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Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.

Sjövall, Fredrik LU ; Ehinger, Johannes LU ; Marelsson, Sigurdur LU ; Morota, Saori LU ; Åsander Frostner, Eleonor LU ; Uchino, Hiroyuki ; Lundgren, Johan LU ; Arnbjörnsson, Einar LU ; Hansson, Magnus LU and Fellman, Vineta LU , et al. (2013) In Mitochondrion 13(1). p.7-14
Abstract
Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different... (More)
Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability. (Less)
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type
Contribution to journal
publication status
published
subject
in
Mitochondrion
volume
13
issue
1
pages
7 - 14
publisher
Elsevier
external identifiers
  • wos:000315757100002
  • pmid:23164798
  • scopus:84873304542
  • pmid:23164798
ISSN
1567-7249
DOI
10.1016/j.mito.2012.11.001
project
EU FP Horizon 2020 COST Action CA15203 MITOEAGLE
language
English
LU publication?
yes
id
8804a031-bacb-4f14-85ec-5041dd839bcb (old id 3218800)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23164798?dopt=Abstract
date added to LUP
2016-04-01 10:14:52
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2020-01-21 02:29:06
@article{8804a031-bacb-4f14-85ec-5041dd839bcb,
  abstract     = {Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.},
  author       = {Sjövall, Fredrik and Ehinger, Johannes and Marelsson, Sigurdur and Morota, Saori and Åsander Frostner, Eleonor and Uchino, Hiroyuki and Lundgren, Johan and Arnbjörnsson, Einar and Hansson, Magnus and Fellman, Vineta and Elmer, Eskil},
  issn         = {1567-7249},
  language     = {eng},
  number       = {1},
  pages        = {7--14},
  publisher    = {Elsevier},
  series       = {Mitochondrion},
  title        = {Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.},
  url          = {http://dx.doi.org/10.1016/j.mito.2012.11.001},
  doi          = {10.1016/j.mito.2012.11.001},
  volume       = {13},
  year         = {2013},
}