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Methylprednisolone prevents rejection of intrastriatal grafts of xenogeneic embryonic neural tissue in adult rats

Duan, W M LU ; Brundin, P LU ; Grasbon-Frodl, Eva Maria and Widner, H LU (1996) In Brain Research 712(2). p.199-212
Abstract

We studied the effects of high-dose methylprednisolone on the survival of intrastriatal neural xenografts and the host responses against them. Dissociated mesencephalic tissue from inbred mouse (CBA-strain) embryos was transplanted to the intact striatum of adult Sprague-Dawley rats. The rats received either daily injections of methylprednisolone (30 mg/kg), or cyclosporin A (10 mg/kg), or no immunosuppressive treatment. Two or six weeks after transplantation, there was good survival of xenografts in both the methylprednisolone- and cyclosporin A-treated rats. In contrast, the xenografts in untreated control rats were all rejected by six weeks. There was no marked difference in the degree of expression of MHC class I and II antigens and... (More)

We studied the effects of high-dose methylprednisolone on the survival of intrastriatal neural xenografts and the host responses against them. Dissociated mesencephalic tissue from inbred mouse (CBA-strain) embryos was transplanted to the intact striatum of adult Sprague-Dawley rats. The rats received either daily injections of methylprednisolone (30 mg/kg), or cyclosporin A (10 mg/kg), or no immunosuppressive treatment. Two or six weeks after transplantation, there was good survival of xenografts in both the methylprednisolone- and cyclosporin A-treated rats. In contrast, the xenografts in untreated control rats were all rejected by six weeks. There was no marked difference in the degree of expression of MHC class I and II antigens and the accumulation of activated astrocytes and microglial cells/macrophages between the three groups. However, both methylprednisolone and cyclosporin A reduced infiltration of T lymphocytes to the transplantation sites. The expression of pro-inflammatory cytokines (interferon-gamma, tumour necrosis factor-alpha, interleukin-6) in and around the grafts was lower in the methylprednisolone- and cyclosporin A-treated groups than in untreated control rats. Although high-dose methylprednisolone caused significant body weight loss, we conclude that this treatment can prevent rejection of intrastriatal grafts of xenogeneic embryonic neural tissue in the adult.

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type
Contribution to journal
publication status
published
subject
keywords
Animals, Brain Chemistry, Brain Tissue Transplantation, CD4-CD8 Ratio, Cytokines, Female, Fetal Tissue Transplantation, Glial Fibrillary Acidic Protein, Graft Rejection, Graft Survival, Immunohistochemistry, Immunosuppressive Agents, Major Histocompatibility Complex, Methylprednisolone, Mice, Neostriatum, Pregnancy, Rats, Rats, Sprague-Dawley, Receptors, Complement, Transplantation, Heterologous, Tyrosine 3-Monooxygenase
in
Brain Research
volume
712
issue
2
pages
14 pages
publisher
Elsevier
external identifiers
  • pmid:8814894
  • scopus:0029939038
ISSN
0006-8993
DOI
10.1016/0006-8993(95)01409-8
language
English
LU publication?
yes
id
ad11a330-5bef-4a20-947f-49e54c1ca18e
date added to LUP
2017-04-19 18:26:39
date last changed
2025-01-07 11:41:19
@article{ad11a330-5bef-4a20-947f-49e54c1ca18e,
  abstract     = {{<p>We studied the effects of high-dose methylprednisolone on the survival of intrastriatal neural xenografts and the host responses against them. Dissociated mesencephalic tissue from inbred mouse (CBA-strain) embryos was transplanted to the intact striatum of adult Sprague-Dawley rats. The rats received either daily injections of methylprednisolone (30 mg/kg), or cyclosporin A (10 mg/kg), or no immunosuppressive treatment. Two or six weeks after transplantation, there was good survival of xenografts in both the methylprednisolone- and cyclosporin A-treated rats. In contrast, the xenografts in untreated control rats were all rejected by six weeks. There was no marked difference in the degree of expression of MHC class I and II antigens and the accumulation of activated astrocytes and microglial cells/macrophages between the three groups. However, both methylprednisolone and cyclosporin A reduced infiltration of T lymphocytes to the transplantation sites. The expression of pro-inflammatory cytokines (interferon-gamma, tumour necrosis factor-alpha, interleukin-6) in and around the grafts was lower in the methylprednisolone- and cyclosporin A-treated groups than in untreated control rats. Although high-dose methylprednisolone caused significant body weight loss, we conclude that this treatment can prevent rejection of intrastriatal grafts of xenogeneic embryonic neural tissue in the adult.</p>}},
  author       = {{Duan, W M and Brundin, P and Grasbon-Frodl, Eva Maria and Widner, H}},
  issn         = {{0006-8993}},
  keywords     = {{Animals; Brain Chemistry; Brain Tissue Transplantation; CD4-CD8 Ratio; Cytokines; Female; Fetal Tissue Transplantation; Glial Fibrillary Acidic Protein; Graft Rejection; Graft Survival; Immunohistochemistry; Immunosuppressive Agents; Major Histocompatibility Complex; Methylprednisolone; Mice; Neostriatum; Pregnancy; Rats; Rats, Sprague-Dawley; Receptors, Complement; Transplantation, Heterologous; Tyrosine 3-Monooxygenase}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{2}},
  pages        = {{199--212}},
  publisher    = {{Elsevier}},
  series       = {{Brain Research}},
  title        = {{Methylprednisolone prevents rejection of intrastriatal grafts of xenogeneic embryonic neural tissue in adult rats}},
  url          = {{http://dx.doi.org/10.1016/0006-8993(95)01409-8}},
  doi          = {{10.1016/0006-8993(95)01409-8}},
  volume       = {{712}},
  year         = {{1996}},
}