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Investigation of the metabolic consequences of impregnating spinach leaves with trehalose and applying a pulsed electric field.

Dymek, Katarzyna LU ; Panarese, Valentina ; Herremans, Els ; Cantre, Dennis ; Schoo, Rick ; Toraño, Javier Sastre ; Schluepmann, Henriette ; Wadsö, Lars LU ; Verboven, Pieter and Nicolai, Bart M , et al. (2016) In Bioelectrochemistry 112. p.153-157
Abstract
The impregnation of leafy vegetables with cryoprotectants using a combination of vacuum impregnation (VI) and pulsed electric fields (PEF) has been proposed by our research group as a method of improving their freezing tolerance and consequently their general quality after thawing. In this study, we have investigated the metabolic consequences of the combination of these unit operations on spinach. The vacuum impregnated spinach leaves showed a drastic decrease in the porosity of the extracellular space. However, at maximum weight gain, randomly located air pockets remained, which may account for oxygen-consuming pathways in the cells being active after VI. The metabolic activity of the impregnated leaves showed a drastic increase that was... (More)
The impregnation of leafy vegetables with cryoprotectants using a combination of vacuum impregnation (VI) and pulsed electric fields (PEF) has been proposed by our research group as a method of improving their freezing tolerance and consequently their general quality after thawing. In this study, we have investigated the metabolic consequences of the combination of these unit operations on spinach. The vacuum impregnated spinach leaves showed a drastic decrease in the porosity of the extracellular space. However, at maximum weight gain, randomly located air pockets remained, which may account for oxygen-consuming pathways in the cells being active after VI. The metabolic activity of the impregnated leaves showed a drastic increase that was further enhanced by the application of PEF to the impregnated tissue. Impregnating the leaves with trehalose by VI led to a significant accumulation of trehalose-6-phosphate (T6P), however, this was not further enhanced by PEF. It is suggested that the accumulation of T6P in the leaves may increase metabolic activity, and increase tissue resistance to abiotic stress. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioelectrochemistry
volume
112
pages
5 pages
publisher
Elsevier
external identifiers
  • pmid:26936113
  • pmid:26936113
  • scopus:84975736508
  • wos:000383523800020
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2016.02.006
language
English
LU publication?
yes
id
f888f614-80d1-4a1c-b1ce-6f4dc5e9ed66 (old id 8856435)
date added to LUP
2016-04-01 10:39:06
date last changed
2025-04-04 14:58:12
@article{f888f614-80d1-4a1c-b1ce-6f4dc5e9ed66,
  abstract     = {{The impregnation of leafy vegetables with cryoprotectants using a combination of vacuum impregnation (VI) and pulsed electric fields (PEF) has been proposed by our research group as a method of improving their freezing tolerance and consequently their general quality after thawing. In this study, we have investigated the metabolic consequences of the combination of these unit operations on spinach. The vacuum impregnated spinach leaves showed a drastic decrease in the porosity of the extracellular space. However, at maximum weight gain, randomly located air pockets remained, which may account for oxygen-consuming pathways in the cells being active after VI. The metabolic activity of the impregnated leaves showed a drastic increase that was further enhanced by the application of PEF to the impregnated tissue. Impregnating the leaves with trehalose by VI led to a significant accumulation of trehalose-6-phosphate (T6P), however, this was not further enhanced by PEF. It is suggested that the accumulation of T6P in the leaves may increase metabolic activity, and increase tissue resistance to abiotic stress.}},
  author       = {{Dymek, Katarzyna and Panarese, Valentina and Herremans, Els and Cantre, Dennis and Schoo, Rick and Toraño, Javier Sastre and Schluepmann, Henriette and Wadsö, Lars and Verboven, Pieter and Nicolai, Bart M and Dejmek, Petr and Gomez, Federico}},
  issn         = {{1878-562X}},
  language     = {{eng}},
  pages        = {{153--157}},
  publisher    = {{Elsevier}},
  series       = {{Bioelectrochemistry}},
  title        = {{Investigation of the metabolic consequences of impregnating spinach leaves with trehalose and applying a pulsed electric field.}},
  url          = {{http://dx.doi.org/10.1016/j.bioelechem.2016.02.006}},
  doi          = {{10.1016/j.bioelechem.2016.02.006}},
  volume       = {{112}},
  year         = {{2016}},
}