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Rescue Effect Inherited in Colony Formation Assays Affects Radiation Response

Adrian, Gabriel LU ; Ceberg, Crister LU ; Carneiro, Ana LU and Ekblad, Lars LU (2018) In Radiation Research 189(1). p.44-52
Abstract

It is well known that nonirradiated cells can exhibit radiation damage (bystander effect), and recent findings have shown that nonirradiated cells may help protect irradiated cells (rescue effect). These findings call into question the traditional view of radiation response: cells cannot be envisioned as isolated units. Here, we investigated traditional colony formation assays to determine if they also comprise cellular communication affecting the radiation response, using colony formation assays with varying numbers of cells, modulated beam irradiation and media transfer. Our findings showed that surviving fraction gradually increased with increasing number of irradiated cells. Specifically, for DU-145 human prostate cancer cells,... (More)

It is well known that nonirradiated cells can exhibit radiation damage (bystander effect), and recent findings have shown that nonirradiated cells may help protect irradiated cells (rescue effect). These findings call into question the traditional view of radiation response: cells cannot be envisioned as isolated units. Here, we investigated traditional colony formation assays to determine if they also comprise cellular communication affecting the radiation response, using colony formation assays with varying numbers of cells, modulated beam irradiation and media transfer. Our findings showed that surviving fraction gradually increased with increasing number of irradiated cells. Specifically, for DU-145 human prostate cancer cells, surviving fraction increased 1.9-to-4.1-fold after 5-12 Gy irradiation; and for MM576 human melanoma cells, surviving fraction increased 1.9-fold after 5 Gy irradiation. Furthermore, increased surviving fraction was evident after modulated beam irradiation, where irradiated cells could communicate with nonirradiated cells. Media from dense cell culture also increased surviving fraction. The results suggest that traditional colony formation assays comprise unavoidable cellular communication affecting radiation outcome and the shape of the survival curve. We also propose that the increased in-field surviving fraction after modulated beam irradiation is due to the same effect.

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publication status
published
subject
keywords
Journal Article
in
Radiation Research
volume
189
issue
1
pages
9 pages
publisher
Radiation Research Society
external identifiers
  • scopus:85042382522
ISSN
0033-7587
DOI
language
English
LU publication?
yes
id
c27f7d1c-8629-464f-8d28-f83560cf9304
date added to LUP
2018-01-12 14:12:44
date last changed
2018-05-29 11:30:06
@article{c27f7d1c-8629-464f-8d28-f83560cf9304,
  abstract     = {<p>It is well known that nonirradiated cells can exhibit radiation damage (bystander effect), and recent findings have shown that nonirradiated cells may help protect irradiated cells (rescue effect). These findings call into question the traditional view of radiation response: cells cannot be envisioned as isolated units. Here, we investigated traditional colony formation assays to determine if they also comprise cellular communication affecting the radiation response, using colony formation assays with varying numbers of cells, modulated beam irradiation and media transfer. Our findings showed that surviving fraction gradually increased with increasing number of irradiated cells. Specifically, for DU-145 human prostate cancer cells, surviving fraction increased 1.9-to-4.1-fold after 5-12 Gy irradiation; and for MM576 human melanoma cells, surviving fraction increased 1.9-fold after 5 Gy irradiation. Furthermore, increased surviving fraction was evident after modulated beam irradiation, where irradiated cells could communicate with nonirradiated cells. Media from dense cell culture also increased surviving fraction. The results suggest that traditional colony formation assays comprise unavoidable cellular communication affecting radiation outcome and the shape of the survival curve. We also propose that the increased in-field surviving fraction after modulated beam irradiation is due to the same effect.</p>},
  author       = {Adrian, Gabriel and Ceberg, Crister and Carneiro, Ana and Ekblad, Lars},
  issn         = {0033-7587},
  keyword      = {Journal Article},
  language     = {eng},
  number       = {1},
  pages        = {44--52},
  publisher    = {Radiation Research Society},
  series       = {Radiation Research},
  title        = {Rescue Effect Inherited in Colony Formation Assays Affects Radiation Response},
  url          = {http://dx.doi.org/},
  volume       = {189},
  year         = {2018},
}