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Distinct phenotypes of platelet, monocyte, and neutrophil activation occur during the acute and convalescent phase of COVID-19

Chao, Yashuan LU ; Rebetz, Johan LU orcid ; Bläckberg, Anna LU ; Hovold, Gisela LU ; Sunnerhagen, Torgny LU orcid ; Rasmussen, Magnus LU ; Semple, John W. LU and Shannon, Oonagh LU (2021) In Platelets
Abstract

SARS-CoV-2 has spread rapidly worldwide, causing the COVID-19 pandemic. Platelet activation and platelet-leukocyte complex formation are proposed to contribute to disease progression. Here, we report platelet and leukocyte activation during acute and convalescent COVID-19 in patients recruited between May-July 2020. Blood samples were analyzed by flow cytometry and ELISA using paired comparison between inclusion (day 0) and 28 days later. The majority of patients were mildly or moderately ill with significantly higher cytokine levels (IL-6 and IL-10) on day 0 as compared with day 28. Platelet activation and granule release were significantly higher on day 0 compared with day 28, as determined by ADP- or thrombin-induced surface CD62P... (More)

SARS-CoV-2 has spread rapidly worldwide, causing the COVID-19 pandemic. Platelet activation and platelet-leukocyte complex formation are proposed to contribute to disease progression. Here, we report platelet and leukocyte activation during acute and convalescent COVID-19 in patients recruited between May-July 2020. Blood samples were analyzed by flow cytometry and ELISA using paired comparison between inclusion (day 0) and 28 days later. The majority of patients were mildly or moderately ill with significantly higher cytokine levels (IL-6 and IL-10) on day 0 as compared with day 28. Platelet activation and granule release were significantly higher on day 0 compared with day 28, as determined by ADP- or thrombin-induced surface CD62P expression, baseline released CD62P, and thrombin-induced platelet-monocyte complex formation. Monocyte activation and procoagulant status at baseline and post activation were heterogeneous but generally lower on day 0 compared with day 28. Baseline and thrombin- or fMLF-induced neutrophil activation and procoagulant status were significantly lower on day 0 compared with day 28. We demonstrate that during the acute phase of COVID-19 compared with the convalescent phase, platelets are more responsive while neutrophils are less responsive. COVID-19 is associated with thromboembolic events where platelet activation and interaction with leukocytes may play an important role.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Blood platelets, COVID-19, monocytes, neutrophils, platelet activation
in
Platelets
publisher
Taylor & Francis
external identifiers
  • pmid:33999778
  • scopus:85106321053
ISSN
0953-7104
DOI
10.1080/09537104.2021.1921721
project
Platelet phenotypic profiling during the progression of COVID-19 disease
language
English
LU publication?
yes
additional info
Funding Information: This work was supported by grants by the Swedish Research Council (Vetenskapsrådet, VR) under grants 2018-05795 (O.S.) and 2017-01779 (J.W.S.); Avtal om Läkarutbildning och Forskning under grant 8439 (J.W.S.); and Avtal om Landstingsanknuten Forskning under grant 2018-0253 (M.R.). We thank the hospital staff for assistance with blood sampling. We thank Professor Mattias Collin (Lund University) for promptly coordinating and establishing a working environment for COVID-19 studies. We thank Fredrik Kahn (Lund University) for important advice regarding the study. Publisher Copyright: © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
id
b8ddd067-d5ef-499c-9df0-2b4e9819accd
date added to LUP
2021-08-08 15:20:59
date last changed
2024-03-23 07:11:30
@article{b8ddd067-d5ef-499c-9df0-2b4e9819accd,
  abstract     = {{<p>SARS-CoV-2 has spread rapidly worldwide, causing the COVID-19 pandemic. Platelet activation and platelet-leukocyte complex formation are proposed to contribute to disease progression. Here, we report platelet and leukocyte activation during acute and convalescent COVID-19 in patients recruited between May-July 2020. Blood samples were analyzed by flow cytometry and ELISA using paired comparison between inclusion (day 0) and 28 days later. The majority of patients were mildly or moderately ill with significantly higher cytokine levels (IL-6 and IL-10) on day 0 as compared with day 28. Platelet activation and granule release were significantly higher on day 0 compared with day 28, as determined by ADP- or thrombin-induced surface CD62P expression, baseline released CD62P, and thrombin-induced platelet-monocyte complex formation. Monocyte activation and procoagulant status at baseline and post activation were heterogeneous but generally lower on day 0 compared with day 28. Baseline and thrombin- or fMLF-induced neutrophil activation and procoagulant status were significantly lower on day 0 compared with day 28. We demonstrate that during the acute phase of COVID-19 compared with the convalescent phase, platelets are more responsive while neutrophils are less responsive. COVID-19 is associated with thromboembolic events where platelet activation and interaction with leukocytes may play an important role.</p>}},
  author       = {{Chao, Yashuan and Rebetz, Johan and Bläckberg, Anna and Hovold, Gisela and Sunnerhagen, Torgny and Rasmussen, Magnus and Semple, John W. and Shannon, Oonagh}},
  issn         = {{0953-7104}},
  keywords     = {{Blood platelets; COVID-19; monocytes; neutrophils; platelet activation}},
  language     = {{eng}},
  publisher    = {{Taylor & Francis}},
  series       = {{Platelets}},
  title        = {{Distinct phenotypes of platelet, monocyte, and neutrophil activation occur during the acute and convalescent phase of COVID-19}},
  url          = {{https://lup.lub.lu.se/search/files/119180223/Distinct_phenotypes_of_platelet_monocyte_and_neutrophil_activation_occur_during_the_acute_and_convalescent_phase_of_COVID_19.pdf}},
  doi          = {{10.1080/09537104.2021.1921721}},
  year         = {{2021}},
}