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Effects of verapamil on amylase release from rat pancreatic acini and its relation to calcium fluxes.

Duan, Rui-Dong LU and Erlanson-Albertsson, Charlotte LU (1988) In Pancreas 3(4). p.427-432
Abstract
The effects of verapamil on amylase release and Ca2+ fluxes from rat pancreatic acini have been studied. Verapamil at concentrations above 10 FM dose-dependently inhibited amylase release stimulated by carbachol, but enhanced the amylase release stimulated by cholecystokinin (CCK) and secretin. Verapamil had no significant effect on calcium uptake induced by carbachol or CCK, but significantly inhibited Caf2 eflux caused by carbachol and slightly increased that caused by CCK. In a Ca2+-free, EDTA-containing medium, the increase in cytoplasmic free Ca2+ caused by carbachol was significantly inhibited by verapamil. Verapamil alone up to 400 p, M had no effect on the release of lactic dehydrogenase. In conclusion, the effect of verapamil on... (More)
The effects of verapamil on amylase release and Ca2+ fluxes from rat pancreatic acini have been studied. Verapamil at concentrations above 10 FM dose-dependently inhibited amylase release stimulated by carbachol, but enhanced the amylase release stimulated by cholecystokinin (CCK) and secretin. Verapamil had no significant effect on calcium uptake induced by carbachol or CCK, but significantly inhibited Caf2 eflux caused by carbachol and slightly increased that caused by CCK. In a Ca2+-free, EDTA-containing medium, the increase in cytoplasmic free Ca2+ caused by carbachol was significantly inhibited by verapamil. Verapamil alone up to 400 p, M had no effect on the release of lactic dehydrogenase. In conclusion, the effect of verapamil on amylase release from rat pancreatic acini differs depending on the type of secretagogue used to stimulate amylase release. This effect is not related to blockage of Ca2+ uptake, indicating another mechanism of verapamil on pancreatic acini. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pancreas
volume
3
issue
4
pages
5 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0024269017
ISSN
0885-3177
DOI
10.1097/00006676-198808000-00010
language
English
LU publication?
yes
id
9fed4d93-67c3-4b6b-ac6c-67511b770ac0
date added to LUP
2019-02-02 16:43:14
date last changed
2025-04-04 15:17:51
@article{9fed4d93-67c3-4b6b-ac6c-67511b770ac0,
  abstract     = {{The effects of verapamil on amylase release and Ca2+ fluxes from rat pancreatic acini have been studied. Verapamil at concentrations above 10 FM dose-dependently inhibited amylase release stimulated by carbachol, but enhanced the amylase release stimulated by cholecystokinin (CCK) and secretin. Verapamil had no significant effect on calcium uptake induced by carbachol or CCK, but significantly inhibited Caf2 eflux caused by carbachol and slightly increased that caused by CCK. In a Ca2+-free, EDTA-containing medium, the increase in cytoplasmic free Ca2+ caused by carbachol was significantly inhibited by verapamil. Verapamil alone up to 400 p, M had no effect on the release of lactic dehydrogenase. In conclusion, the effect of verapamil on amylase release from rat pancreatic acini differs depending on the type of secretagogue used to stimulate amylase release. This effect is not related to blockage of Ca2+ uptake, indicating another mechanism of verapamil on pancreatic acini.}},
  author       = {{Duan, Rui-Dong and Erlanson-Albertsson, Charlotte}},
  issn         = {{0885-3177}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{427--432}},
  publisher    = {{Lippincott Williams & Wilkins}},
  series       = {{Pancreas}},
  title        = {{Effects of verapamil on amylase release from rat pancreatic acini and its relation to calcium fluxes.}},
  url          = {{http://dx.doi.org/10.1097/00006676-198808000-00010}},
  doi          = {{10.1097/00006676-198808000-00010}},
  volume       = {{3}},
  year         = {{1988}},
}