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Rat intrastriatal neural allografts challenged with skin allografts at different time points

Duan, W M LU ; Cameron, Reynolds M; Brundin, P LU and Widner, H LU (1997) In Experimental Neurology 148(1). p.47-334
Abstract

The present study was designed to address two questions. First, can an intrastriatal neural allograft exhibit long-term survival (18 weeks) if the host is immunized by an orthotopic skin graft 6 weeks after neural transplantation (the 6w-Long group)? Second, can an intrastriatal neural allograft survive when the host is challenged by an orthotopic skin allograft either simultaneously (Sim) with the intracerebral graft surgery or 2 (2w) weeks later? Dissociated embryonic ventral mesencephalic tissue from Lewis rats was stereotaxically injected into the striatum of Sprague-Dawley rats with unilateral 6-hydroxydopamine lesions. Six weeks after neural grafting, no reduction in amphetamine-induced motor asymmetry was observed in the Sim and... (More)

The present study was designed to address two questions. First, can an intrastriatal neural allograft exhibit long-term survival (18 weeks) if the host is immunized by an orthotopic skin graft 6 weeks after neural transplantation (the 6w-Long group)? Second, can an intrastriatal neural allograft survive when the host is challenged by an orthotopic skin allograft either simultaneously (Sim) with the intracerebral graft surgery or 2 (2w) weeks later? Dissociated embryonic ventral mesencephalic tissue from Lewis rats was stereotaxically injected into the striatum of Sprague-Dawley rats with unilateral 6-hydroxydopamine lesions. Six weeks after neural grafting, no reduction in amphetamine-induced motor asymmetry was observed in the Sim and 2w groups. At 6 weeks after skin grafting, the mean motor asymmetry scores had returned to the initial pretransplantation levels in the 6w-Long group. All the neural allografts in the Sim group were completely rejected, and the mean number of tyrosine hydroxylase immunoreactivity neurons in the grafts was significantly reduced in the 2w and the 6w-Long group, when compared to the no-skin control group. There were very high levels of expression of MHC class I and II antigens, marked cellular infiltrates containing macrophages and T-lymphocytes, and several activated microglia and astrocytes in and around the surviving intracerebral transplants in the 2w and the 6w-Long groups. The results suggest that intrastriatal neural allografts are more likely to be rejected rapidly if the host is efficiently immunized with the same alloantigens simultaneously or soon after the neural transplantation than at a later time point. When established neural allografts are subjected to a strong immunological challenge, they undergo protracted rejection.

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organization
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Contribution to journal
publication status
published
subject
keywords
Amphetamine, Animals, Astrocytes, Brain Tissue Transplantation, Cell Count, Corpus Striatum, Female, Fetal Tissue Transplantation, Glial Fibrillary Acidic Protein, Graft Rejection, Histocompatibility Antigens, Immunization, Locomotion, Mesencephalon, Nerve Tissue Proteins, Neurons, Oxidopamine, Postoperative Period, Rats, Rats, Inbred Lew, Rats, Sprague-Dawley, Skin Transplantation, Transplantation, Homologous, Tyrosine 3-Monooxygenase, Journal Article, Research Support, Non-U.S. Gov't
in
Experimental Neurology
volume
148
issue
1
pages
14 pages
publisher
Academic Press
external identifiers
  • scopus:0031264159
ISSN
0014-4886
DOI
10.1006/exnr.1997.6656
language
English
LU publication?
yes
id
99f19016-22f5-4fbe-a0a5-c158c58f98bb
date added to LUP
2017-04-19 18:25:45
date last changed
2017-04-28 12:22:10
@article{99f19016-22f5-4fbe-a0a5-c158c58f98bb,
  abstract     = {<p>The present study was designed to address two questions. First, can an intrastriatal neural allograft exhibit long-term survival (18 weeks) if the host is immunized by an orthotopic skin graft 6 weeks after neural transplantation (the 6w-Long group)? Second, can an intrastriatal neural allograft survive when the host is challenged by an orthotopic skin allograft either simultaneously (Sim) with the intracerebral graft surgery or 2 (2w) weeks later? Dissociated embryonic ventral mesencephalic tissue from Lewis rats was stereotaxically injected into the striatum of Sprague-Dawley rats with unilateral 6-hydroxydopamine lesions. Six weeks after neural grafting, no reduction in amphetamine-induced motor asymmetry was observed in the Sim and 2w groups. At 6 weeks after skin grafting, the mean motor asymmetry scores had returned to the initial pretransplantation levels in the 6w-Long group. All the neural allografts in the Sim group were completely rejected, and the mean number of tyrosine hydroxylase immunoreactivity neurons in the grafts was significantly reduced in the 2w and the 6w-Long group, when compared to the no-skin control group. There were very high levels of expression of MHC class I and II antigens, marked cellular infiltrates containing macrophages and T-lymphocytes, and several activated microglia and astrocytes in and around the surviving intracerebral transplants in the 2w and the 6w-Long groups. The results suggest that intrastriatal neural allografts are more likely to be rejected rapidly if the host is efficiently immunized with the same alloantigens simultaneously or soon after the neural transplantation than at a later time point. When established neural allografts are subjected to a strong immunological challenge, they undergo protracted rejection.</p>},
  author       = {Duan, W M and Cameron, Reynolds M and Brundin, P and Widner, H},
  issn         = {0014-4886},
  keyword      = {Amphetamine,Animals,Astrocytes,Brain Tissue Transplantation,Cell Count,Corpus Striatum,Female,Fetal Tissue Transplantation,Glial Fibrillary Acidic Protein,Graft Rejection,Histocompatibility Antigens,Immunization,Locomotion,Mesencephalon,Nerve Tissue Proteins,Neurons,Oxidopamine,Postoperative Period,Rats,Rats, Inbred Lew,Rats, Sprague-Dawley,Skin Transplantation,Transplantation, Homologous,Tyrosine 3-Monooxygenase,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {47--334},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Rat intrastriatal neural allografts challenged with skin allografts at different time points},
  url          = {http://dx.doi.org/10.1006/exnr.1997.6656},
  volume       = {148},
  year         = {1997},
}