Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Toxicity of particles derived from combustion of Ethiopian traditional biomass fuels in human bronchial and macrophage-like cells

McCarrick, Sarah ; Delaval, Mathilde N. ; Dauter, Ulrike M. ; Krais, Annette M. LU orcid ; Snigireva, Anastasiia ; Abera, Asmamaw ; Broberg, Karin LU orcid ; Eriksson, Axel C. LU orcid ; Isaxon, Christina LU and Gliga, Anda R. LU (2024) In Archives of Toxicology
Abstract

The combustion of traditional fuels in low-income countries, including those in sub-Saharan Africa, leads to extensive indoor particle exposure. Yet, the related health consequences in this context are understudied. This study aimed to evaluate the in vitro toxicity of combustion-derived particles relevant for Sub-Saharan household environments. Particles (< 2.5 µm) were collected using a high-volume sampler during combustion of traditional Ethiopian biomass fuels: cow dung, eucalyptus wood and eucalyptus charcoal. Diesel exhaust particles (DEP, NIST 2975) served as reference particles. The highest levels of particle-bound polycyclic aromatic hydrocarbons (PAHs) were found in wood (3219 ng/mg), followed by dung (618 ng/mg), charcoal... (More)

The combustion of traditional fuels in low-income countries, including those in sub-Saharan Africa, leads to extensive indoor particle exposure. Yet, the related health consequences in this context are understudied. This study aimed to evaluate the in vitro toxicity of combustion-derived particles relevant for Sub-Saharan household environments. Particles (< 2.5 µm) were collected using a high-volume sampler during combustion of traditional Ethiopian biomass fuels: cow dung, eucalyptus wood and eucalyptus charcoal. Diesel exhaust particles (DEP, NIST 2975) served as reference particles. The highest levels of particle-bound polycyclic aromatic hydrocarbons (PAHs) were found in wood (3219 ng/mg), followed by dung (618 ng/mg), charcoal (136 ng/mg) and DEP (118 ng/mg) (GC–MS). BEAS-2B bronchial epithelial cells and THP-1 derived macrophages were exposed to particle suspensions (1–150 µg/mL) for 24 h. All particles induced concentration-dependent genotoxicity (comet assay) but no pro-inflammatory cytokine release in epithelial cells, whereas dung and wood particles also induced concentration-dependent cytotoxicity (Alamar Blue). Only wood particles induced concentration-dependent cytotoxicity and genotoxicity in macrophage-like cells, while dung particles were unique at increasing secretion of pro-inflammatory cytokines (IL-6, IL-8, TNF-α). In summary, particles derived from combustion of less energy dense fuels like dung and wood had a higher PAH content and were more cytotoxic in epithelial cells. In addition, the least energy dense and cheapest fuel, dung, also induced pro-inflammatory effects in macrophage-like cells. These findings highlight the influence of fuel type on the toxic profile of the emitted particles and warrant further research to understand and mitigate health effects of indoor air pollution.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Combustion-derived particles, Cytokines, Genotoxicity, Polycyclic aromatic hydrocarbons (PAH), Solid biomass fuel, Toxicity
in
Archives of Toxicology
publisher
Springer
external identifiers
  • pmid:38427118
  • scopus:85186437008
ISSN
0340-5761
DOI
10.1007/s00204-024-03692-8
language
English
LU publication?
yes
id
cd4f657f-d5fa-4ae4-a4a8-753d813c279c
date added to LUP
2024-03-20 15:44:51
date last changed
2024-04-17 15:23:38
@article{cd4f657f-d5fa-4ae4-a4a8-753d813c279c,
  abstract     = {{<p>The combustion of traditional fuels in low-income countries, including those in sub-Saharan Africa, leads to extensive indoor particle exposure. Yet, the related health consequences in this context are understudied. This study aimed to evaluate the in vitro toxicity of combustion-derived particles relevant for Sub-Saharan household environments. Particles (&lt; 2.5 µm) were collected using a high-volume sampler during combustion of traditional Ethiopian biomass fuels: cow dung, eucalyptus wood and eucalyptus charcoal. Diesel exhaust particles (DEP, NIST 2975) served as reference particles. The highest levels of particle-bound polycyclic aromatic hydrocarbons (PAHs) were found in wood (3219 ng/mg), followed by dung (618 ng/mg), charcoal (136 ng/mg) and DEP (118 ng/mg) (GC–MS). BEAS-2B bronchial epithelial cells and THP-1 derived macrophages were exposed to particle suspensions (1–150 µg/mL) for 24 h. All particles induced concentration-dependent genotoxicity (comet assay) but no pro-inflammatory cytokine release in epithelial cells, whereas dung and wood particles also induced concentration-dependent cytotoxicity (Alamar Blue). Only wood particles induced concentration-dependent cytotoxicity and genotoxicity in macrophage-like cells, while dung particles were unique at increasing secretion of pro-inflammatory cytokines (IL-6, IL-8, TNF-α). In summary, particles derived from combustion of less energy dense fuels like dung and wood had a higher PAH content and were more cytotoxic in epithelial cells. In addition, the least energy dense and cheapest fuel, dung, also induced pro-inflammatory effects in macrophage-like cells. These findings highlight the influence of fuel type on the toxic profile of the emitted particles and warrant further research to understand and mitigate health effects of indoor air pollution.</p>}},
  author       = {{McCarrick, Sarah and Delaval, Mathilde N. and Dauter, Ulrike M. and Krais, Annette M. and Snigireva, Anastasiia and Abera, Asmamaw and Broberg, Karin and Eriksson, Axel C. and Isaxon, Christina and Gliga, Anda R.}},
  issn         = {{0340-5761}},
  keywords     = {{Combustion-derived particles; Cytokines; Genotoxicity; Polycyclic aromatic hydrocarbons (PAH); Solid biomass fuel; Toxicity}},
  language     = {{eng}},
  publisher    = {{Springer}},
  series       = {{Archives of Toxicology}},
  title        = {{Toxicity of particles derived from combustion of Ethiopian traditional biomass fuels in human bronchial and macrophage-like cells}},
  url          = {{http://dx.doi.org/10.1007/s00204-024-03692-8}},
  doi          = {{10.1007/s00204-024-03692-8}},
  year         = {{2024}},
}