Haemophilus influenzae P4 interacts with extracellular matrix proteins promoting adhesion and serum resistance.
(2016) In Journal of Infectious Diseases 213(2). p.314-323- Abstract
- Interaction with the extracellular matrix (ECM) is one of the successful colonization strategies employed by non-typeable Haemophilus influenzae (NTHi). Here we identified Haemophilus lipoprotein 4 (P4) as a receptor for ECM proteins. Purified recombinant P4 displayed a high binding affinity for laminin (Kd=9.26 nM) and fibronectin (Kd=10.19 nM), but slightly less to vitronectin (Kd=16.51 nM). A P4-deficient NTHi mutant showed a significantly decreased binding to these ECM components. Vitronectin acquisition conferred serum resistance to both P4-expressing NTHi and Escherichia coli transformants. P4-mediated bacterial adherence to pharynx, type II alveolar and bronchial epithelial cells was mainly attributed to fibronectin. Importantly, a... (More)
- Interaction with the extracellular matrix (ECM) is one of the successful colonization strategies employed by non-typeable Haemophilus influenzae (NTHi). Here we identified Haemophilus lipoprotein 4 (P4) as a receptor for ECM proteins. Purified recombinant P4 displayed a high binding affinity for laminin (Kd=9.26 nM) and fibronectin (Kd=10.19 nM), but slightly less to vitronectin (Kd=16.51 nM). A P4-deficient NTHi mutant showed a significantly decreased binding to these ECM components. Vitronectin acquisition conferred serum resistance to both P4-expressing NTHi and Escherichia coli transformants. P4-mediated bacterial adherence to pharynx, type II alveolar and bronchial epithelial cells was mainly attributed to fibronectin. Importantly, a significantly reduced bacterial infection was observed in the middle ear of the Junbo mouse model when NTHi was devoid of P4. In conclusion, our data provide new insight into the role of P4 as an important factor for Haemophilus colonization and subsequent respiratory tract infection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7750162
- author
- Su, Shanice YC LU ; Mukherjee, Oindrilla LU ; Singh, Birendra LU ; Hallgren, Oskar LU ; Westergren-Thorsson, Gunilla LU ; Hood, Derek and Riesbeck, Kristian LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 213
- issue
- 2
- pages
- 314 - 323
- publisher
- Oxford University Press
- external identifiers
-
- pmid:26153407
- scopus:84959924526
- wos:000371237900019
- pmid:26153407
- ISSN
- 1537-6613
- DOI
- 10.1093/infdis/jiv374
- language
- English
- LU publication?
- yes
- id
- d032a256-62ad-4355-bca0-c4b978846304 (old id 7750162)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26153407?dopt=Abstract
- date added to LUP
- 2016-04-04 09:31:39
- date last changed
- 2024-06-08 13:00:11
@article{d032a256-62ad-4355-bca0-c4b978846304, abstract = {{Interaction with the extracellular matrix (ECM) is one of the successful colonization strategies employed by non-typeable Haemophilus influenzae (NTHi). Here we identified Haemophilus lipoprotein 4 (P4) as a receptor for ECM proteins. Purified recombinant P4 displayed a high binding affinity for laminin (Kd=9.26 nM) and fibronectin (Kd=10.19 nM), but slightly less to vitronectin (Kd=16.51 nM). A P4-deficient NTHi mutant showed a significantly decreased binding to these ECM components. Vitronectin acquisition conferred serum resistance to both P4-expressing NTHi and Escherichia coli transformants. P4-mediated bacterial adherence to pharynx, type II alveolar and bronchial epithelial cells was mainly attributed to fibronectin. Importantly, a significantly reduced bacterial infection was observed in the middle ear of the Junbo mouse model when NTHi was devoid of P4. In conclusion, our data provide new insight into the role of P4 as an important factor for Haemophilus colonization and subsequent respiratory tract infection.}}, author = {{Su, Shanice YC and Mukherjee, Oindrilla and Singh, Birendra and Hallgren, Oskar and Westergren-Thorsson, Gunilla and Hood, Derek and Riesbeck, Kristian}}, issn = {{1537-6613}}, language = {{eng}}, number = {{2}}, pages = {{314--323}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{Haemophilus influenzae P4 interacts with extracellular matrix proteins promoting adhesion and serum resistance.}}, url = {{http://dx.doi.org/10.1093/infdis/jiv374}}, doi = {{10.1093/infdis/jiv374}}, volume = {{213}}, year = {{2016}}, }