Gastrointestinal conditions influence the solution behaviour of cereal beta-glucans in vitro
(2012) In Food Chemistry 130(3). p.536-540- Abstract
- The solution behaviour of b-glucans in a gastrointestinal model was investigated in order to explore the mechanisms explaining the physiological effects of the soluble fibre. Asymmetrical flow field-flow fractionation was used to determine the molar mass distribution, and in-line calcofluor labelling allowed specific
detection of b-glucans in complex samples. When dispersed in water, weight-average molar mass (Mw) was determined to 1 * 10^6 g/mol for pure oat and barley b-glucans, and 200 * 10^6 g/mol for b-glucans
in oat bran, indicating that the b-glucans were aggregating. Samples from the gastric digestion displayed disrupted aggregates, while samples from the small intestinal digestion contained re-formed
... (More) - The solution behaviour of b-glucans in a gastrointestinal model was investigated in order to explore the mechanisms explaining the physiological effects of the soluble fibre. Asymmetrical flow field-flow fractionation was used to determine the molar mass distribution, and in-line calcofluor labelling allowed specific
detection of b-glucans in complex samples. When dispersed in water, weight-average molar mass (Mw) was determined to 1 * 10^6 g/mol for pure oat and barley b-glucans, and 200 * 10^6 g/mol for b-glucans
in oat bran, indicating that the b-glucans were aggregating. Samples from the gastric digestion displayed disrupted aggregates, while samples from the small intestinal digestion contained re-formed
aggregates. Additionally, the aggregates from pure b-glucans were considerably denser after intestinal digestion. This may be construed as gel-formation in the small intestine, which should be tested for its relevance to health effects. Our results signal the difficulties in predicting b-glucan activity in the gastrointestinal tract purely from analysis of the fibre-rich product. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2277726
- author
- Ulmius, Matilda LU ; Adapa, Srimannarayana ; Önning, Gunilla LU and Nilsson, Lars LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cereal b-glucan, Oat bran, Field-flow fractionation, Gastrointestinal model, Calcofluor
- in
- Food Chemistry
- volume
- 130
- issue
- 3
- pages
- 536 - 540
- publisher
- Elsevier
- external identifiers
-
- wos:000296039400009
- scopus:80052698795
- ISSN
- 1873-7072
- DOI
- 10.1016/j.foodchem.2011.07.066
- language
- English
- LU publication?
- yes
- id
- a8a94d8a-8da7-4a86-8f6a-22c4a7acc432 (old id 2277726)
- date added to LUP
- 2016-04-01 10:15:01
- date last changed
- 2023-11-09 16:00:55
@article{a8a94d8a-8da7-4a86-8f6a-22c4a7acc432,
abstract = {{The solution behaviour of b-glucans in a gastrointestinal model was investigated in order to explore the mechanisms explaining the physiological effects of the soluble fibre. Asymmetrical flow field-flow fractionation was used to determine the molar mass distribution, and in-line calcofluor labelling allowed specific<br/><br>
detection of b-glucans in complex samples. When dispersed in water, weight-average molar mass (Mw) was determined to 1 * 10^6 g/mol for pure oat and barley b-glucans, and 200 * 10^6 g/mol for b-glucans<br/><br>
in oat bran, indicating that the b-glucans were aggregating. Samples from the gastric digestion displayed disrupted aggregates, while samples from the small intestinal digestion contained re-formed<br/><br>
aggregates. Additionally, the aggregates from pure b-glucans were considerably denser after intestinal digestion. This may be construed as gel-formation in the small intestine, which should be tested for its relevance to health effects. Our results signal the difficulties in predicting b-glucan activity in the gastrointestinal tract purely from analysis of the fibre-rich product.}},
author = {{Ulmius, Matilda and Adapa, Srimannarayana and Önning, Gunilla and Nilsson, Lars}},
issn = {{1873-7072}},
keywords = {{Cereal b-glucan; Oat bran; Field-flow fractionation; Gastrointestinal model; Calcofluor}},
language = {{eng}},
number = {{3}},
pages = {{536--540}},
publisher = {{Elsevier}},
series = {{Food Chemistry}},
title = {{Gastrointestinal conditions influence the solution behaviour of cereal beta-glucans in vitro}},
url = {{http://dx.doi.org/10.1016/j.foodchem.2011.07.066}},
doi = {{10.1016/j.foodchem.2011.07.066}},
volume = {{130}},
year = {{2012}},
}