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The importance of a β-glucan receptor in the nonopsonic entry of nontypeable Haemophilus influenzae into human monocytic and epithelial cells

Ahrén, Irini Lazou LU ; Williams, David L. ; Rice, Peter J. ; Forsgren, Arne LU and Riesbeck, Kristian LU (2001) In Journal of Infectious Diseases 184(2). p.150-158
Abstract

Previous reports showed that nontypeable Haemophilus influenzae (NTHi) reside in macrophage-like cells in human adenoid tissue. This study investigated the ability of nonopsonized NTHi and encapsulated H. influenzae type b (Hib) to enter human monocytic and epithelial cells. The number of intracellular bacteria was determined by a viability assay and flow cytometry. To characterize the mechanisms responsible for the internalization of NTHi, different inhibitors of surface molecules, receptor turnover, and the cytoskeleton were used. Hib were found in monocytic cells at very low numbers (<100 bacteria/2 × 105 cells). In contrast, a great variation in intracellular numbers was detected between the different NTHi isolates... (More)

Previous reports showed that nontypeable Haemophilus influenzae (NTHi) reside in macrophage-like cells in human adenoid tissue. This study investigated the ability of nonopsonized NTHi and encapsulated H. influenzae type b (Hib) to enter human monocytic and epithelial cells. The number of intracellular bacteria was determined by a viability assay and flow cytometry. To characterize the mechanisms responsible for the internalization of NTHi, different inhibitors of surface molecules, receptor turnover, and the cytoskeleton were used. Hib were found in monocytic cells at very low numbers (<100 bacteria/2 × 105 cells). In contrast, a great variation in intracellular numbers was detected between the different NTHi isolates (range, 0.0007%-0.28% of the inoculum for monocytes and 0.053%-3.5% for epithelial cells). NTHi entered human monocytic and epithelial cells via a receptor-mediated endocytosis involving mainly a β-glucan receptor that could be blocked by laminarin.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
184
issue
2
pages
150 - 158
publisher
Oxford University Press
external identifiers
  • pmid:11424011
  • scopus:0035879824
ISSN
0022-1899
DOI
10.1086/322016
language
English
LU publication?
yes
id
d064482a-1045-44a9-893b-2feb1bd16013
date added to LUP
2019-06-07 15:09:57
date last changed
2020-07-16 03:27:16
@article{d064482a-1045-44a9-893b-2feb1bd16013,
  abstract     = {<p>Previous reports showed that nontypeable Haemophilus influenzae (NTHi) reside in macrophage-like cells in human adenoid tissue. This study investigated the ability of nonopsonized NTHi and encapsulated H. influenzae type b (Hib) to enter human monocytic and epithelial cells. The number of intracellular bacteria was determined by a viability assay and flow cytometry. To characterize the mechanisms responsible for the internalization of NTHi, different inhibitors of surface molecules, receptor turnover, and the cytoskeleton were used. Hib were found in monocytic cells at very low numbers (&lt;100 bacteria/2 × 10<sup>5</sup> cells). In contrast, a great variation in intracellular numbers was detected between the different NTHi isolates (range, 0.0007%-0.28% of the inoculum for monocytes and 0.053%-3.5% for epithelial cells). NTHi entered human monocytic and epithelial cells via a receptor-mediated endocytosis involving mainly a β-glucan receptor that could be blocked by laminarin.</p>},
  author       = {Ahrén, Irini Lazou and Williams, David L. and Rice, Peter J. and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {0022-1899},
  language     = {eng},
  month        = {07},
  number       = {2},
  pages        = {150--158},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {The importance of a β-glucan receptor in the nonopsonic entry of nontypeable Haemophilus influenzae into human monocytic and epithelial cells},
  url          = {http://dx.doi.org/10.1086/322016},
  doi          = {10.1086/322016},
  volume       = {184},
  year         = {2001},
}