Rapid and efficient induction of functional astrocytes from human pluripotent stem cells
Canals, Isaac LU ; Ginisty, Aurélie LU ; Quist, Ella LU
; Timmerman, Raissa
; Fritze, Jonas
LU
; Miskinyte, Giedre
LU
; Monni, Emanuela
LU
; Hansen, Marita G.
; Hidalgo, Isabel
LU
and Bryder, David
LU
, et al.
(2018)
In Nature Methods
15(9).
p.693-696
- Abstract
The derivation of astrocytes from human pluripotent stem cells is currently slow and inefficient. We demonstrate that overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells rapidly and efficiently yields homogeneous populations of induced astrocytes. In our study these cells exhibited molecular and functional properties resembling those of adult human astrocytes and were deemed suitable for disease modeling. Our method provides new possibilities for the study of human astrocytes in health and disease.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/dbaab1e1-8275-4799-befd-f4f06c5cc05e
- author
- Canals, Isaac
LU
; Ginisty, Aurélie
LU
; Quist, Ella
LU
; Timmerman, Raissa
; Fritze, Jonas
LU
; Miskinyte, Giedre
LU
; Monni, Emanuela
LU
; Hansen, Marita G.
; Hidalgo, Isabel
LU
and Bryder, David
LU
, et al. (More)
- Canals, Isaac
LU
; Ginisty, Aurélie
LU
; Quist, Ella
LU
; Timmerman, Raissa
; Fritze, Jonas
LU
; Miskinyte, Giedre
LU
; Monni, Emanuela
LU
; Hansen, Marita G.
; Hidalgo, Isabel
LU
; Bryder, David
LU
; Bengzon, Johan
LU
and Ahlenius, Henrik
LU
(Less)
- organization
- publishing date
- 2018-08-20
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Methods
- volume
- 15
- issue
- 9
- pages
- 4 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85052608291
- pmid:30127505
- ISSN
- 1548-7091
- DOI
- 10.1038/s41592-018-0103-2
- language
- English
- LU publication?
- yes
- id
- dbaab1e1-8275-4799-befd-f4f06c5cc05e
- date added to LUP
- 2018-09-25 12:29:42
- date last changed
- 2024-06-11 20:34:00
@article{dbaab1e1-8275-4799-befd-f4f06c5cc05e,
abstract = {{<p>The derivation of astrocytes from human pluripotent stem cells is currently slow and inefficient. We demonstrate that overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells rapidly and efficiently yields homogeneous populations of induced astrocytes. In our study these cells exhibited molecular and functional properties resembling those of adult human astrocytes and were deemed suitable for disease modeling. Our method provides new possibilities for the study of human astrocytes in health and disease.</p>}},
author = {{Canals, Isaac and Ginisty, Aurélie and Quist, Ella and Timmerman, Raissa and Fritze, Jonas and Miskinyte, Giedre and Monni, Emanuela and Hansen, Marita G. and Hidalgo, Isabel and Bryder, David and Bengzon, Johan and Ahlenius, Henrik}},
issn = {{1548-7091}},
language = {{eng}},
month = {{08}},
number = {{9}},
pages = {{693--696}},
publisher = {{Nature Publishing Group}},
series = {{Nature Methods}},
title = {{Rapid and efficient induction of functional astrocytes from human pluripotent stem cells}},
url = {{http://dx.doi.org/10.1038/s41592-018-0103-2}},
doi = {{10.1038/s41592-018-0103-2}},
volume = {{15}},
year = {{2018}},
}