Pancreatic beta-cell function evaluated by intravenous glucose and glucagon stimulation. A comparison between insulin and c-peptide to measure insulin secretion
Gottsäter, A. LU ; Landin-Olsson, M. LU ; Fernlund, P. LU ; Gullberg, B. LU ; Lernmark, Å LU
and Sundkvist, G.
LU
(1992)
In Scandinavian Journal of Clinical and Laboratory Investigation
52(7).
p.631-639
- Abstract
Insulin and C-peptide responses to 0.5 g kg-1 intravenous glucose and 1.0 mg glucagon were studied in 34 healthy subjects (age 19-78 years, mean 45). Fasting blood glucose (r=0.59; p<0.001) and glycosylated haemoglobin (r=0.61; p<0.001) increased with age, but not the initial C-peptide and insulin responses to the glucose infusion. However, the C-peptide response at 70 min (r=0.36; p<0.05), 80 min (r=0.41; p<0.05), and 90 min (r=0.46; p<0.01) after the glucose infusion correlated with age as well as both insulin (r=0.42; p<0.05) and C-peptide (r=0.45; p<0.05) responses to the glucagon injection. Reproducibility of insulin and C-peptide responses was evaluated by duplicate tests, separated 2-143 days in... (More)
Insulin and C-peptide responses to 0.5 g kg-1 intravenous glucose and 1.0 mg glucagon were studied in 34 healthy subjects (age 19-78 years, mean 45). Fasting blood glucose (r=0.59; p<0.001) and glycosylated haemoglobin (r=0.61; p<0.001) increased with age, but not the initial C-peptide and insulin responses to the glucose infusion. However, the C-peptide response at 70 min (r=0.36; p<0.05), 80 min (r=0.41; p<0.05), and 90 min (r=0.46; p<0.01) after the glucose infusion correlated with age as well as both insulin (r=0.42; p<0.05) and C-peptide (r=0.45; p<0.05) responses to the glucagon injection. Reproducibility of insulin and C-peptide responses was evaluated by duplicate tests, separated 2-143 days in time, in 10 healthy subjects (age 19-48 years, mean 32 years) showing no significant differences in median within-subject variation between the initial (1+3 min) or overall (0-90 min area under curve) insulin (24% and 17% respectively) and C-peptide (15% and 14% respectively) responses to glucose, while the within-subject variation for the fasting values and the response to glucagon was higher (p<0.05) for insulin (47% and 32% respectively) than C-peptide (13% and 14% respectively). Between-subject variation was also lower (p<0.001) for C-peptide than for insulin. Thus, C-peptide measurements in healthy subjects are more reproducible than insulin measurements in determination of beta-cell function.
(Less)
- author
- Gottsäter, A.
LU
; Landin-Olsson, M.
LU
; Fernlund, P.
LU
; Gullberg, B.
LU
; Lernmark, Å
LU
and Sundkvist, G.
LU
- organization
- publishing date
- 1992-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Glucagon stimulation, Glucose tolerance test, Reproducibility
- in
- Scandinavian Journal of Clinical and Laboratory Investigation
- volume
- 52
- issue
- 7
- pages
- 9 pages
- publisher
- Informa Healthcare
- external identifiers
-
- scopus:0026471853
- pmid:1455155
- ISSN
- 0036-5513
- DOI
- 10.3109/00365519209115506
- language
- English
- LU publication?
- yes
- id
- daddccbc-d0df-4b43-9216-12d0c2ac7b78
- date added to LUP
- 2019-12-12 23:20:54
- date last changed
- 2024-03-13 08:18:55
@article{daddccbc-d0df-4b43-9216-12d0c2ac7b78,
abstract = {{<p>Insulin and C-peptide responses to 0.5 g kg<sup>-1</sup> intravenous glucose and 1.0 mg glucagon were studied in 34 healthy subjects (age 19-78 years, mean 45). Fasting blood glucose (r=0.59; p<0.001) and glycosylated haemoglobin (r=0.61; p<0.001) increased with age, but not the initial C-peptide and insulin responses to the glucose infusion. However, the C-peptide response at 70 min (r=0.36; p<0.05), 80 min (r=0.41; p<0.05), and 90 min (r=0.46; p<0.01) after the glucose infusion correlated with age as well as both insulin (r=0.42; p<0.05) and C-peptide (r=0.45; p<0.05) responses to the glucagon injection. Reproducibility of insulin and C-peptide responses was evaluated by duplicate tests, separated 2-143 days in time, in 10 healthy subjects (age 19-48 years, mean 32 years) showing no significant differences in median within-subject variation between the initial (1+3 min) or overall (0-90 min area under curve) insulin (24% and 17% respectively) and C-peptide (15% and 14% respectively) responses to glucose, while the within-subject variation for the fasting values and the response to glucagon was higher (p<0.05) for insulin (47% and 32% respectively) than C-peptide (13% and 14% respectively). Between-subject variation was also lower (p<0.001) for C-peptide than for insulin. Thus, C-peptide measurements in healthy subjects are more reproducible than insulin measurements in determination of beta-cell function.</p>}},
author = {{Gottsäter, A. and Landin-Olsson, M. and Fernlund, P. and Gullberg, B. and Lernmark, Å and Sundkvist, G.}},
issn = {{0036-5513}},
keywords = {{Glucagon stimulation; Glucose tolerance test; Reproducibility}},
language = {{eng}},
month = {{01}},
number = {{7}},
pages = {{631--639}},
publisher = {{Informa Healthcare}},
series = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
title = {{Pancreatic beta-cell function evaluated by intravenous glucose and glucagon stimulation. A comparison between insulin and c-peptide to measure insulin secretion}},
url = {{http://dx.doi.org/10.3109/00365519209115506}},
doi = {{10.3109/00365519209115506}},
volume = {{52}},
year = {{1992}},
}