Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Tissue microarray based analysis of prognostic markers in invasive bladder cancer: Much effort to no avail?

Liedberg, Fredrik LU ; Anderson, Harald LU ; Chebil, G ; Fernö, Mårten LU ; Gudjonsson, Sigurdur LU ; Höglund, Mattias LU ; Lindgren, David LU ; Lundberg, Lena-Maria LU ; Lövgren, Kristina LU and Månsson, Wiking LU (2008) In Urologic Oncology 26(1). p.17-24
Abstract
PURPOSE: To evaluate altered protein expression with tissue microarray methodology for 15 different markers with potential prognostic significance in invasive bladder cancer. MATERIALS AND METHODS: Invasive tumor was sampled with the tissue-arraying instrument in 133 consecutive patients who underwent radical cystectomy, and at least 3, 0.6-mm tissue cores were obtained. With immunohistochemistry, the expressions of TP53, RB1, CDKN1A (p21), MKI67 (Ki67), PTGS2 (Cox-2), CTNNA1 (alpha-catenin), CTNNB1 (beta-catenin), AKT, PTEN, RHOA, RHOC, STAT1, VEGFC, EGFR, and ERBB2 (HER2) were quantified, and correlations were made with tumor grade, pathologic stage, lymph node status, and disease-specific survival. RESULTS: Decreased immunohistochemical... (More)
PURPOSE: To evaluate altered protein expression with tissue microarray methodology for 15 different markers with potential prognostic significance in invasive bladder cancer. MATERIALS AND METHODS: Invasive tumor was sampled with the tissue-arraying instrument in 133 consecutive patients who underwent radical cystectomy, and at least 3, 0.6-mm tissue cores were obtained. With immunohistochemistry, the expressions of TP53, RB1, CDKN1A (p21), MKI67 (Ki67), PTGS2 (Cox-2), CTNNA1 (alpha-catenin), CTNNB1 (beta-catenin), AKT, PTEN, RHOA, RHOC, STAT1, VEGFC, EGFR, and ERBB2 (HER2) were quantified, and correlations were made with tumor grade, pathologic stage, lymph node status, and disease-specific survival. RESULTS: Decreased immunohistochemical expression of CTNNA1 and of PTEN correlated with higher pathologic tumor stages (P = 0.01 and P = 0.01, respectively), whereas increased AKT1 and ERBB2 correlated with lower pathologic tumor stages (P = 0.01 and P = 0.03, respectively). Increased RHOA expression was more common in grade 3 than in grade 2 tumors (P = 0.016). There were no other correlations among the 15 factors studied and pathologic stage, lymph node status, or tumor grade. No association was found between bladder cancer death and altered marker status for any of the markers studied. CONCLUSIONS: Currently, there are reasons to have a skeptical attitude toward the value of tissue microarray based immunohistochemistry as a method for evaluating prognostic markers in invasive bladder cancer. In this study, 15 antibodies were tested but were found to be of little clinical value. Whether this negative finding is related to the group of patients or factors studied, or the methodology is unclear. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Urologic Oncology
volume
26
issue
1
pages
17 - 24
publisher
Elsevier
external identifiers
  • scopus:37649024111
  • pmid:18190825
  • wos:000252680100003
  • pmid:18190825
ISSN
1873-2496
DOI
10.1016/j.urolonc.2006.08.021
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Oncology, MV (013035000), Pathology, (Lund) (013030000), Division of Clinical Genetics (013022003), Department of Urology, Lund (013077000), Cancer Epidemiology (013007100)
id
b7988f4c-fa0d-4266-b6ca-562232798ae4 (old id 1142817)
date added to LUP
2016-04-01 11:56:14
date last changed
2022-02-03 07:14:03
@article{b7988f4c-fa0d-4266-b6ca-562232798ae4,
  abstract     = {{PURPOSE: To evaluate altered protein expression with tissue microarray methodology for 15 different markers with potential prognostic significance in invasive bladder cancer. MATERIALS AND METHODS: Invasive tumor was sampled with the tissue-arraying instrument in 133 consecutive patients who underwent radical cystectomy, and at least 3, 0.6-mm tissue cores were obtained. With immunohistochemistry, the expressions of TP53, RB1, CDKN1A (p21), MKI67 (Ki67), PTGS2 (Cox-2), CTNNA1 (alpha-catenin), CTNNB1 (beta-catenin), AKT, PTEN, RHOA, RHOC, STAT1, VEGFC, EGFR, and ERBB2 (HER2) were quantified, and correlations were made with tumor grade, pathologic stage, lymph node status, and disease-specific survival. RESULTS: Decreased immunohistochemical expression of CTNNA1 and of PTEN correlated with higher pathologic tumor stages (P = 0.01 and P = 0.01, respectively), whereas increased AKT1 and ERBB2 correlated with lower pathologic tumor stages (P = 0.01 and P = 0.03, respectively). Increased RHOA expression was more common in grade 3 than in grade 2 tumors (P = 0.016). There were no other correlations among the 15 factors studied and pathologic stage, lymph node status, or tumor grade. No association was found between bladder cancer death and altered marker status for any of the markers studied. CONCLUSIONS: Currently, there are reasons to have a skeptical attitude toward the value of tissue microarray based immunohistochemistry as a method for evaluating prognostic markers in invasive bladder cancer. In this study, 15 antibodies were tested but were found to be of little clinical value. Whether this negative finding is related to the group of patients or factors studied, or the methodology is unclear.}},
  author       = {{Liedberg, Fredrik and Anderson, Harald and Chebil, G and Fernö, Mårten and Gudjonsson, Sigurdur and Höglund, Mattias and Lindgren, David and Lundberg, Lena-Maria and Lövgren, Kristina and Månsson, Wiking}},
  issn         = {{1873-2496}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{17--24}},
  publisher    = {{Elsevier}},
  series       = {{Urologic Oncology}},
  title        = {{Tissue microarray based analysis of prognostic markers in invasive bladder cancer: Much effort to no avail?}},
  url          = {{http://dx.doi.org/10.1016/j.urolonc.2006.08.021}},
  doi          = {{10.1016/j.urolonc.2006.08.021}},
  volume       = {{26}},
  year         = {{2008}},
}