MicroRNAs in Type 2 Diabetes : Focus on MicroRNA Profiling in Islets of Langerhans
(2022) In Methods in molecular biology (Clifton, N.J.) p.113-142- Abstract
Differential expression of microRNAs (miRNAs) is observed in many diseases including type 2 diabetes (T2D). Insulin secretion from pancreatic beta cells is central for the regulation of blood glucose levels and failure to release enough insulin results in hyperglycemia and T2D. The importance in T2D pathogenesis of single miRNAs in beta cells has been described; however, to get the full picture, high-throughput miRNA sequencing is necessary. Here we describe a method using small RNA sequencing, from sample preparation to expression analysis using bioinformatic tools. In the end, a tutorial on differential expression analysis is presented in R using publicly available data.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/96d9df58-b075-4368-a0e5-4d372da98973
- author
- Cowan, Elaine LU ; Karagiannopoulos, Alexandros LU and Eliasson, Lena LU
- organization
- publishing date
- 2022-12-13
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Islets of Langerhans, Beta-cell, Insulin secretion, MicroRNA, Diabetes, Cardiometabolic disease, RNA sequencing, Short noncoding RNA, Expression analyses
- host publication
- Type-1 Diabetes : Methods and Protocols - Methods and Protocols
- series title
- Methods in molecular biology (Clifton, N.J.)
- editor
- Moore, Anna and Wang, Ping
- issue
- 2592
- pages
- 30 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:36507989
- scopus:85144388229
- ISSN
- 1940-6029
- ISBN
- 978-1-0716-2807-2
- 978-1-0716-2806-5
- DOI
- 10.1007/978-1-0716-2807-2_8
- language
- English
- LU publication?
- yes
- additional info
- © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- id
- 96d9df58-b075-4368-a0e5-4d372da98973
- date added to LUP
- 2022-12-14 10:44:30
- date last changed
- 2024-09-19 19:15:29
@inbook{96d9df58-b075-4368-a0e5-4d372da98973, abstract = {{<p>Differential expression of microRNAs (miRNAs) is observed in many diseases including type 2 diabetes (T2D). Insulin secretion from pancreatic beta cells is central for the regulation of blood glucose levels and failure to release enough insulin results in hyperglycemia and T2D. The importance in T2D pathogenesis of single miRNAs in beta cells has been described; however, to get the full picture, high-throughput miRNA sequencing is necessary. Here we describe a method using small RNA sequencing, from sample preparation to expression analysis using bioinformatic tools. In the end, a tutorial on differential expression analysis is presented in R using publicly available data.</p>}}, author = {{Cowan, Elaine and Karagiannopoulos, Alexandros and Eliasson, Lena}}, booktitle = {{Type-1 Diabetes : Methods and Protocols}}, editor = {{Moore, Anna and Wang, Ping}}, isbn = {{978-1-0716-2807-2}}, issn = {{1940-6029}}, keywords = {{Islets of Langerhans; Beta-cell; Insulin secretion; MicroRNA; Diabetes; Cardiometabolic disease; RNA sequencing; Short noncoding RNA; Expression analyses}}, language = {{eng}}, month = {{12}}, number = {{2592}}, pages = {{113--142}}, publisher = {{Humana Press}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{MicroRNAs in Type 2 Diabetes : Focus on MicroRNA Profiling in Islets of Langerhans}}, url = {{http://dx.doi.org/10.1007/978-1-0716-2807-2_8}}, doi = {{10.1007/978-1-0716-2807-2_8}}, year = {{2022}}, }