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Gene sco1417 encodes a positive regulator of the de novo biosynthesis of pyridoxal 5ʹ-phosphate (vitamin B6) in Streptomyces coelicolor M145

Tsypik, Olga ; Makitrynskyy, Roman ; Gauger, Elisabeth LU ; Mitulski, Agnieszka ; Bechthold, Andreas ; Flärdh, Klas LU ; Wohlleben, Wolfgang and Ostash, Bohdan (2025) In BMC Microbiology 25(1).
Abstract

Background: Actinomycetes of the genus Streptomyces are renowned for their highly developed and diverse specialized metaboliс pathways, and there is an extensive body of data on their specific and pleiotropic levels of regulation. Much less is known about routes leading to essential metabolites in this genus. In this work, we focused on elucidating the function of the highly conserved across Streptomyces gene SCO1417 for GntR type regulator in the model strain S. coelicolor A3(2). Results: Combining the results of knockout and promoter probe experiments, we show that the gene SCO1417 controls pyridoxal 5ʹ-phosphate (PLP; vitamin B6) biosynthesis, and thus is a member of the PdxR group of the transcriptional regulators. The Sco1417... (More)

Background: Actinomycetes of the genus Streptomyces are renowned for their highly developed and diverse specialized metaboliс pathways, and there is an extensive body of data on their specific and pleiotropic levels of regulation. Much less is known about routes leading to essential metabolites in this genus. In this work, we focused on elucidating the function of the highly conserved across Streptomyces gene SCO1417 for GntR type regulator in the model strain S. coelicolor A3(2). Results: Combining the results of knockout and promoter probe experiments, we show that the gene SCO1417 controls pyridoxal 5ʹ-phosphate (PLP; vitamin B6) biosynthesis, and thus is a member of the PdxR group of the transcriptional regulators. The Sco1417 protein is a transcriptional repressor of its gene and an activator of the expression of the PLP synthase genes, SCO1523 (pdxS) and SCO1522 (pdxT). According to electrophoretic mobility shift assays, out of several tested B6 vitamers, only PLP served as a Sco1417 effector molecule. We also provide data on the location of the Sco1417 binding site within the promoter region of pdxST. Conclusions: Our work portrays for the first time an evidence-based picture of the genetic control of vitamin B6 biosynthesis in S. coelicolor M145. Given the high conservancy and synteny of pdx homologs in the other streptomycetes, we suggest that the described genetic circuit is a general feature for the entire genus.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Essential metabolism, Genes, GntR type regulators, Mutation, PLP, Streptomyces coelicolor A3(2), Vitamin B6
in
BMC Microbiology
volume
25
issue
1
article number
383
publisher
BioMed Central (BMC)
external identifiers
  • pmid:40604380
  • scopus:105009957749
ISSN
1471-2180
DOI
10.1186/s12866-025-04108-y
language
English
LU publication?
yes
id
860f7e5f-4473-45bb-88bf-fb3113b9e01f
date added to LUP
2025-10-23 15:30:08
date last changed
2025-10-29 09:51:02
@article{860f7e5f-4473-45bb-88bf-fb3113b9e01f,
  abstract     = {{<p>Background: Actinomycetes of the genus Streptomyces are renowned for their highly developed and diverse specialized metaboliс pathways, and there is an extensive body of data on their specific and pleiotropic levels of regulation. Much less is known about routes leading to essential metabolites in this genus. In this work, we focused on elucidating the function of the highly conserved across Streptomyces gene SCO1417 for GntR type regulator in the model strain S. coelicolor A3(2). Results: Combining the results of knockout and promoter probe experiments, we show that the gene SCO1417 controls pyridoxal 5ʹ-phosphate (PLP; vitamin B6) biosynthesis, and thus is a member of the PdxR group of the transcriptional regulators. The Sco1417 protein is a transcriptional repressor of its gene and an activator of the expression of the PLP synthase genes, SCO1523 (pdxS) and SCO1522 (pdxT). According to electrophoretic mobility shift assays, out of several tested B6 vitamers, only PLP served as a Sco1417 effector molecule. We also provide data on the location of the Sco1417 binding site within the promoter region of pdxST. Conclusions: Our work portrays for the first time an evidence-based picture of the genetic control of vitamin B6 biosynthesis in S. coelicolor M145. Given the high conservancy and synteny of pdx homologs in the other streptomycetes, we suggest that the described genetic circuit is a general feature for the entire genus.</p>}},
  author       = {{Tsypik, Olga and Makitrynskyy, Roman and Gauger, Elisabeth and Mitulski, Agnieszka and Bechthold, Andreas and Flärdh, Klas and Wohlleben, Wolfgang and Ostash, Bohdan}},
  issn         = {{1471-2180}},
  keywords     = {{Essential metabolism; Genes; GntR type regulators; Mutation; PLP; Streptomyces coelicolor A3(2); Vitamin B6}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{BioMed Central (BMC)}},
  series       = {{BMC Microbiology}},
  title        = {{Gene sco1417 encodes a positive regulator of the de novo biosynthesis of pyridoxal 5ʹ-phosphate (vitamin B6) in Streptomyces coelicolor M145}},
  url          = {{http://dx.doi.org/10.1186/s12866-025-04108-y}},
  doi          = {{10.1186/s12866-025-04108-y}},
  volume       = {{25}},
  year         = {{2025}},
}