Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
(2022) In Journal of Synchrotron Radiation 29(3).- Abstract
- X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide... (More)
- X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/9cce0e72-d19c-450e-ac9d-7f2ef2fb39d3
- author
- Sala, Simone LU ; Zhang, Yuhe LU ; De La Rosa, Nathaly LU ; Dreier, Till LU ; Kahnt, Maik LU ; Langer, Max ; Dahlin, Lars LU ; Bech, Martin LU ; Villanueva Perez, Pablo LU and Kalbfleisch, Sebastian LU
- organization
-
- MAX IV Laboratory
- Synchrotron Radiation Research
- NanoLund: Centre for Nanoscience
- Medical Radiation Physics, Lund
- EpiHealth: Epidemiology for Health
- WCMM-Wallenberg Centre for Molecular Medicine
- Department of Translational Medicine
- Hand Surgery, Malmö (research group)
- X-ray Phase Contrast (research group)
- publishing date
- 2022-03-16
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Synchrotron Radiation
- volume
- 29
- issue
- 3
- publisher
- International Union of Crystallography
- external identifiers
-
- scopus:85129998189
- pmid:35511013
- ISSN
- 1600-5775
- DOI
- 10.1107/S1600577522001874
- language
- English
- LU publication?
- yes
- id
- 9cce0e72-d19c-450e-ac9d-7f2ef2fb39d3
- date added to LUP
- 2022-04-27 21:16:05
- date last changed
- 2023-11-19 01:39:26
@article{9cce0e72-d19c-450e-ac9d-7f2ef2fb39d3, abstract = {{X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.}}, author = {{Sala, Simone and Zhang, Yuhe and De La Rosa, Nathaly and Dreier, Till and Kahnt, Maik and Langer, Max and Dahlin, Lars and Bech, Martin and Villanueva Perez, Pablo and Kalbfleisch, Sebastian}}, issn = {{1600-5775}}, language = {{eng}}, month = {{03}}, number = {{3}}, publisher = {{International Union of Crystallography}}, series = {{Journal of Synchrotron Radiation}}, title = {{Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline}}, url = {{http://dx.doi.org/10.1107/S1600577522001874}}, doi = {{10.1107/S1600577522001874}}, volume = {{29}}, year = {{2022}}, }