Effects of extracellular calcium and magnesium on bile salt stimulated amylase release from rat pancreatic acini
(1986) In Scandinavian Journal of Gastroenterology 21(10). p.1211-1216- Abstract
- The effects of extracellular calcium and magnesium on bile-salt-induced amylase release from rat pancreatic acini have been studied. The amylase releases caused by from 0.25 mM to 1.0 mM taurodeoxycholate (TDC) and by taurochenodeoxycholate (TCDC) at a concentration of more than 0.75 mM were reduced by ethylenediaminetetraacetic acid (EDTA) and increased by verapamil. EDTA and verapamil had no significant effect on the taurocholate (TC) (1.0-5.0 mM)-stimulated amylase release. The inhibiting effect of EDTA began to appear already during the initial 5 min and was not parallel to any change of lactate dehydrogenase release. The TDC- and TCDC-stimulated amylase release was strongly dependent on the concentrations of extracellular calcium and... (More)
- The effects of extracellular calcium and magnesium on bile-salt-induced amylase release from rat pancreatic acini have been studied. The amylase releases caused by from 0.25 mM to 1.0 mM taurodeoxycholate (TDC) and by taurochenodeoxycholate (TCDC) at a concentration of more than 0.75 mM were reduced by ethylenediaminetetraacetic acid (EDTA) and increased by verapamil. EDTA and verapamil had no significant effect on the taurocholate (TC) (1.0-5.0 mM)-stimulated amylase release. The inhibiting effect of EDTA began to appear already during the initial 5 min and was not parallel to any change of lactate dehydrogenase release. The TDC- and TCDC-stimulated amylase release was strongly dependent on the concentrations of extracellular calcium and was only weakly dependent on extracellular magnesium. The TC-induced amylase release was slightly increased only at high concentrations of calcium and magnesium. It is suggested that the mechanism of dihydroxy bile-salt-induced amylase release from rat pancreatic acini is different from that of trihydroxy bile salt. The dihydroxy bile-salt-stimulated amylase release is dependent on extracellular calcium but does not seem to be related to the uptake of calcium by the acini. (Less)
Please use this url to cite or link to this publication:
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- author
- Duan, Rui-Dong LU and Erlanson-Albertsson, Charlotte LU
- organization
- publishing date
- 1986
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Gastroenterology
- volume
- 21
- issue
- 10
- pages
- 1211 - 1216
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0023009703
- ISSN
- 0036-5521
- DOI
- 10.3109/00365528608996445
- language
- English
- LU publication?
- yes
- id
- b20a1de6-c83b-4715-b4e5-d87bfb0cc5ad
- date added to LUP
- 2019-02-02 16:27:18
- date last changed
- 2024-01-15 13:31:42
@article{b20a1de6-c83b-4715-b4e5-d87bfb0cc5ad, abstract = {{The effects of extracellular calcium and magnesium on bile-salt-induced amylase release from rat pancreatic acini have been studied. The amylase releases caused by from 0.25 mM to 1.0 mM taurodeoxycholate (TDC) and by taurochenodeoxycholate (TCDC) at a concentration of more than 0.75 mM were reduced by ethylenediaminetetraacetic acid (EDTA) and increased by verapamil. EDTA and verapamil had no significant effect on the taurocholate (TC) (1.0-5.0 mM)-stimulated amylase release. The inhibiting effect of EDTA began to appear already during the initial 5 min and was not parallel to any change of lactate dehydrogenase release. The TDC- and TCDC-stimulated amylase release was strongly dependent on the concentrations of extracellular calcium and was only weakly dependent on extracellular magnesium. The TC-induced amylase release was slightly increased only at high concentrations of calcium and magnesium. It is suggested that the mechanism of dihydroxy bile-salt-induced amylase release from rat pancreatic acini is different from that of trihydroxy bile salt. The dihydroxy bile-salt-stimulated amylase release is dependent on extracellular calcium but does not seem to be related to the uptake of calcium by the acini.}}, author = {{Duan, Rui-Dong and Erlanson-Albertsson, Charlotte}}, issn = {{0036-5521}}, language = {{eng}}, number = {{10}}, pages = {{1211--1216}}, publisher = {{Taylor & Francis}}, series = {{Scandinavian Journal of Gastroenterology}}, title = {{Effects of extracellular calcium and magnesium on bile salt stimulated amylase release from rat pancreatic acini}}, url = {{http://dx.doi.org/10.3109/00365528608996445}}, doi = {{10.3109/00365528608996445}}, volume = {{21}}, year = {{1986}}, }