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Aqueous polymer two-phase systems and their use in fragmentation and separation of biological membranes for the purpose of mapping the membrane structure.

Danielsson, Ravi LU and Albertsson, Per-Åke LU (2013) In Preparative Biochemistry & Biotechnology 43(5). p.512-525
Abstract
When solutions of two different polymers are mixed, phase separation often occurs even at low concentrations of polymers. One polymer usually collects in one phase and the other polymer in the other phase. When water is used as solvent, two aqueous, immiscible, phases are obtained. The same holds for aqueous mixtures of a salt and a polymer. Such aqueous two-phase systems (ATPS) are very useful for separation of high-molecular-weight biomolecules such as proteins and nucleic acids and also for cells, cell organelles, and membrane vesicles. The phase systems can be made highly selective and they are also mild toward biomolecules and cell particles. In this review we describe how ATPS can be used for fragmentation and separation analyses of... (More)
When solutions of two different polymers are mixed, phase separation often occurs even at low concentrations of polymers. One polymer usually collects in one phase and the other polymer in the other phase. When water is used as solvent, two aqueous, immiscible, phases are obtained. The same holds for aqueous mixtures of a salt and a polymer. Such aqueous two-phase systems (ATPS) are very useful for separation of high-molecular-weight biomolecules such as proteins and nucleic acids and also for cells, cell organelles, and membrane vesicles. The phase systems can be made highly selective and they are also mild toward biomolecules and cell particles. In this review we describe how ATPS can be used for fragmentation and separation analyses of biological membranes and how this can be used for mapping of the photosynthetic membrane, the thylakoid, of green leaves. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Preparative Biochemistry & Biotechnology
volume
43
issue
5
pages
512 - 525
publisher
Taylor & Francis
external identifiers
  • wos:000317823500008
  • pmid:23581785
  • scopus:84876368483
  • pmid:23581785
ISSN
1532-2297
DOI
10.1080/10826068.2013.773449
language
English
LU publication?
yes
id
b3742242-2e08-4127-80af-c887daced486 (old id 3733815)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23581785?dopt=Abstract
date added to LUP
2016-04-01 09:51:31
date last changed
2022-01-25 17:24:16
@article{b3742242-2e08-4127-80af-c887daced486,
  abstract     = {{When solutions of two different polymers are mixed, phase separation often occurs even at low concentrations of polymers. One polymer usually collects in one phase and the other polymer in the other phase. When water is used as solvent, two aqueous, immiscible, phases are obtained. The same holds for aqueous mixtures of a salt and a polymer. Such aqueous two-phase systems (ATPS) are very useful for separation of high-molecular-weight biomolecules such as proteins and nucleic acids and also for cells, cell organelles, and membrane vesicles. The phase systems can be made highly selective and they are also mild toward biomolecules and cell particles. In this review we describe how ATPS can be used for fragmentation and separation analyses of biological membranes and how this can be used for mapping of the photosynthetic membrane, the thylakoid, of green leaves.}},
  author       = {{Danielsson, Ravi and Albertsson, Per-Åke}},
  issn         = {{1532-2297}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{512--525}},
  publisher    = {{Taylor & Francis}},
  series       = {{Preparative Biochemistry & Biotechnology}},
  title        = {{Aqueous polymer two-phase systems and their use in fragmentation and separation of biological membranes for the purpose of mapping the membrane structure.}},
  url          = {{http://dx.doi.org/10.1080/10826068.2013.773449}},
  doi          = {{10.1080/10826068.2013.773449}},
  volume       = {{43}},
  year         = {{2013}},
}