Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses

Kurta, Ruslan P. ; Donatelli, Jeffrey J. ; Yoon, Chun Hong ; Berntsen, Peter ; Bielecki, Johan ; Daurer, Benedikt J. ; Demirci, Hasan ; Fromme, Petra ; Hantke, Max Felix and Maia, Filipe R.N.C. , et al. (2017) In Physical Review Letters 119(15).
Abstract

We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry.... (More)

We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; and (Less)
publishing date
type
Contribution to journal
publication status
published
subject
keywords
coherent diffractive imaging, single-particle imaging, phage, PR772, virus, XFEL, free-electron laser, X-ray free-electron laser (XFEL)
in
Physical Review Letters
volume
119
issue
15
article number
158102
publisher
American Physical Society
external identifiers
  • pmid:29077445
  • scopus:85031315313
ISSN
0031-9007
DOI
10.1103/PhysRevLett.119.158102
language
English
LU publication?
no
additional info
Publisher Copyright: © 2017 American Physical Society.
id
e18c58e7-4f0b-4e83-ade5-0ec96c07ddb5
date added to LUP
2025-04-21 20:55:54
date last changed
2025-06-17 01:11:42
@article{e18c58e7-4f0b-4e83-ade5-0ec96c07ddb5,
  abstract     = {{<p>We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.</p>}},
  author       = {{Kurta, Ruslan P. and Donatelli, Jeffrey J. and Yoon, Chun Hong and Berntsen, Peter and Bielecki, Johan and Daurer, Benedikt J. and Demirci, Hasan and Fromme, Petra and Hantke, Max Felix and Maia, Filipe R.N.C. and Munke, Anna and Nettelblad, Carl and Pande, Kanupriya and Reddy, Hemanth K.N. and Sellberg, Jonas A. and Sierra, Raymond G. and Svenda, Martin and van der Schot, Gijs and Vartanyants, Ivan A. and Williams, Garth J. and Xavier, P. Lourdu and Aquila, Andrew and Zwart, Peter H. and Mancuso, Adrian P.}},
  issn         = {{0031-9007}},
  keywords     = {{coherent diffractive imaging; single-particle imaging; phage; PR772; virus; XFEL; free-electron laser; X-ray free-electron laser (XFEL)}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{15}},
  publisher    = {{American Physical Society}},
  series       = {{Physical Review Letters}},
  title        = {{Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses}},
  url          = {{http://dx.doi.org/10.1103/PhysRevLett.119.158102}},
  doi          = {{10.1103/PhysRevLett.119.158102}},
  volume       = {{119}},
  year         = {{2017}},
}