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Label-FreeHigh-ResolutionPhotothermalOpticalInfraredSpectroscopyfor Spatiotemporal Chemica lAnalysis in Fresh, Hydrated Living Tissues and Embryos

Gvazava, Nika LU ; Konings, Sabine LU orcid ; Cepeda-Prado, Efrain LU orcid ; Skoryk, Valeriia LU ; Harrison Umeano, Chimezie LU ; Dong, Jiao LU orcid ; Augusto Silva, Iran LU orcid ; Rylander Ottosson, Daniella LU ; Leigh, Nicholas LU orcid and Wagner, Darcy LU orcid , et al. (2023) In Journal of the American Chemical Society
Abstract
Label-free chemical imaging of living and functioning systems is the holy grail of biochemical research. However, existing techniques often require extensive sample preparation to remove interfering molecules such as water, rendering many molecular imaging techniques unsuitable for in situ structural studies. Here, we examined freshly extracted tissue biopsies and living small vertebrates at submicrometer resolution using optical photothermal infrared (O-PTIR) microspectroscopy and demonstrated the following major advances: (1) O-PTIR can be used for submicrometer structural analysis of unprocessed, fully hydrated tissue biopsies extracted from diverse organs, including living brain and lung tissues. (2) O-PTIR imaging can be performed on... (More)
Label-free chemical imaging of living and functioning systems is the holy grail of biochemical research. However, existing techniques often require extensive sample preparation to remove interfering molecules such as water, rendering many molecular imaging techniques unsuitable for in situ structural studies. Here, we examined freshly extracted tissue biopsies and living small vertebrates at submicrometer resolution using optical photothermal infrared (O-PTIR) microspectroscopy and demonstrated the following major advances: (1) O-PTIR can be used for submicrometer structural analysis of unprocessed, fully hydrated tissue biopsies extracted from diverse organs, including living brain and lung tissues. (2) O-PTIR imaging can be performed on living organisms, such as salamander embryos, without compromising their further development. (3) Using O-PTIR, we tracked the structural changes of amyloids in functioning brain tissues over time, observing the appearance of newly formed amyloids for the first time. (4) Amyloid structures appeared altered following standard fixation and dehydration procedures. Thus, we demonstrate that O-PTIR enables time-resolved submicrometer in situ investigation of chemical and structural changes in diverse biomolecules in their native conditions, representing a technological breakthrough for in situ molecular imaging of biological samples. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the American Chemical Society
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85178245571
ISSN
1520-5126
DOI
10.1021/jacs.3c08854
language
English
LU publication?
yes
id
83faad03-cc09-4b80-9a82-bcdc91ed2064
date added to LUP
2023-11-12 21:42:17
date last changed
2023-12-18 10:56:27
@article{83faad03-cc09-4b80-9a82-bcdc91ed2064,
  abstract     = {{Label-free chemical imaging of living and functioning systems is the holy grail of biochemical research. However, existing techniques often require extensive sample preparation to remove interfering molecules such as water, rendering many molecular imaging techniques unsuitable for in situ structural studies. Here, we examined freshly extracted tissue biopsies and living small vertebrates at submicrometer resolution using optical photothermal infrared (O-PTIR) microspectroscopy and demonstrated the following major advances: (1) O-PTIR can be used for submicrometer structural analysis of unprocessed, fully hydrated tissue biopsies extracted from diverse organs, including living brain and lung tissues. (2) O-PTIR imaging can be performed on living organisms, such as salamander embryos, without compromising their further development. (3) Using O-PTIR, we tracked the structural changes of amyloids in functioning brain tissues over time, observing the appearance of newly formed amyloids for the first time. (4) Amyloid structures appeared altered following standard fixation and dehydration procedures. Thus, we demonstrate that O-PTIR enables time-resolved submicrometer in situ investigation of chemical and structural changes in diverse biomolecules in their native conditions, representing a technological breakthrough for in situ molecular imaging of biological samples.}},
  author       = {{Gvazava, Nika and Konings, Sabine and Cepeda-Prado, Efrain and Skoryk, Valeriia and Harrison Umeano, Chimezie and Dong, Jiao and Augusto Silva, Iran and Rylander Ottosson, Daniella and Leigh, Nicholas and Wagner, Darcy and Klementieva, Oxana}},
  issn         = {{1520-5126}},
  language     = {{eng}},
  month        = {{11}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of the American Chemical Society}},
  title        = {{Label-FreeHigh-ResolutionPhotothermalOpticalInfraredSpectroscopyfor Spatiotemporal Chemica lAnalysis in Fresh, Hydrated Living Tissues and Embryos}},
  url          = {{http://dx.doi.org/10.1021/jacs.3c08854}},
  doi          = {{10.1021/jacs.3c08854}},
  year         = {{2023}},
}