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Oral Administration of Lactobacillus plantarum 299v Reduces Cortisol Levels in Human Saliva during Examination Induced Stress : A Randomized, Double-Blind Controlled Trial

Andersson, Hannah LU ; Tullberg, Cecilia LU ; Ahrné, Siv LU ; Hamberg, Kristina ; Lazou Ahrén, Irini LU ; Molin, Göran LU ; Sonesson, Mikael and Håkansson, Åsa LU (2016) In International Journal of Microbiology 2016.
Abstract

Objective. To clarify the effect of Lactobacillus plantarum 299v on the salivary cortisol and salivary IgA levels in young adults under examination stress. Design. Forty-one students with an upcoming academic exam were included in a randomized double-blind, placebo-controlled study. The probiotic bacteria or the placebo product was administered in capsules once a day during 14 days. Saliva was collected and a perceived stress test was filled out at each sampling occasion. Saliva was collected for cortisol analysis by Electrochemiluminescence Immunoassay (ECLI) and salivary IgA was analysed by Enzyme-Linked Immunosorbent Assay (ELISA). Abundance of lactobacilli was evaluated by cultivation of saliva on selective medium and identification... (More)

Objective. To clarify the effect of Lactobacillus plantarum 299v on the salivary cortisol and salivary IgA levels in young adults under examination stress. Design. Forty-one students with an upcoming academic exam were included in a randomized double-blind, placebo-controlled study. The probiotic bacteria or the placebo product was administered in capsules once a day during 14 days. Saliva was collected and a perceived stress test was filled out at each sampling occasion. Saliva was collected for cortisol analysis by Electrochemiluminescence Immunoassay (ECLI) and salivary IgA was analysed by Enzyme-Linked Immunosorbent Assay (ELISA). Abundance of lactobacilli was evaluated by cultivation of saliva on selective medium and identification of L. plantarum 299v was done on randomly selected colonies by a random amplification of polymorphic DNA (RAPD) typing. Results. A significant difference in cortisol levels was found between the treatment group and the placebo group (P < 0.05), together with a significant increase in levels of lactobacilli in the treatment group compared with the placebo group (P < 0.001). No significant changes were found for salivary IgA. Conclusion. A probiotic bacterium with ability to reduce symptoms of irritable bowel syndrome (IBS) prohibited increased levels of the stress marker cortisol during the examination period. The registration number of the study is NCT02974894, and the study is registered at ClinicalTrials.gov.

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publication status
published
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in
International Journal of Microbiology
volume
2016
pages
7 pages
publisher
Hindawi Limited
external identifiers
  • pmid:28101105
  • scopus:85008700665
ISSN
1687-918X
DOI
10.1155/2016/8469018
language
English
LU publication?
yes
id
aed0a588-b7aa-46e3-a75d-dfe12d81635b
date added to LUP
2019-07-01 16:25:45
date last changed
2024-06-27 01:10:58
@article{aed0a588-b7aa-46e3-a75d-dfe12d81635b,
  abstract     = {{<p>Objective. To clarify the effect of Lactobacillus plantarum 299v on the salivary cortisol and salivary IgA levels in young adults under examination stress. Design. Forty-one students with an upcoming academic exam were included in a randomized double-blind, placebo-controlled study. The probiotic bacteria or the placebo product was administered in capsules once a day during 14 days. Saliva was collected and a perceived stress test was filled out at each sampling occasion. Saliva was collected for cortisol analysis by Electrochemiluminescence Immunoassay (ECLI) and salivary IgA was analysed by Enzyme-Linked Immunosorbent Assay (ELISA). Abundance of lactobacilli was evaluated by cultivation of saliva on selective medium and identification of L. plantarum 299v was done on randomly selected colonies by a random amplification of polymorphic DNA (RAPD) typing. Results. A significant difference in cortisol levels was found between the treatment group and the placebo group (P &lt; 0.05), together with a significant increase in levels of lactobacilli in the treatment group compared with the placebo group (P &lt; 0.001). No significant changes were found for salivary IgA. Conclusion. A probiotic bacterium with ability to reduce symptoms of irritable bowel syndrome (IBS) prohibited increased levels of the stress marker cortisol during the examination period. The registration number of the study is NCT02974894, and the study is registered at ClinicalTrials.gov.</p>}},
  author       = {{Andersson, Hannah and Tullberg, Cecilia and Ahrné, Siv and Hamberg, Kristina and Lazou Ahrén, Irini and Molin, Göran and Sonesson, Mikael and Håkansson, Åsa}},
  issn         = {{1687-918X}},
  language     = {{eng}},
  publisher    = {{Hindawi Limited}},
  series       = {{International Journal of Microbiology}},
  title        = {{Oral Administration of Lactobacillus plantarum 299v Reduces Cortisol Levels in Human Saliva during Examination Induced Stress : A Randomized, Double-Blind Controlled Trial}},
  url          = {{http://dx.doi.org/10.1155/2016/8469018}},
  doi          = {{10.1155/2016/8469018}},
  volume       = {{2016}},
  year         = {{2016}},
}