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The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells

Karlsson, Göran LU ; Rörby, Emma LU ; Pina, Cristina; Soneji, Shamit LU ; Reckzeh, Kristian; Miharada, Kenichi LU ; Karlsson, Christine LU ; Guo, Yanping; Fugazza, Cristina and Gupta, Rajeev, et al. (2013) In Cell Reports 4(4). p.642-648
Abstract
Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.
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Contribution to journal
publication status
published
subject
in
Cell Reports
volume
4
issue
4
pages
642 - 648
publisher
Cell Press
external identifiers
  • wos:000324285700004
  • scopus:84883264782
ISSN
2211-1247
DOI
10.1016/j.celrep.2013.07.020
language
English
LU publication?
yes
id
861cc339-9cf1-486d-993e-55b166ec633c (old id 4172665)
date added to LUP
2013-12-06 12:33:19
date last changed
2019-02-20 05:05:55
@article{861cc339-9cf1-486d-993e-55b166ec633c,
  abstract     = {Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.},
  author       = {Karlsson, Göran and Rörby, Emma and Pina, Cristina and Soneji, Shamit and Reckzeh, Kristian and Miharada, Kenichi and Karlsson, Christine and Guo, Yanping and Fugazza, Cristina and Gupta, Rajeev and Martens, Joost H. A. and Stunnenberg, Hendrik G. and Karlsson, Stefan and Enver, Tariq},
  issn         = {2211-1247},
  language     = {eng},
  number       = {4},
  pages        = {642--648},
  publisher    = {Cell Press},
  series       = {Cell Reports},
  title        = {The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells},
  url          = {http://dx.doi.org/10.1016/j.celrep.2013.07.020},
  volume       = {4},
  year         = {2013},
}