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Label-free concentration of viable neurons, hESCs and cancer cells by means of acoustophoresis.

Castro Zalis, Marina LU ; Reyes, Juan F LU ; Augustsson, Per LU ; Holmqvist, Staffan LU ; Roybon, Laurent LU ; Laurell, Thomas LU and Deierborg, Tomas LU (2016) In Integrative Biology 8(3). p.332-340
Abstract
Concentration of viable cell populations in suspension is of interest for several clinical and pre-clinical applications. Here, we report that microfluidic acoustophoresis is an effective method to efficiently concentrate live and viable cells with high target purity without any need for protein fluorescent labeling using antibodies or over-expression. We explored the effect of the acoustic field acoustic energy density and systematically used different protocols to induce apoptosis or cell death and then determined the efficiency of live and dead cell separation. We used the breast cancer cell line MCF-7, the mouse neuroblastoma N2a as well as human embryonic stem cells (hESCs) to demonstrate that this method is gentle and can be applied... (More)
Concentration of viable cell populations in suspension is of interest for several clinical and pre-clinical applications. Here, we report that microfluidic acoustophoresis is an effective method to efficiently concentrate live and viable cells with high target purity without any need for protein fluorescent labeling using antibodies or over-expression. We explored the effect of the acoustic field acoustic energy density and systematically used different protocols to induce apoptosis or cell death and then determined the efficiency of live and dead cell separation. We used the breast cancer cell line MCF-7, the mouse neuroblastoma N2a as well as human embryonic stem cells (hESCs) to demonstrate that this method is gentle and can be applied to different cell populations. First, we induced cell death by means of high osmotic shock using a high concentration of PBS (10×), the protein kinase inhibitor staurosporine, high concentrations of dimethyl sulfoxide (DMSO, 10%), and finally, cell starvation. In all the methods employed, we successfully induced cell death and were able to purify and concentrate the remaining live cells using acoustophoresis. Importantly, the concentration of viable cells was not dependent on a specific cell type. Further, we demonstrate that different death inducing stimuli have different effects on the intrinsic cell properties and therefore affect the efficiency of the acoustophoretic separation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Integrative Biology
volume
8
issue
3
pages
332 - 340
publisher
Royal Society of Chemistry
external identifiers
  • pmid:26915333
  • scopus:84960961317
  • wos:000372256400007
ISSN
1757-9708
DOI
10.1039/c5ib00288e
language
English
LU publication?
yes
id
ac318363-3704-402e-8c0c-f4cdfc8e3ef8 (old id 8821651)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26915333?dopt=Abstract
date added to LUP
2016-03-02 14:45:10
date last changed
2017-07-23 03:23:09
@article{ac318363-3704-402e-8c0c-f4cdfc8e3ef8,
  abstract     = {Concentration of viable cell populations in suspension is of interest for several clinical and pre-clinical applications. Here, we report that microfluidic acoustophoresis is an effective method to efficiently concentrate live and viable cells with high target purity without any need for protein fluorescent labeling using antibodies or over-expression. We explored the effect of the acoustic field acoustic energy density and systematically used different protocols to induce apoptosis or cell death and then determined the efficiency of live and dead cell separation. We used the breast cancer cell line MCF-7, the mouse neuroblastoma N2a as well as human embryonic stem cells (hESCs) to demonstrate that this method is gentle and can be applied to different cell populations. First, we induced cell death by means of high osmotic shock using a high concentration of PBS (10×), the protein kinase inhibitor staurosporine, high concentrations of dimethyl sulfoxide (DMSO, 10%), and finally, cell starvation. In all the methods employed, we successfully induced cell death and were able to purify and concentrate the remaining live cells using acoustophoresis. Importantly, the concentration of viable cells was not dependent on a specific cell type. Further, we demonstrate that different death inducing stimuli have different effects on the intrinsic cell properties and therefore affect the efficiency of the acoustophoretic separation.},
  author       = {Castro Zalis, Marina and Reyes, Juan F and Augustsson, Per and Holmqvist, Staffan and Roybon, Laurent and Laurell, Thomas and Deierborg, Tomas},
  issn         = {1757-9708},
  language     = {eng},
  month        = {02},
  number       = {3},
  pages        = {332--340},
  publisher    = {Royal Society of Chemistry},
  series       = {Integrative Biology},
  title        = {Label-free concentration of viable neurons, hESCs and cancer cells by means of acoustophoresis.},
  url          = {http://dx.doi.org/10.1039/c5ib00288e},
  volume       = {8},
  year         = {2016},
}