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Multiomic profiling of ER-positive HER2-negative breast cancer reveals markers associated with metastatic spread

Mosquim Junior, Sergio LU orcid ; Zamore, Måns LU orcid ; Vallon-Christersson, Johan LU orcid ; Rydén, Lisa LU orcid and Levander, Fredrik LU orcid (2026) In Breast Cancer Research 28(1).
Abstract

Background: Metastatic disease is the main cause of breast cancer (BC)-related deaths, but prediction of metastases remains challenging especially in the large and diverse group with estrogen receptor (ER)-positive, human epidermal growth factor receptor 2 (HER2)-negative tumors. Molecular tumor features beyond currently used markers could provide important information for stratifying metastatic risk. To allow for the discovery of new subtypes and molecular tumor features associated with metastatic spread, i.e., both lymph node and distant metastases, we here leverage advances in proteomic profiling of tumors. Methods: We developed a protocol for proteome and phosphoproteome analysis using label-free data independent acquisition (DIA)... (More)

Background: Metastatic disease is the main cause of breast cancer (BC)-related deaths, but prediction of metastases remains challenging especially in the large and diverse group with estrogen receptor (ER)-positive, human epidermal growth factor receptor 2 (HER2)-negative tumors. Molecular tumor features beyond currently used markers could provide important information for stratifying metastatic risk. To allow for the discovery of new subtypes and molecular tumor features associated with metastatic spread, i.e., both lymph node and distant metastases, we here leverage advances in proteomic profiling of tumors. Methods: We developed a protocol for proteome and phosphoproteome analysis using label-free data independent acquisition (DIA) liquid chromatography tandem mass spectrometry (LC–MS/MS) and integrated the generated data with parallel transcriptome data for the profiling of 182 ER-positive, HER2-negative primary BC tumors from the SCAN-B cohort. Results: A total of 13,571 protein groups, 7107 phosphopeptides and 13,085 expressed genes were quantified in at least 70% of the samples. The data showed clear differences between invasive lobular carcinoma and no special type cancers, including the hallmark loss of E-cadherin expression and differences in catenin levels. We identified potential new subtypes with differential immune infiltration patterns and survival through unsupervised consensus clustering. Additionally, by adopting an integrative, multiomic data analysis workflow, we identified several potential protein markers of both lymph node and distant metastases. For lymph node metastasis, the level of phosphorylated ES8L2 serine at position 570 (multivariable p value = 0.05, HR = 0.61, 95% CI 0.38–0.99) was associated with improved recurrence-free survival, and showed decreased abundance in lymph node positive cases. For distant metastases, on the other hand, proteins belonging to the heat shock protein 90 family were associated with worse distant recurrence-free survival (multivariable p value = 0.0058, HR = 2.10, 95% CI 1.24–3.55), with significantly higher abundance levels in patients with a distant recurrence event. These correlations with survival could also be validated in multiple external cohorts. Conclusions: In summary, we present the most comprehensive matched multiomic dataset from ER-positive/HER2-negative BC tumors, not only serving as an invaluable resource for further advancing precision medicine but also allowing the discovery of potential biomarkers and providing unique insights into metastatic processes. Trial registration: Sweden Cancerome Analysis Network—Breast: Genomic Profiling of Breast Cancer (SCAN-B), beginning 2010-08, NCT02306096.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Automation, Biomarker discovery, Breast cancer, Deconvolution, Immune infiltration, Mass spectrometry, Metastasis, Multi-omics, Phosphoproteomics, Proteomics
in
Breast Cancer Research
volume
28
issue
1
article number
12
publisher
BioMed Central (BMC)
external identifiers
  • scopus:105027589914
  • pmid:41526910
ISSN
1465-5411
DOI
10.1186/s13058-025-02173-9
language
English
LU publication?
yes
additional info
Publisher Copyright: © The Author(s) 2026.
id
cee5d1c5-54e9-4730-928b-6f027f997ab3
date added to LUP
2026-03-09 13:55:09
date last changed
2026-06-02 02:28:01
@article{cee5d1c5-54e9-4730-928b-6f027f997ab3,
  abstract     = {{<p>Background: Metastatic disease is the main cause of breast cancer (BC)-related deaths, but prediction of metastases remains challenging especially in the large and diverse group with estrogen receptor (ER)-positive, human epidermal growth factor receptor 2 (HER2)-negative tumors. Molecular tumor features beyond currently used markers could provide important information for stratifying metastatic risk. To allow for the discovery of new subtypes and molecular tumor features associated with metastatic spread, i.e., both lymph node and distant metastases, we here leverage advances in proteomic profiling of tumors. Methods: We developed a protocol for proteome and phosphoproteome analysis using label-free data independent acquisition (DIA) liquid chromatography tandem mass spectrometry (LC–MS/MS) and integrated the generated data with parallel transcriptome data for the profiling of 182 ER-positive, HER2-negative primary BC tumors from the SCAN-B cohort. Results: A total of 13,571 protein groups, 7107 phosphopeptides and 13,085 expressed genes were quantified in at least 70% of the samples. The data showed clear differences between invasive lobular carcinoma and no special type cancers, including the hallmark loss of E-cadherin expression and differences in catenin levels. We identified potential new subtypes with differential immune infiltration patterns and survival through unsupervised consensus clustering. Additionally, by adopting an integrative, multiomic data analysis workflow, we identified several potential protein markers of both lymph node and distant metastases. For lymph node metastasis, the level of phosphorylated ES8L2 serine at position 570 (multivariable p value = 0.05, HR = 0.61, 95% CI 0.38–0.99) was associated with improved recurrence-free survival, and showed decreased abundance in lymph node positive cases. For distant metastases, on the other hand, proteins belonging to the heat shock protein 90 family were associated with worse distant recurrence-free survival (multivariable p value = 0.0058, HR = 2.10, 95% CI 1.24–3.55), with significantly higher abundance levels in patients with a distant recurrence event. These correlations with survival could also be validated in multiple external cohorts. Conclusions: In summary, we present the most comprehensive matched multiomic dataset from ER-positive/HER2-negative BC tumors, not only serving as an invaluable resource for further advancing precision medicine but also allowing the discovery of potential biomarkers and providing unique insights into metastatic processes. Trial registration: Sweden Cancerome Analysis Network—Breast: Genomic Profiling of Breast Cancer (SCAN-B), beginning 2010-08, NCT02306096.</p>}},
  author       = {{Mosquim Junior, Sergio and Zamore, Måns and Vallon-Christersson, Johan and Rydén, Lisa and Levander, Fredrik}},
  issn         = {{1465-5411}},
  keywords     = {{Automation; Biomarker discovery; Breast cancer; Deconvolution; Immune infiltration; Mass spectrometry; Metastasis; Multi-omics; Phosphoproteomics; Proteomics}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{BioMed Central (BMC)}},
  series       = {{Breast Cancer Research}},
  title        = {{Multiomic profiling of ER-positive HER2-negative breast cancer reveals markers associated with metastatic spread}},
  url          = {{http://dx.doi.org/10.1186/s13058-025-02173-9}},
  doi          = {{10.1186/s13058-025-02173-9}},
  volume       = {{28}},
  year         = {{2026}},
}