Subtilisin inhibitors from legume seeds : A purification procedure
(1985) In Journal of the Science of Food and Agriculture 36(12). p.1285-1290- Abstract
Subtilisin inhibitors were prepared from ethanol‐acetone precipitated seed extracts of chick peas, jack beans and broad beans, heated to destroy endogenous proteases. Trypsin inhibitors were complexed with trypsin and separated from subtilisin inhibitors by molecular sieve chromatography. The eluates were again heated to avoid tryptic degradation. The procedure was easy to handle and gave satisfactory yields. In a final step two subtilisin isoinhibitors from chick peas were purified to electrophoretic homogeneity. They constitute 0.01% of the defatted dry weight.
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- author
- Tovar, Juscelino LU ; Lorenzo, Pilar LU and Seidl, Dinah S.
- publishing date
- 1985-01-01
- type
- Contribution to journal
- publication status
- published
- keywords
- broad beans, chick peas, jack beans, Proteinase inhibitors, purification procedure, subtilisin inhibitors
- in
- Journal of the Science of Food and Agriculture
- volume
- 36
- issue
- 12
- pages
- 6 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:84985387035
- ISSN
- 0022-5142
- DOI
- 10.1002/jsfa.2740361213
- language
- English
- LU publication?
- no
- id
- c7e3bce4-766e-423d-8148-ac1c9b839a87
- date added to LUP
- 2018-10-05 16:46:45
- date last changed
- 2024-01-15 02:55:21
@article{c7e3bce4-766e-423d-8148-ac1c9b839a87, abstract = {{<p>Subtilisin inhibitors were prepared from ethanol‐acetone precipitated seed extracts of chick peas, jack beans and broad beans, heated to destroy endogenous proteases. Trypsin inhibitors were complexed with trypsin and separated from subtilisin inhibitors by molecular sieve chromatography. The eluates were again heated to avoid tryptic degradation. The procedure was easy to handle and gave satisfactory yields. In a final step two subtilisin isoinhibitors from chick peas were purified to electrophoretic homogeneity. They constitute 0.01% of the defatted dry weight.</p>}}, author = {{Tovar, Juscelino and Lorenzo, Pilar and Seidl, Dinah S.}}, issn = {{0022-5142}}, keywords = {{broad beans; chick peas; jack beans; Proteinase inhibitors; purification procedure; subtilisin inhibitors}}, language = {{eng}}, month = {{01}}, number = {{12}}, pages = {{1285--1290}}, publisher = {{Wiley-Blackwell}}, series = {{Journal of the Science of Food and Agriculture}}, title = {{Subtilisin inhibitors from legume seeds : A purification procedure}}, url = {{http://dx.doi.org/10.1002/jsfa.2740361213}}, doi = {{10.1002/jsfa.2740361213}}, volume = {{36}}, year = {{1985}}, }