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Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

Li, Haoyuan ; Nazari, Reza ; Abbey, Brian ; Alvarez, Roberto ; Aquila, Andrew ; Ayyer, Kartik ; Barty, Anton ; Berntsen, Peter ; Bielecki, Johan and Pietrini, Alberto , et al. (2020) In Scientific Data 7(1).
Abstract

Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of... (More)

Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
XFEL, X-ray free-electron laser (XFEL), coherent diffractive imaging, single-particle imaging, virus, PR772, phage
in
Scientific Data
volume
7
issue
1
article number
404
publisher
Nature Publishing Group
external identifiers
  • pmid:33214568
  • scopus:85096341423
ISSN
2052-4463
DOI
10.1038/s41597-020-00745-2
language
English
LU publication?
no
additional info
Publisher Copyright: © 2020, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.
id
b654a2c6-7344-4465-83fe-82e6bbabe3ba
date added to LUP
2025-04-21 20:51:02
date last changed
2025-04-24 03:35:31
@article{b654a2c6-7344-4465-83fe-82e6bbabe3ba,
  abstract     = {{<p>Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.</p>}},
  author       = {{Li, Haoyuan and Nazari, Reza and Abbey, Brian and Alvarez, Roberto and Aquila, Andrew and Ayyer, Kartik and Barty, Anton and Berntsen, Peter and Bielecki, Johan and Pietrini, Alberto and Bucher, Maximilian and Carini, Gabriella and Chapman, Henry N. and Contreras, Alice and Daurer, Benedikt J. and DeMirci, Hasan and Flűckiger, Leonie and Frank, Matthias and Hajdu, Janos and Hantke, Max F. and Hogue, Brenda G. and Hosseinizadeh, Ahmad and Hunter, Mark S. and Jönsson, H. Olof and Kirian, Richard A. and Kurta, Ruslan P. and Loh, Duane and Maia, Filipe R.N.C. and Mancuso, Adrian P. and Morgan, Andrew J. and McFadden, Matthew and Muehlig, Kerstin and Munke, Anna and Reddy, Hemanth Kumar Narayana and Nettelblad, Carl and Ourmazd, Abbas and Rose, Max and Schwander, Peter and Marvin Seibert, M. and Sellberg, Jonas A. and Sierra, Raymond G. and Sun, Zhibin and Svenda, Martin and Vartanyants, Ivan A. and Walter, Peter and Westphal, Daniel and Williams, Garth and Xavier, P. Lourdu and Yoon, Chun Hong and Zaare, Sahba}},
  issn         = {{2052-4463}},
  keywords     = {{XFEL; X-ray free-electron laser (XFEL); coherent diffractive imaging; single-particle imaging; virus; PR772; phage}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Data}},
  title        = {{Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source}},
  url          = {{http://dx.doi.org/10.1038/s41597-020-00745-2}},
  doi          = {{10.1038/s41597-020-00745-2}},
  volume       = {{7}},
  year         = {{2020}},
}