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Rapid and efficient induction of functional astrocytes from human pluripotent stem cells

Canals, Isaac LU ; Ginisty, Aurélie LU ; Quist, Ella LU ; Timmerman, Raissa ; Fritze, Jonas LU ; Miskinyte, Giedre LU ; Monni, Emanuela LU ; Hansen, Marita G. ; Hidalgo, Isabel LU and Bryder, David LU , et al. (2018) In Nature Methods 15(9). p.693-696
Abstract

The derivation of astrocytes from human pluripotent stem cells is currently slow and inefficient. We demonstrate that overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells rapidly and efficiently yields homogeneous populations of induced astrocytes. In our study these cells exhibited molecular and functional properties resembling those of adult human astrocytes and were deemed suitable for disease modeling. Our method provides new possibilities for the study of human astrocytes in health and disease.

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publication status
published
subject
in
Nature Methods
volume
15
issue
9
pages
4 pages
publisher
Nature Publishing Group
external identifiers
  • pmid:30127505
  • scopus:85052608291
ISSN
1548-7091
DOI
10.1038/s41592-018-0103-2
language
English
LU publication?
yes
id
dbaab1e1-8275-4799-befd-f4f06c5cc05e
date added to LUP
2018-09-25 12:29:42
date last changed
2020-03-24 06:22:40
@article{dbaab1e1-8275-4799-befd-f4f06c5cc05e,
  abstract     = {<p>The derivation of astrocytes from human pluripotent stem cells is currently slow and inefficient. We demonstrate that overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells rapidly and efficiently yields homogeneous populations of induced astrocytes. In our study these cells exhibited molecular and functional properties resembling those of adult human astrocytes and were deemed suitable for disease modeling. Our method provides new possibilities for the study of human astrocytes in health and disease.</p>},
  author       = {Canals, Isaac and Ginisty, Aurélie and Quist, Ella and Timmerman, Raissa and Fritze, Jonas and Miskinyte, Giedre and Monni, Emanuela and Hansen, Marita G. and Hidalgo, Isabel and Bryder, David and Bengzon, Johan and Ahlenius, Henrik},
  issn         = {1548-7091},
  language     = {eng},
  month        = {08},
  number       = {9},
  pages        = {693--696},
  publisher    = {Nature Publishing Group},
  series       = {Nature Methods},
  title        = {Rapid and efficient induction of functional astrocytes from human pluripotent stem cells},
  url          = {http://dx.doi.org/10.1038/s41592-018-0103-2},
  doi          = {10.1038/s41592-018-0103-2},
  volume       = {15},
  year         = {2018},
}