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Environmental factors influencing growth of and exopolysaccharide formation by Pediococcus parvulus 2.6

Velasco, Susana LU ; Årsköld, Emma LU ; Paese, Marco LU ; Grage, Halfdan LU ; Irastorza, A.; Rådström, Peter LU and van Niel, Ed LU (2006) In International Journal of Food Microbiology 111(3). p.252-258
Abstract
Natural exopolysaccharides (EPSs) from food-grade lactic acid bacteria have potential for development and exploitation as food additives and functional food ingredients with both health and economic benefits. In this study, we have examined the physiological capacity of EPS production in Pediococcus parvulus 2.6. EPS formation by P. parvulus 2.6 was found to be linked to biomass yields, provided that glucose was not limiting. Higher biomass yields and EPS productions were obtained when cultures were pH-controlled at pH 5.2. Various compounds have been tested for their influence on growth rate and EPS formation. Of those, only glucose (up to 75 g 1(-1)), ethanol (up to 4.9%, w/v) and glycerol (up to 6.6%, w/v) had positive effects on EPS... (More)
Natural exopolysaccharides (EPSs) from food-grade lactic acid bacteria have potential for development and exploitation as food additives and functional food ingredients with both health and economic benefits. In this study, we have examined the physiological capacity of EPS production in Pediococcus parvulus 2.6. EPS formation by P. parvulus 2.6 was found to be linked to biomass yields, provided that glucose was not limiting. Higher biomass yields and EPS productions were obtained when cultures were pH-controlled at pH 5.2. Various compounds have been tested for their influence on growth rate and EPS formation. Of those, only glucose (up to 75 g 1(-1)), ethanol (up to 4.9%, w/v) and glycerol (up to 6.6%, w/v) had positive effects on EPS production. EPS production was not directly linked to growth, because its production continued in the stationary phase provided that glucose was present. According to an empirical model, the growth of R parvulus 2.6 was completely inhibited by 58.9 +/- 18.1 gl(-1) lactate. Lactate, the sole fermentation product, was suggested to affect growth by chelation of manganese. The organism grew in an apparent linear fashion due to this imposed manganese limitation. This could be overcome by increasing the manganese concentration to at least 2 mg l(-1) in the medium. The excretion of Mn2+ upon depletion of glucose indicated that maintenance of the high Mn2+ gradient over the cell membrane is an energy requiring process. EPS production was increased from 0.12 gl(-1) to 4.10 gl(-1) in an improved medium that is based on the results from this study. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
growth, Pediococcus parvulus, exopolysaccharides, manganese
in
International Journal of Food Microbiology
volume
111
issue
3
pages
252 - 258
publisher
Elsevier
external identifiers
  • wos:000241308300010
  • scopus:33748544599
ISSN
0168-1605
DOI
10.1016/j.ijfoodmicro.2006.06.008
language
English
LU publication?
yes
id
84b5faa7-a9b7-452b-9436-4902652b0add (old id 387975)
date added to LUP
2007-10-20 15:32:25
date last changed
2019-04-02 01:36:45
@article{84b5faa7-a9b7-452b-9436-4902652b0add,
  abstract     = {Natural exopolysaccharides (EPSs) from food-grade lactic acid bacteria have potential for development and exploitation as food additives and functional food ingredients with both health and economic benefits. In this study, we have examined the physiological capacity of EPS production in Pediococcus parvulus 2.6. EPS formation by P. parvulus 2.6 was found to be linked to biomass yields, provided that glucose was not limiting. Higher biomass yields and EPS productions were obtained when cultures were pH-controlled at pH 5.2. Various compounds have been tested for their influence on growth rate and EPS formation. Of those, only glucose (up to 75 g 1(-1)), ethanol (up to 4.9%, w/v) and glycerol (up to 6.6%, w/v) had positive effects on EPS production. EPS production was not directly linked to growth, because its production continued in the stationary phase provided that glucose was present. According to an empirical model, the growth of R parvulus 2.6 was completely inhibited by 58.9 +/- 18.1 gl(-1) lactate. Lactate, the sole fermentation product, was suggested to affect growth by chelation of manganese. The organism grew in an apparent linear fashion due to this imposed manganese limitation. This could be overcome by increasing the manganese concentration to at least 2 mg l(-1) in the medium. The excretion of Mn2+ upon depletion of glucose indicated that maintenance of the high Mn2+ gradient over the cell membrane is an energy requiring process. EPS production was increased from 0.12 gl(-1) to 4.10 gl(-1) in an improved medium that is based on the results from this study. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Velasco, Susana and Årsköld, Emma and Paese, Marco and Grage, Halfdan and Irastorza, A. and Rådström, Peter and van Niel, Ed},
  issn         = {0168-1605},
  keyword      = {growth,Pediococcus parvulus,exopolysaccharides,manganese},
  language     = {eng},
  number       = {3},
  pages        = {252--258},
  publisher    = {Elsevier},
  series       = {International Journal of Food Microbiology},
  title        = {Environmental factors influencing growth of and exopolysaccharide formation by Pediococcus parvulus 2.6},
  url          = {http://dx.doi.org/10.1016/j.ijfoodmicro.2006.06.008},
  volume       = {111},
  year         = {2006},
}