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Methylprednisolone prevents rejection of intrastriatal grafts of xenogeneic embryonic neural tissue in adult rats

Duan, W M LU ; Brundin, P LU ; Grasbon-Frodl, Eva Maria and Widner, H LU (1996) In Brain Research 712(2). p.199-212
Abstract

We studied the effects of high-dose methylprednisolone on the survival of intrastriatal neural xenografts and the host responses against them. Dissociated mesencephalic tissue from inbred mouse (CBA-strain) embryos was transplanted to the intact striatum of adult Sprague-Dawley rats. The rats received either daily injections of methylprednisolone (30 mg/kg), or cyclosporin A (10 mg/kg), or no immunosuppressive treatment. Two or six weeks after transplantation, there was good survival of xenografts in both the methylprednisolone- and cyclosporin A-treated rats. In contrast, the xenografts in untreated control rats were all rejected by six weeks. There was no marked difference in the degree of expression of MHC class I and II antigens and... (More)

We studied the effects of high-dose methylprednisolone on the survival of intrastriatal neural xenografts and the host responses against them. Dissociated mesencephalic tissue from inbred mouse (CBA-strain) embryos was transplanted to the intact striatum of adult Sprague-Dawley rats. The rats received either daily injections of methylprednisolone (30 mg/kg), or cyclosporin A (10 mg/kg), or no immunosuppressive treatment. Two or six weeks after transplantation, there was good survival of xenografts in both the methylprednisolone- and cyclosporin A-treated rats. In contrast, the xenografts in untreated control rats were all rejected by six weeks. There was no marked difference in the degree of expression of MHC class I and II antigens and the accumulation of activated astrocytes and microglial cells/macrophages between the three groups. However, both methylprednisolone and cyclosporin A reduced infiltration of T lymphocytes to the transplantation sites. The expression of pro-inflammatory cytokines (interferon-gamma, tumour necrosis factor-alpha, interleukin-6) in and around the grafts was lower in the methylprednisolone- and cyclosporin A-treated groups than in untreated control rats. Although high-dose methylprednisolone caused significant body weight loss, we conclude that this treatment can prevent rejection of intrastriatal grafts of xenogeneic embryonic neural tissue in the adult.

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keywords
Animals, Brain Chemistry, Brain Tissue Transplantation, CD4-CD8 Ratio, Cytokines, Female, Fetal Tissue Transplantation, Glial Fibrillary Acidic Protein, Graft Rejection, Graft Survival, Immunohistochemistry, Immunosuppressive Agents, Major Histocompatibility Complex, Methylprednisolone, Mice, Neostriatum, Pregnancy, Rats, Rats, Sprague-Dawley, Receptors, Complement, Transplantation, Heterologous, Tyrosine 3-Monooxygenase
in
Brain Research
volume
712
issue
2
pages
14 pages
publisher
Elsevier
external identifiers
  • scopus:0029939038
ISSN
0006-8993
DOI
10.1016/0006-8993(95)01409-8
language
English
LU publication?
yes
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ad11a330-5bef-4a20-947f-49e54c1ca18e
date added to LUP
2017-04-19 18:26:39
date last changed
2017-04-28 12:47:52
@article{ad11a330-5bef-4a20-947f-49e54c1ca18e,
  abstract     = {<p>We studied the effects of high-dose methylprednisolone on the survival of intrastriatal neural xenografts and the host responses against them. Dissociated mesencephalic tissue from inbred mouse (CBA-strain) embryos was transplanted to the intact striatum of adult Sprague-Dawley rats. The rats received either daily injections of methylprednisolone (30 mg/kg), or cyclosporin A (10 mg/kg), or no immunosuppressive treatment. Two or six weeks after transplantation, there was good survival of xenografts in both the methylprednisolone- and cyclosporin A-treated rats. In contrast, the xenografts in untreated control rats were all rejected by six weeks. There was no marked difference in the degree of expression of MHC class I and II antigens and the accumulation of activated astrocytes and microglial cells/macrophages between the three groups. However, both methylprednisolone and cyclosporin A reduced infiltration of T lymphocytes to the transplantation sites. The expression of pro-inflammatory cytokines (interferon-gamma, tumour necrosis factor-alpha, interleukin-6) in and around the grafts was lower in the methylprednisolone- and cyclosporin A-treated groups than in untreated control rats. Although high-dose methylprednisolone caused significant body weight loss, we conclude that this treatment can prevent rejection of intrastriatal grafts of xenogeneic embryonic neural tissue in the adult.</p>},
  author       = {Duan, W M and Brundin, P and Grasbon-Frodl, Eva Maria and Widner, H},
  issn         = {0006-8993},
  keyword      = {Animals,Brain Chemistry,Brain Tissue Transplantation,CD4-CD8 Ratio,Cytokines,Female,Fetal Tissue Transplantation,Glial Fibrillary Acidic Protein,Graft Rejection,Graft Survival,Immunohistochemistry,Immunosuppressive Agents,Major Histocompatibility Complex,Methylprednisolone,Mice,Neostriatum,Pregnancy,Rats,Rats, Sprague-Dawley,Receptors, Complement,Transplantation, Heterologous,Tyrosine 3-Monooxygenase},
  language     = {eng},
  month        = {03},
  number       = {2},
  pages        = {199--212},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Methylprednisolone prevents rejection of intrastriatal grafts of xenogeneic embryonic neural tissue in adult rats},
  url          = {http://dx.doi.org/10.1016/0006-8993(95)01409-8},
  volume       = {712},
  year         = {1996},
}