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Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline

Sala, Simone LU ; Zhang, Yuhe LU ; De La Rosa, Nathaly LU ; Dreier, Till LU orcid ; Kahnt, Maik LU orcid ; Langer, Max ; Dahlin, Lars LU orcid ; Bech, Martin LU orcid ; Villanueva Perez, Pablo LU orcid and Kalbfleisch, Sebastian LU (2022) In Journal of Synchrotron Radiation 29(3).
Abstract
X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide... (More)
X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view. (Less)
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; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Synchrotron Radiation
volume
29
issue
3
publisher
International Union of Crystallography
external identifiers
  • scopus:85129998189
  • pmid:35511013
ISSN
1600-5775
DOI
10.1107/S1600577522001874
language
English
LU publication?
yes
id
9cce0e72-d19c-450e-ac9d-7f2ef2fb39d3
date added to LUP
2022-04-27 21:16:05
date last changed
2023-11-19 01:39:26
@article{9cce0e72-d19c-450e-ac9d-7f2ef2fb39d3,
  abstract     = {{X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.}},
  author       = {{Sala, Simone and Zhang, Yuhe and De La Rosa, Nathaly and Dreier, Till and Kahnt, Maik and Langer, Max and Dahlin, Lars and Bech, Martin and Villanueva Perez, Pablo and Kalbfleisch, Sebastian}},
  issn         = {{1600-5775}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{3}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Synchrotron Radiation}},
  title        = {{Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline}},
  url          = {{http://dx.doi.org/10.1107/S1600577522001874}},
  doi          = {{10.1107/S1600577522001874}},
  volume       = {{29}},
  year         = {{2022}},
}