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Plasma variation and reproducibility of oxidized LDL-cholesterol and low-grade inflammation biomarkers among participants of the Malmö Diet and Cancer cohort

Alves Dias, Joana LU ; Hellstrand, Sophie LU ; Ericson, Ulrika LU ; Gullberg, B. LU ; Nilsson, Jan LU ; Alm, Ragnar LU ; Persson, Margaretha LU ; Engström, Gunnar LU ; Nordin Fredrikson, Gunilla LU and Hedblad, B. LU , et al. (2016) In Biomarkers 21(6). p.562-571
Abstract

Context: Large epidemiological studies often collect non-fasting samples, although the reliability of biomarkers may be uncertain. Objective: To explore the reliability and reproducibility of a single measurement of selected biomarkers in a sub-sample of the Malmö Diet and Cancer cohort. Methods: We estimated single- and average-measures intraclass correlation coefficients (ICC) for oxidized (ox)-LDL, interleukin (IL)-1β, IL-6, IL-8 and TNF-α. Results: Single-measures ICC in non-fasting samples of ox-LDL, IL-1β, IL-6, IL-8 and TNF-α were the following: 0.85, 0.71, 0.61, 0.78 and 0.66 for men, and 0.67, 0.81, 0.87, 0.69 and 0.81 for women. Biomarkers at non-fasting and fasting samples were highly correlated (all r > 0.80).... (More)

Context: Large epidemiological studies often collect non-fasting samples, although the reliability of biomarkers may be uncertain. Objective: To explore the reliability and reproducibility of a single measurement of selected biomarkers in a sub-sample of the Malmö Diet and Cancer cohort. Methods: We estimated single- and average-measures intraclass correlation coefficients (ICC) for oxidized (ox)-LDL, interleukin (IL)-1β, IL-6, IL-8 and TNF-α. Results: Single-measures ICC in non-fasting samples of ox-LDL, IL-1β, IL-6, IL-8 and TNF-α were the following: 0.85, 0.71, 0.61, 0.78 and 0.66 for men, and 0.67, 0.81, 0.87, 0.69 and 0.81 for women. Biomarkers at non-fasting and fasting samples were highly correlated (all r > 0.80). Conclusions: The observed ICC suggest that most of the examined biomarkers (non-fasting blood) would allow meaningful analysis in epidemiological studies.

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Please use this url to cite or link to this publication:
@article{c0551e3a-4881-47fe-85ee-1af156c9983c,
  abstract     = {<p>Context: Large epidemiological studies often collect non-fasting samples, although the reliability of biomarkers may be uncertain. Objective: To explore the reliability and reproducibility of a single measurement of selected biomarkers in a sub-sample of the Malmö Diet and Cancer cohort. Methods: We estimated single- and average-measures intraclass correlation coefficients (ICC) for oxidized (ox)-LDL, interleukin (IL)-1β, IL-6, IL-8 and TNF-α. Results: Single-measures ICC in non-fasting samples of ox-LDL, IL-1β, IL-6, IL-8 and TNF-α were the following: 0.85, 0.71, 0.61, 0.78 and 0.66 for men, and 0.67, 0.81, 0.87, 0.69 and 0.81 for women. Biomarkers at non-fasting and fasting samples were highly correlated (all r &gt; 0.80). Conclusions: The observed ICC suggest that most of the examined biomarkers (non-fasting blood) would allow meaningful analysis in epidemiological studies.</p>},
  author       = {Alves Dias, Joana and Hellstrand, Sophie and Ericson, Ulrika and Gullberg, B. and Nilsson, Jan and Alm, Ragnar and Persson, Margaretha and Engström, Gunnar and Nordin Fredrikson, Gunilla and Hedblad, B. and Wirfält, Elisabet},
  issn         = {1354-750X},
  keyword      = {Biomarkers of inflammation,cytokines,epidemiology,ICC,variation},
  language     = {eng},
  month        = {08},
  number       = {6},
  pages        = {562--571},
  publisher    = {Taylor & Francis},
  series       = {Biomarkers},
  title        = {Plasma variation and reproducibility of oxidized LDL-cholesterol and low-grade inflammation biomarkers among participants of the Malmö Diet and Cancer cohort},
  url          = {http://dx.doi.org/10.3109/1354750X.2016.1160431},
  volume       = {21},
  year         = {2016},
}