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Expression profiling of cell cycle genes in human pancreatic islets with and without type 2 diabetes.

Taneera, Jalal LU ; Fadista, Joao LU ; Ahlqvist, Emma LU ; Zhang, Mengze; Wierup, Nils LU ; Renström, Erik LU and Groop, Leif LU (2013) In Molecular and Cellular Endocrinology 375(1-2). p.35-42
Abstract
Microarray gene expression data were used to analyze the expression pattern of cyclin, cyclin-dependent kinase (CDKs) and cyclin-dependent kinase inhibitor (CDKIs) genes from human pancreatic islets with and without type 2 diabetes (T2D). Of the cyclin genes, CCNI was the most expressed. Data obtained from microarray and qRT-PCR showed higher expression of CCND1 in diabetic islets. Among the CDKs, CDK4, CDK8 and CDK9 were highly expressed, while CDK1 was expressed at low level. High expression of CDK18 was observed in diabetic islets. Of the CDKIs, CDKN1A expression was higher in diabetic islets in both microarray and qRT-PCR. Expression of CDKN1A, CDKN2A, CCNI2, CDK3 and CDK16 was correlated with age. Finally, eight SNPs in these genes... (More)
Microarray gene expression data were used to analyze the expression pattern of cyclin, cyclin-dependent kinase (CDKs) and cyclin-dependent kinase inhibitor (CDKIs) genes from human pancreatic islets with and without type 2 diabetes (T2D). Of the cyclin genes, CCNI was the most expressed. Data obtained from microarray and qRT-PCR showed higher expression of CCND1 in diabetic islets. Among the CDKs, CDK4, CDK8 and CDK9 were highly expressed, while CDK1 was expressed at low level. High expression of CDK18 was observed in diabetic islets. Of the CDKIs, CDKN1A expression was higher in diabetic islets in both microarray and qRT-PCR. Expression of CDKN1A, CDKN2A, CCNI2, CDK3 and CDK16 was correlated with age. Finally, eight SNPs in these genes were associated with T2D in the DIAGRAM database. Our data provide a comprehensive expression pattern of cell cycle genes in human islets. More human studies are required to confirm and reproduce animal studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Endocrinology
volume
375
issue
1-2
pages
35 - 42
publisher
Elsevier
external identifiers
  • wos:000322054600005
  • pmid:23707792
  • scopus:84878699515
ISSN
1872-8057
DOI
10.1016/j.mce.2013.05.003
language
English
LU publication?
yes
id
da6bde68-7d96-4f01-8baf-9b3e3b0f532d (old id 3804127)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23707792?dopt=Abstract
date added to LUP
2013-06-05 14:10:47
date last changed
2019-08-14 01:31:05
@article{da6bde68-7d96-4f01-8baf-9b3e3b0f532d,
  abstract     = {Microarray gene expression data were used to analyze the expression pattern of cyclin, cyclin-dependent kinase (CDKs) and cyclin-dependent kinase inhibitor (CDKIs) genes from human pancreatic islets with and without type 2 diabetes (T2D). Of the cyclin genes, CCNI was the most expressed. Data obtained from microarray and qRT-PCR showed higher expression of CCND1 in diabetic islets. Among the CDKs, CDK4, CDK8 and CDK9 were highly expressed, while CDK1 was expressed at low level. High expression of CDK18 was observed in diabetic islets. Of the CDKIs, CDKN1A expression was higher in diabetic islets in both microarray and qRT-PCR. Expression of CDKN1A, CDKN2A, CCNI2, CDK3 and CDK16 was correlated with age. Finally, eight SNPs in these genes were associated with T2D in the DIAGRAM database. Our data provide a comprehensive expression pattern of cell cycle genes in human islets. More human studies are required to confirm and reproduce animal studies.},
  author       = {Taneera, Jalal and Fadista, Joao and Ahlqvist, Emma and Zhang, Mengze and Wierup, Nils and Renström, Erik and Groop, Leif},
  issn         = {1872-8057},
  language     = {eng},
  number       = {1-2},
  pages        = {35--42},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Endocrinology},
  title        = {Expression profiling of cell cycle genes in human pancreatic islets with and without type 2 diabetes.},
  url          = {http://dx.doi.org/10.1016/j.mce.2013.05.003},
  volume       = {375},
  year         = {2013},
}