Omics Analyses Reveal a Potential Link between Hormone-Sensitive Lipase and Polyamine Metabolism.
Fernandez, Céline ; Krogh, Morten LU ; Wårell, Kristofer LU ; Alm, Kersti LU ; Oredsson, Stina LU ; Persson, Lo LU ; James, Peter LU
and Holm, Cecilia
LU
(2009)
In Journal of Proteome Research
8.
p.5008-5019
- Abstract
- Hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization from lipid stores, is expressed in the liver and decreased hepatic insulin sensitivity has been reported in our HSL null mouse model. Here, an integrated approach, comprising transcriptomics and proteomics together with targeted metabolite analysis, was used to investigate the liver phenotype of HSL null mice. Oligonucleotide microarray analysis revealed altered expression of genes involved in lipid and polyamine metabolism in HSL null mice compared with wild-type mice and in genes controlling the immune system in mice on high-fat diet versus mice on normal diet. Two-dimensional gel electrophoresis followed by MS and/or MS/MS allowed identification of 52 and 22 unique... (More)
- Hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization from lipid stores, is expressed in the liver and decreased hepatic insulin sensitivity has been reported in our HSL null mouse model. Here, an integrated approach, comprising transcriptomics and proteomics together with targeted metabolite analysis, was used to investigate the liver phenotype of HSL null mice. Oligonucleotide microarray analysis revealed altered expression of genes involved in lipid and polyamine metabolism in HSL null mice compared with wild-type mice and in genes controlling the immune system in mice on high-fat diet versus mice on normal diet. Two-dimensional gel electrophoresis followed by MS and/or MS/MS allowed identification of 52 and 22 unique proteins differentially regulated according to the genotype and diet, respectively. Changes were observed mainly for proteins related to metabolism, including several proteins involved in polyamine metabolism or exhibiting methyl transferase activity. Despite the coordinated changes in mRNA and protein levels in polyamine pathways, no significant differences in levels of key polyamine metabolites were detected between the two genotypes. This study identifies a link between HSL and polyamine metabolism, which deserves further attention in view of the emerging data suggesting that disturbances in polyamine metabolism may affect insulin sensitivity. The present work also describes a limited correlation between mRNA, protein and metabolite levels, thus, underscoring the importance of integrated approaches. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1500954
- author
- Fernandez, Céline
; Krogh, Morten
LU
; Wårell, Kristofer
LU
; Alm, Kersti
LU
; Oredsson, Stina
LU
; Persson, Lo
LU
; James, Peter
LU
and Holm, Cecilia
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Proteome Research
- volume
- 8
- pages
- 5008 - 5019
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000276949600010
- pmid:19785415
- scopus:70449360655
- pmid:19785415
- ISSN
- 1535-3893
- DOI
- 10.1021/pr9004037
- language
- English
- LU publication?
- yes
- id
- e672da25-2605-41a9-9077-976e74c8ebfb (old id 1500954)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19785415?dopt=Abstract
- date added to LUP
- 2016-04-04 07:10:57
- date last changed
- 2023-09-05 11:09:25
@article{e672da25-2605-41a9-9077-976e74c8ebfb,
abstract = {{Hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization from lipid stores, is expressed in the liver and decreased hepatic insulin sensitivity has been reported in our HSL null mouse model. Here, an integrated approach, comprising transcriptomics and proteomics together with targeted metabolite analysis, was used to investigate the liver phenotype of HSL null mice. Oligonucleotide microarray analysis revealed altered expression of genes involved in lipid and polyamine metabolism in HSL null mice compared with wild-type mice and in genes controlling the immune system in mice on high-fat diet versus mice on normal diet. Two-dimensional gel electrophoresis followed by MS and/or MS/MS allowed identification of 52 and 22 unique proteins differentially regulated according to the genotype and diet, respectively. Changes were observed mainly for proteins related to metabolism, including several proteins involved in polyamine metabolism or exhibiting methyl transferase activity. Despite the coordinated changes in mRNA and protein levels in polyamine pathways, no significant differences in levels of key polyamine metabolites were detected between the two genotypes. This study identifies a link between HSL and polyamine metabolism, which deserves further attention in view of the emerging data suggesting that disturbances in polyamine metabolism may affect insulin sensitivity. The present work also describes a limited correlation between mRNA, protein and metabolite levels, thus, underscoring the importance of integrated approaches.}},
author = {{Fernandez, Céline and Krogh, Morten and Wårell, Kristofer and Alm, Kersti and Oredsson, Stina and Persson, Lo and James, Peter and Holm, Cecilia}},
issn = {{1535-3893}},
language = {{eng}},
pages = {{5008--5019}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of Proteome Research}},
title = {{Omics Analyses Reveal a Potential Link between Hormone-Sensitive Lipase and Polyamine Metabolism.}},
url = {{http://dx.doi.org/10.1021/pr9004037}},
doi = {{10.1021/pr9004037}},
volume = {{8}},
year = {{2009}},
}