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A comparison between pulsed electric field and moderate electric field for their effectiveness in improving the freezing tolerance of rocket leaves

Nyoto, Imelda Christiana LU and Gómez Galindo, Federico LU (2023) In Biochemistry and Biophysics Reports 35.
Abstract

Two electrotechnologies: pulsed electric fields (PEF) and moderate electric field (MEF) in combination with vacuum impregnation of glycerol as cryoprotectant were used to increase the freezing tolerance of rocket leaves. Rocket leaves were treated with PEF using a nominal field strength of 1200 V/cm or MEF at different combinations of voltage and frequency. Leaves were then immersed in a glycerol solution at 32, 36 and 40% (w/v) under vacuum for 26 min. After this treatment, the leaves were allowed to rest for 3 days before they were frozen and thawed. Leaf survival was assessed at different time points after thawing with microscopic observations and wilting tests. When the viability of the leaves was assessed 5 min after thawing,... (More)

Two electrotechnologies: pulsed electric fields (PEF) and moderate electric field (MEF) in combination with vacuum impregnation of glycerol as cryoprotectant were used to increase the freezing tolerance of rocket leaves. Rocket leaves were treated with PEF using a nominal field strength of 1200 V/cm or MEF at different combinations of voltage and frequency. Leaves were then immersed in a glycerol solution at 32, 36 and 40% (w/v) under vacuum for 26 min. After this treatment, the leaves were allowed to rest for 3 days before they were frozen and thawed. Leaf survival was assessed at different time points after thawing with microscopic observations and wilting tests. When the viability of the leaves was assessed 5 min after thawing, 60–68% of the leaves in the batch survived. There was no difference in the levels of surviving leaves when PEF and the lowest-tested voltage used in MEF were tested. However, from the leaves surviving 5 min after thawing, approximately half of them die over a 24 h period after thawing.

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author
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organization
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type
Contribution to journal
publication status
published
subject
keywords
Cross-tolerance, Electroporation, Freeze-thaw survival, Stress
in
Biochemistry and Biophysics Reports
volume
35
article number
101515
publisher
Elsevier
external identifiers
  • pmid:37601450
  • scopus:85165093310
ISSN
2405-5808
DOI
10.1016/j.bbrep.2023.101515
language
English
LU publication?
yes
id
9a080d7c-3ea6-4bc7-a852-47431d0961c6
date added to LUP
2023-09-05 13:41:44
date last changed
2024-04-20 02:39:56
@article{9a080d7c-3ea6-4bc7-a852-47431d0961c6,
  abstract     = {{<p>Two electrotechnologies: pulsed electric fields (PEF) and moderate electric field (MEF) in combination with vacuum impregnation of glycerol as cryoprotectant were used to increase the freezing tolerance of rocket leaves. Rocket leaves were treated with PEF using a nominal field strength of 1200 V/cm or MEF at different combinations of voltage and frequency. Leaves were then immersed in a glycerol solution at 32, 36 and 40% (w/v) under vacuum for 26 min. After this treatment, the leaves were allowed to rest for 3 days before they were frozen and thawed. Leaf survival was assessed at different time points after thawing with microscopic observations and wilting tests. When the viability of the leaves was assessed 5 min after thawing, 60–68% of the leaves in the batch survived. There was no difference in the levels of surviving leaves when PEF and the lowest-tested voltage used in MEF were tested. However, from the leaves surviving 5 min after thawing, approximately half of them die over a 24 h period after thawing.</p>}},
  author       = {{Nyoto, Imelda Christiana and Gómez Galindo, Federico}},
  issn         = {{2405-5808}},
  keywords     = {{Cross-tolerance; Electroporation; Freeze-thaw survival; Stress}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Biochemistry and Biophysics Reports}},
  title        = {{A comparison between pulsed electric field and moderate electric field for their effectiveness in improving the freezing tolerance of rocket leaves}},
  url          = {{http://dx.doi.org/10.1016/j.bbrep.2023.101515}},
  doi          = {{10.1016/j.bbrep.2023.101515}},
  volume       = {{35}},
  year         = {{2023}},
}