Modeling of spectral changes for depth localization of fluorescent inclusion
(2005) In Optics Express 13(11). p.4263-4274- Abstract
- We have performed modeling of fluorescence signals from inclusions inside turbid media to investigate the influence of a limited fluorescence contrast and how accurately the depth can be determined by using the spectral information. The depth was determined by forming a ratio of simulated fluorescence intensities at two wavelengths. The results show that it is important to consider the background autofluorescence in determining the depth of a fluorescent inclusion. It is also necessary to know the optical properties of the tissue to obtain the depth. A 20% error in absorption or scattering coefficients yields an error in the determined depth of approximately 2-3 mm (relative error of 10-15%) in a 20 mm thick tissue slab.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/238674
- author
- Svensson, Jenny LU and Andersson-Engels, Stefan LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Optics Express
- volume
- 13
- issue
- 11
- pages
- 4263 - 4274
- publisher
- Optical Society of America
- external identifiers
-
- wos:000229534500043
- scopus:21244469457
- ISSN
- 1094-4087
- language
- English
- LU publication?
- yes
- id
- a0214f6d-85d6-4743-992e-a87481a38304 (old id 238674)
- alternative location
- http://www.opticsexpress.org/abstract.cfm?id=84168
- date added to LUP
- 2016-04-01 16:43:14
- date last changed
- 2022-01-28 21:39:30
@article{a0214f6d-85d6-4743-992e-a87481a38304, abstract = {{We have performed modeling of fluorescence signals from inclusions inside turbid media to investigate the influence of a limited fluorescence contrast and how accurately the depth can be determined by using the spectral information. The depth was determined by forming a ratio of simulated fluorescence intensities at two wavelengths. The results show that it is important to consider the background autofluorescence in determining the depth of a fluorescent inclusion. It is also necessary to know the optical properties of the tissue to obtain the depth. A 20% error in absorption or scattering coefficients yields an error in the determined depth of approximately 2-3 mm (relative error of 10-15%) in a 20 mm thick tissue slab.}}, author = {{Svensson, Jenny and Andersson-Engels, Stefan}}, issn = {{1094-4087}}, language = {{eng}}, number = {{11}}, pages = {{4263--4274}}, publisher = {{Optical Society of America}}, series = {{Optics Express}}, title = {{Modeling of spectral changes for depth localization of fluorescent inclusion}}, url = {{https://lup.lub.lu.se/search/files/4760288/2370794.pdf}}, volume = {{13}}, year = {{2005}}, }