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Bilberry Supplementation after Myocardial Infarction Decreases Microvesicles in Blood and Affects Endothelial Vesiculation

Bryl-Górecka, Paulina LU ; Sathanoori, Ramasri LU ; Arevström, Lilith ; Landberg, Rikard ; Bergh, Cecilia ; Evander, Mikael LU ; Olde, Björn LU ; Laurell, Thomas LU ; Fröbert, Ole and Erlinge, David LU orcid (2020) In Molecular Nutrition & Food Research 64(20).
Abstract

Scope: Diet rich in bilberries is considered cardioprotective, but the mechanisms of action are poorly understood. Cardiovascular disease is characterized by increased proatherogenic status and high levels of circulating microvesicles (MVs). In an open-label study patients with myocardial infarction receive an 8 week dietary supplementation with bilberry extract (BE). The effect of BE on patient MV levels and its influence on endothelial vesiculation in vitro is investigated. Methods and results: MVs are captured with acoustic trapping and platelet-derived MVs (PMVs), as well as endothelial-derived MVs (EMVs) are quantified with flow cytometry. The in vitro effect of BE on endothelial extracellular vesicle (EV) release is examined using... (More)

Scope: Diet rich in bilberries is considered cardioprotective, but the mechanisms of action are poorly understood. Cardiovascular disease is characterized by increased proatherogenic status and high levels of circulating microvesicles (MVs). In an open-label study patients with myocardial infarction receive an 8 week dietary supplementation with bilberry extract (BE). The effect of BE on patient MV levels and its influence on endothelial vesiculation in vitro is investigated. Methods and results: MVs are captured with acoustic trapping and platelet-derived MVs (PMVs), as well as endothelial-derived MVs (EMVs) are quantified with flow cytometry. The in vitro effect of BE on endothelial extracellular vesicle (EV) release is examined using endothelial cells and calcein staining. The mechanisms of BE influence on vesiculation pathways are studied by Western blot and qRT-PCR. Supplementation with BE decreased both PMVs and EMVs. Furthermore, BE reduced endothelial EV release, Akt phosphorylation, and vesiculation-related gene transcription. It also protects the cells from P2X7-induced EV release and increase in vesiculation-related gene expression. Conclusion: BE supplementation improves the MV profile in patient blood and reduces endothelial vesiculation through several molecular mechanisms related to the P2X7 receptor. The findings provide new insight into the cardioprotective effects of bilberries.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bilberries, cardiovascular diseases, microvesicles, P2X (P2X purinoreceptor 7)
in
Molecular Nutrition & Food Research
volume
64
issue
20
article number
2000108
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85090439473
  • pmid:32846041
ISSN
1613-4125
DOI
10.1002/mnfr.202000108
language
English
LU publication?
yes
id
ca71770a-aaeb-453d-868e-a01e87fc0d65
date added to LUP
2020-10-02 12:34:02
date last changed
2024-06-13 23:51:43
@article{ca71770a-aaeb-453d-868e-a01e87fc0d65,
  abstract     = {{<p>Scope: Diet rich in bilberries is considered cardioprotective, but the mechanisms of action are poorly understood. Cardiovascular disease is characterized by increased proatherogenic status and high levels of circulating microvesicles (MVs). In an open-label study patients with myocardial infarction receive an 8 week dietary supplementation with bilberry extract (BE). The effect of BE on patient MV levels and its influence on endothelial vesiculation in vitro is investigated. Methods and results: MVs are captured with acoustic trapping and platelet-derived MVs (PMVs), as well as endothelial-derived MVs (EMVs) are quantified with flow cytometry. The in vitro effect of BE on endothelial extracellular vesicle (EV) release is examined using endothelial cells and calcein staining. The mechanisms of BE influence on vesiculation pathways are studied by Western blot and qRT-PCR. Supplementation with BE decreased both PMVs and EMVs. Furthermore, BE reduced endothelial EV release, Akt phosphorylation, and vesiculation-related gene transcription. It also protects the cells from P2X<sub>7</sub>-induced EV release and increase in vesiculation-related gene expression. Conclusion: BE supplementation improves the MV profile in patient blood and reduces endothelial vesiculation through several molecular mechanisms related to the P2X<sub>7</sub> receptor. The findings provide new insight into the cardioprotective effects of bilberries.</p>}},
  author       = {{Bryl-Górecka, Paulina and Sathanoori, Ramasri and Arevström, Lilith and Landberg, Rikard and Bergh, Cecilia and Evander, Mikael and Olde, Björn and Laurell, Thomas and Fröbert, Ole and Erlinge, David}},
  issn         = {{1613-4125}},
  keywords     = {{bilberries; cardiovascular diseases; microvesicles; P2X (P2X purinoreceptor 7)}},
  language     = {{eng}},
  number       = {{20}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Molecular Nutrition & Food Research}},
  title        = {{Bilberry Supplementation after Myocardial Infarction Decreases Microvesicles in Blood and Affects Endothelial Vesiculation}},
  url          = {{http://dx.doi.org/10.1002/mnfr.202000108}},
  doi          = {{10.1002/mnfr.202000108}},
  volume       = {{64}},
  year         = {{2020}},
}