Characterization of the Lipid Droplet Proteome of a Clonal Insulin-producing β-Cell Line (INS-1 832/13).
Larsson, Sara LU ; Resjö, Svante LU ; Gomez, Maria LU
; James, Peter
and Holm, Cecilia
LU
(2012)
In Journal of Proteome Research
11(2).
p.1264-1273
- Abstract
- Lipids are known to play a crucial role both in the normal control of insulin release and in the deterioration of β-cell function, as observed in type 2 diabetes. Despite this established dual role of lipids, little is known about lipid storage and handling in β-cells. Here, we isolated lipid droplets from oleate-incubated INS-1 832/13 cells and characterized the lipid droplet proteome. In a total of four rounds of droplet isolation and proteomic analysis by HPLC-MS/MS, we identified 96 proteins that were specific to droplets. The proteins fall into six categories based on function or previously observed localization: metabolism, endoplasmic reticulum/ribosomes, mitochondria, vesicle formation and transport, signaling, and miscellaneous.... (More)
- Lipids are known to play a crucial role both in the normal control of insulin release and in the deterioration of β-cell function, as observed in type 2 diabetes. Despite this established dual role of lipids, little is known about lipid storage and handling in β-cells. Here, we isolated lipid droplets from oleate-incubated INS-1 832/13 cells and characterized the lipid droplet proteome. In a total of four rounds of droplet isolation and proteomic analysis by HPLC-MS/MS, we identified 96 proteins that were specific to droplets. The proteins fall into six categories based on function or previously observed localization: metabolism, endoplasmic reticulum/ribosomes, mitochondria, vesicle formation and transport, signaling, and miscellaneous. The protein profile reinforces the emerging picture of the lipid droplet as an active and dynamic organelle involved in lipid homeostasis and intracellular trafficking. Proteins belonging to the category mitochondria were highly represented, suggesting that the β-cell mitochondria and lipid droplets form a metabolic unit of potential relevance for insulin secretion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2336060
- author
- Larsson, Sara
LU
; Resjö, Svante
LU
; Gomez, Maria
LU
; James, Peter
and Holm, Cecilia
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- insulin, proteomics, lipid droplets, beta-cells
- in
- Journal of Proteome Research
- volume
- 11
- issue
- 2
- pages
- 1264 - 1273
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000300458300064
- pmid:22268682
- scopus:84856656225
- ISSN
- 1535-3893
- DOI
- 10.1021/pr200957p
- language
- English
- LU publication?
- yes
- id
- e3354c22-f260-44b1-acc8-24b207192dff (old id 2336060)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22268682?dopt=Abstract
- date added to LUP
- 2016-04-01 11:05:12
- date last changed
- 2022-01-26 05:16:25
@article{e3354c22-f260-44b1-acc8-24b207192dff,
abstract = {{Lipids are known to play a crucial role both in the normal control of insulin release and in the deterioration of β-cell function, as observed in type 2 diabetes. Despite this established dual role of lipids, little is known about lipid storage and handling in β-cells. Here, we isolated lipid droplets from oleate-incubated INS-1 832/13 cells and characterized the lipid droplet proteome. In a total of four rounds of droplet isolation and proteomic analysis by HPLC-MS/MS, we identified 96 proteins that were specific to droplets. The proteins fall into six categories based on function or previously observed localization: metabolism, endoplasmic reticulum/ribosomes, mitochondria, vesicle formation and transport, signaling, and miscellaneous. The protein profile reinforces the emerging picture of the lipid droplet as an active and dynamic organelle involved in lipid homeostasis and intracellular trafficking. Proteins belonging to the category mitochondria were highly represented, suggesting that the β-cell mitochondria and lipid droplets form a metabolic unit of potential relevance for insulin secretion.}},
author = {{Larsson, Sara and Resjö, Svante and Gomez, Maria and James, Peter and Holm, Cecilia}},
issn = {{1535-3893}},
keywords = {{insulin; proteomics; lipid droplets; beta-cells}},
language = {{eng}},
number = {{2}},
pages = {{1264--1273}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of Proteome Research}},
title = {{Characterization of the Lipid Droplet Proteome of a Clonal Insulin-producing β-Cell Line (INS-1 832/13).}},
url = {{http://dx.doi.org/10.1021/pr200957p}},
doi = {{10.1021/pr200957p}},
volume = {{11}},
year = {{2012}},
}