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Impact of diet-induced obesity on the mouse brain phosphoproteome

Siino, Valentina LU ; Amato, Antonella; Di Salvo, Francesca; Caldara, Gaetano Felice; Filogamo, Marcello; James, Peter LU and Vasto, Sonya (2018) In Journal of Nutritional Biochemistry 58. p.102-109
Abstract

Obesity is closely associated to several diseases such as type 2 diabetes, cardiovascular disease, hepatic steatosis, airway disease, neurodegeneration, biliary diseases and certain cancers. It is, therefore, of importance to assess the role of nutrition in disease prevention as well as its effect in the course of such pathologies. In the present study, we addressed the impact of the exposure to different obesogenic diets in the mice brains phosphoproteome. To analyze if the obesity could be able to modify the protein pattern expression of brain neurons, obesity was induced in two different groups of mice. One group of mice was fed with hyperglycemic diet (HGD) and the other one was fed with high-fat diet (HFD), both for 12 weeks. A... (More)

Obesity is closely associated to several diseases such as type 2 diabetes, cardiovascular disease, hepatic steatosis, airway disease, neurodegeneration, biliary diseases and certain cancers. It is, therefore, of importance to assess the role of nutrition in disease prevention as well as its effect in the course of such pathologies. In the present study, we addressed the impact of the exposure to different obesogenic diets in the mice brains phosphoproteome. To analyze if the obesity could be able to modify the protein pattern expression of brain neurons, obesity was induced in two different groups of mice. One group of mice was fed with hyperglycemic diet (HGD) and the other one was fed with high-fat diet (HFD), both for 12 weeks. A control group of lean mice was fed with a standard diet (SD). Metabolic parameters were measured before sacrifice, and brains were harvested for label-free phosphoproteomic analysis. Mice brains were analyzed to find differences, if any, in protein phosphorylation. Interestingly, the changes were independent of the obesogenic diet as no changes were detected between the two obese groups. Dephosphorylation of proteins involved in neuronal development (among others SYNGAP1 and PPP1R9B), in vesicle trafficking (for example SNAP91 and AMPH) and in cytoskeletal functions (for example, CLASP2 and GSK3B) was identified, while increased phosphorylation was detected for microtubule proteins (such as MAP2 and MAPT). Phospho site analysis of the mouse brain proteome reveals important changes that point to a connection between diet-induced obesity and impairment of neuronal functions and signaling.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
High-fat diet, Hyperglycemic diet, Neuronal impairment, Nutrition, Obesity, Phosphoproteomics
in
Journal of Nutritional Biochemistry
volume
58
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85048206666
ISSN
0955-2863
DOI
10.1016/j.jnutbio.2018.04.015
language
English
LU publication?
yes
id
b16d1850-55f5-4ab7-a803-a0e7e0efb0b6
date added to LUP
2018-06-20 15:00:34
date last changed
2019-02-20 11:20:31
@article{b16d1850-55f5-4ab7-a803-a0e7e0efb0b6,
  abstract     = {<p>Obesity is closely associated to several diseases such as type 2 diabetes, cardiovascular disease, hepatic steatosis, airway disease, neurodegeneration, biliary diseases and certain cancers. It is, therefore, of importance to assess the role of nutrition in disease prevention as well as its effect in the course of such pathologies. In the present study, we addressed the impact of the exposure to different obesogenic diets in the mice brains phosphoproteome. To analyze if the obesity could be able to modify the protein pattern expression of brain neurons, obesity was induced in two different groups of mice. One group of mice was fed with hyperglycemic diet (HGD) and the other one was fed with high-fat diet (HFD), both for 12 weeks. A control group of lean mice was fed with a standard diet (SD). Metabolic parameters were measured before sacrifice, and brains were harvested for label-free phosphoproteomic analysis. Mice brains were analyzed to find differences, if any, in protein phosphorylation. Interestingly, the changes were independent of the obesogenic diet as no changes were detected between the two obese groups. Dephosphorylation of proteins involved in neuronal development (among others SYNGAP1 and PPP1R9B), in vesicle trafficking (for example SNAP91 and AMPH) and in cytoskeletal functions (for example, CLASP2 and GSK3B) was identified, while increased phosphorylation was detected for microtubule proteins (such as MAP2 and MAPT). Phospho site analysis of the mouse brain proteome reveals important changes that point to a connection between diet-induced obesity and impairment of neuronal functions and signaling.</p>},
  author       = {Siino, Valentina and Amato, Antonella and Di Salvo, Francesca and Caldara, Gaetano Felice and Filogamo, Marcello and James, Peter and Vasto, Sonya},
  issn         = {0955-2863},
  keyword      = {High-fat diet,Hyperglycemic diet,Neuronal impairment,Nutrition,Obesity,Phosphoproteomics},
  language     = {eng},
  month        = {08},
  pages        = {102--109},
  publisher    = {Elsevier},
  series       = {Journal of Nutritional Biochemistry},
  title        = {Impact of diet-induced obesity on the mouse brain phosphoproteome},
  url          = {http://dx.doi.org/10.1016/j.jnutbio.2018.04.015},
  volume       = {58},
  year         = {2018},
}