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Relationship between HMF intake and SMF formation in vivo : An animal and human study

Pastoriza de la Cueva, Silvia; Álvarez, Juana; Végvári, Ákos LU ; Montilla-Gómez, Javier; Cruz-López, Olga; Delgado-Andrade, Cristina and Rufián-Henares, José A. (2016) In Molecular Nutrition and Food Research
Abstract

Scope: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. Methods and results: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology... (More)

Scope: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. Methods and results: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes. Conclusion: This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.

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Contribution to journal
publication status
epub
subject
keywords
5-Hydroxymethylfurfural, 5-Sulfooxymethylfurfural, Estimated intake, Heat processing, Maillard reaction
in
Molecular Nutrition and Food Research
publisher
John Wiley & Sons
external identifiers
  • scopus:85009230929
  • wos:000398095000010
ISSN
1613-4125
DOI
10.1002/mnfr.201600773
language
English
LU publication?
yes
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a9eeae59-77cd-4111-bc4f-752865257d90
date added to LUP
2017-02-21 12:02:51
date last changed
2017-09-18 11:34:00
@article{a9eeae59-77cd-4111-bc4f-752865257d90,
  abstract     = {<p>Scope: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. Methods and results: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes. Conclusion: This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.</p>},
  author       = {Pastoriza de la Cueva, Silvia and Álvarez, Juana and Végvári, Ákos and Montilla-Gómez, Javier and Cruz-López, Olga and Delgado-Andrade, Cristina and Rufián-Henares, José A.},
  issn         = {1613-4125},
  keyword      = {5-Hydroxymethylfurfural,5-Sulfooxymethylfurfural,Estimated intake,Heat processing,Maillard reaction},
  language     = {eng},
  month        = {12},
  publisher    = {John Wiley & Sons},
  series       = {Molecular Nutrition and Food Research},
  title        = {Relationship between HMF intake and SMF formation in vivo : An animal and human study},
  url          = {http://dx.doi.org/10.1002/mnfr.201600773},
  year         = {2016},
}