Cardiovascular Disease-Related Serum Proteins in Workers Occupationally Exposed to Polycyclic Aromatic Hydrocarbons
(2019) In Toxicological Sciences 171(1). p.235-246- Abstract
Chimney sweeps have higher incidence and mortality of cardiovascular disease (CVD), likely related to their exposure to polycyclic aromatic hydrocarbons (PAH). In order to identify underlying mechanisms of PAH-related CVD, we here investigated whether PAH exposure was associated with levels of putative CVD-related proteins in serum among currently working chimney sweeps. We enrolled 116 chimney sweeps and 125 unexposed controls, all nonsmoking male workers from Sweden. We measured monohydroxylated PAH metabolites in urine by liquid chromatography coupled to tandem mass spectrometry and a panel of 85 proteins in serum using proximity extension assay. Linear regression analysis adjusted for age and body mass index showed that 25 proteins... (More)
Chimney sweeps have higher incidence and mortality of cardiovascular disease (CVD), likely related to their exposure to polycyclic aromatic hydrocarbons (PAH). In order to identify underlying mechanisms of PAH-related CVD, we here investigated whether PAH exposure was associated with levels of putative CVD-related proteins in serum among currently working chimney sweeps. We enrolled 116 chimney sweeps and 125 unexposed controls, all nonsmoking male workers from Sweden. We measured monohydroxylated PAH metabolites in urine by liquid chromatography coupled to tandem mass spectrometry and a panel of 85 proteins in serum using proximity extension assay. Linear regression analysis adjusted for age and body mass index showed that 25 proteins were differentially expressed between chimney sweeps and the controls (p <. 05, adjusted for false discovery rate). Of the 25 proteins, follistatin (FS), prointerleukin-16 (IL-16), and heat shock protein beta-1 (HSP 27) showed positive associations with the monohydroxylated metabolites of PAH in a dose-response manner (p <. 05). Pathway and gene ontology analyses demonstrated that the differentially expressed proteins were mainly involved in inflammatory response and immunological functions, such as leukocyte migration, cell movement of leukocytes, and adhesion of immune cells. In conclusion, we found a number of putative CVD-related proteins differentially expressed, between PAH-exposed and unexposed individuals, and mainly involved in inflammation and immune function. Our data warrant protective measures to reduce PAH exposure and longitudinal investigations of the protein profile in chimney sweeps and other occupational groups exposed to PAH.
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- author
- Alhamdow, Ayman LU ; Lindh, Christian LU ; Albin, Maria LU ; Gustavsson, Per LU ; Tinnerberg, Håkan LU and Broberg, Karin LU
- organization
- publishing date
- 2019-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- biomarkers, chimney sweeps, circulating, inflammation, PAH metabolites, work-related
- in
- Toxicological Sciences
- volume
- 171
- issue
- 1
- pages
- 235 - 246
- publisher
- Oxford University Press
- external identifiers
-
- pmid:31228248
- scopus:85076013742
- ISSN
- 1096-6080
- DOI
- 10.1093/toxsci/kfz142
- language
- English
- LU publication?
- yes
- additional info
- Funding Information: This study was financially supported by the Swedish Research Council for Health, Working Life and Welfare (FORTE; grant number 2012-00402), AFA Insurance (AFA Försa€kring; grant number 120115), and Karolinska Institutet. Publisher Copyright: © 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the Society of Toxicology.
- id
- b07ceb09-e26c-45ca-ad8e-073ba53047bb
- date added to LUP
- 2023-09-26 10:30:22
- date last changed
- 2024-03-07 01:15:24
@article{b07ceb09-e26c-45ca-ad8e-073ba53047bb, abstract = {{<p>Chimney sweeps have higher incidence and mortality of cardiovascular disease (CVD), likely related to their exposure to polycyclic aromatic hydrocarbons (PAH). In order to identify underlying mechanisms of PAH-related CVD, we here investigated whether PAH exposure was associated with levels of putative CVD-related proteins in serum among currently working chimney sweeps. We enrolled 116 chimney sweeps and 125 unexposed controls, all nonsmoking male workers from Sweden. We measured monohydroxylated PAH metabolites in urine by liquid chromatography coupled to tandem mass spectrometry and a panel of 85 proteins in serum using proximity extension assay. Linear regression analysis adjusted for age and body mass index showed that 25 proteins were differentially expressed between chimney sweeps and the controls (p <. 05, adjusted for false discovery rate). Of the 25 proteins, follistatin (FS), prointerleukin-16 (IL-16), and heat shock protein beta-1 (HSP 27) showed positive associations with the monohydroxylated metabolites of PAH in a dose-response manner (p <. 05). Pathway and gene ontology analyses demonstrated that the differentially expressed proteins were mainly involved in inflammatory response and immunological functions, such as leukocyte migration, cell movement of leukocytes, and adhesion of immune cells. In conclusion, we found a number of putative CVD-related proteins differentially expressed, between PAH-exposed and unexposed individuals, and mainly involved in inflammation and immune function. Our data warrant protective measures to reduce PAH exposure and longitudinal investigations of the protein profile in chimney sweeps and other occupational groups exposed to PAH.</p>}}, author = {{Alhamdow, Ayman and Lindh, Christian and Albin, Maria and Gustavsson, Per and Tinnerberg, Håkan and Broberg, Karin}}, issn = {{1096-6080}}, keywords = {{biomarkers; chimney sweeps; circulating; inflammation; PAH metabolites; work-related}}, language = {{eng}}, month = {{09}}, number = {{1}}, pages = {{235--246}}, publisher = {{Oxford University Press}}, series = {{Toxicological Sciences}}, title = {{Cardiovascular Disease-Related Serum Proteins in Workers Occupationally Exposed to Polycyclic Aromatic Hydrocarbons}}, url = {{http://dx.doi.org/10.1093/toxsci/kfz142}}, doi = {{10.1093/toxsci/kfz142}}, volume = {{171}}, year = {{2019}}, }