Probing Cage Relaxation in Concentrated Protein Solutions by X-Ray Photon Correlation Spectroscopy
Chushkin, Yuriy ; Gulotta, Alessandro LU ; Roosen-Runge, Felix LU ; Pal, Antara LU ; Stradner, Anna LU and Schurtenberger, Peter LU
(2022)
In Physical Review Letters
129(23).
- Abstract
Diffusion of proteins on length scales of their size is crucial for understanding the machinery of living cells. X-ray photon correlation spectroscopy (XPCS) is currently the only way to access long-time collective diffusion on these length scales, but radiation damage so far limits the use in biological systems. We apply a new approach to use XPCS to measure cage relaxation in crowded α-crystallin solutions. This allows us to correct for radiation effects, obtain missing information on long time diffusion, and support the fundamental analogy between protein and colloid dynamical arrest.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/9f81b747-7e89-4068-90ad-1f7ce3094495
- author
- Chushkin, Yuriy
; Gulotta, Alessandro
LU
; Roosen-Runge, Felix
LU
; Pal, Antara
LU
; Stradner, Anna
LU
and Schurtenberger, Peter
LU
- organization
- publishing date
- 2022-12-02
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physical Review Letters
- volume
- 129
- issue
- 23
- article number
- 238001
- pages
- 6 pages
- publisher
- American Physical Society
- external identifiers
-
- pmid:36563210
- scopus:85143738867
- ISSN
- 0031-9007
- DOI
- 10.1103/PhysRevLett.129.238001
- language
- English
- LU publication?
- yes
- id
- 9f81b747-7e89-4068-90ad-1f7ce3094495
- date added to LUP
- 2023-08-14 10:23:22
- date last changed
- 2024-04-20 00:30:47
@article{9f81b747-7e89-4068-90ad-1f7ce3094495,
abstract = {{<p>Diffusion of proteins on length scales of their size is crucial for understanding the machinery of living cells. X-ray photon correlation spectroscopy (XPCS) is currently the only way to access long-time collective diffusion on these length scales, but radiation damage so far limits the use in biological systems. We apply a new approach to use XPCS to measure cage relaxation in crowded α-crystallin solutions. This allows us to correct for radiation effects, obtain missing information on long time diffusion, and support the fundamental analogy between protein and colloid dynamical arrest.</p>}},
author = {{Chushkin, Yuriy and Gulotta, Alessandro and Roosen-Runge, Felix and Pal, Antara and Stradner, Anna and Schurtenberger, Peter}},
issn = {{0031-9007}},
language = {{eng}},
month = {{12}},
number = {{23}},
publisher = {{American Physical Society}},
series = {{Physical Review Letters}},
title = {{Probing Cage Relaxation in Concentrated Protein Solutions by X-Ray Photon Correlation Spectroscopy}},
url = {{http://dx.doi.org/10.1103/PhysRevLett.129.238001}},
doi = {{10.1103/PhysRevLett.129.238001}},
volume = {{129}},
year = {{2022}},
}