4122-b111-91e866171a9d
- date added to LUP
- 2025-04-21 20:57:03
- date last changed
- 2025-04-30 03:34:10
@article{83327004-f035-4122-b111-91e866171a9d,
abstract = {{<p>Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ∼600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.</p>}},
author = {{Reddy, Hemanth K.N. and Yoon, Chun Hong and Aquila, Andrew and Awel, Salah and Ayyer, Kartik and Barty, Anton and Berntsen, Peter and Bielecki, Johan and Bobkov, Sergey and Bucher, Maximilian and Carini, Gabriella A. and Carron, Sebastian and Chapman, Henry and Daurer, Benedikt and Demirci, Hasan and Ekeberg, Tomas and Fromme, Petra and Hajdu, Janos and Hanke, Max Felix and Hart, Philip and Hogue, Brenda G. and Hosseinizadeh, Ahmad and Kim, Yoonhee and Kirian, Richard A. and Kurta, Ruslan P. and Larsson, Daniel S.D. and Duane Loh, N. and Maia, Filipe R.N.C. and Mancuso, Adrian P. and Mühlig, Kerstin and Munke, Anna and Nam, Daewoong and Nettelblad, Carl and Ourmazd, Abbas and Rose, Max and Schwander, Peter and Seibert, Marvin and Sellberg, Jonas A. and Song, Changyong and Spence, John C.H. and Svenda, Martin and van der Schot, Gijs and Vartanyants, Ivan A. and Williams, Garth J. and Lourdu Xavier, P.}},
issn = {{2052-4463}},
keywords = {{coherent diffractive imaging; single-particle imaging; virus; PR772; phage; XFEL; free-electron laser; X-ray free-electron laser (XFEL)}},
language = {{eng}},
month = {{06}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Data}},
title = {{Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source}},
url = {{http://dx.doi.org/10.1038/sdata.2017.79}},
doi = {{10.1038/sdata.2017.79}},
volume = {{4}},
year = {{2017}},
}