Oncostatin M signaling in human glioma cell lines
(2005) In Oncology Reports 13(5). p.807-811- Abstract
- We have recently found that oncostatin M (OSM) is overexpressed in most human brain tumors. The effects of OSM are unclear with conflicting reports of growth stimulatory or inhibitory effects in various cell types. The aim of this study was to investigate the effects of OSM in 5 glioma cell lines and 7 short-term cultures of human gliomas and in normal cultured human astrocytes. None of the cell lines and short-term cultured tumor cells expressed OSM in vitro. OSM signals through a gp130 containing receptor complex over the JAK/STAT pathway. Immunofluorescence and RT-PCR analysis showed that the tumor cells express gp130 and the other receptor components, LIFR beta and OSMR beta. OSM treatment induced phosphorylation of STAT3 and STAT1... (More)
- We have recently found that oncostatin M (OSM) is overexpressed in most human brain tumors. The effects of OSM are unclear with conflicting reports of growth stimulatory or inhibitory effects in various cell types. The aim of this study was to investigate the effects of OSM in 5 glioma cell lines and 7 short-term cultures of human gliomas and in normal cultured human astrocytes. None of the cell lines and short-term cultured tumor cells expressed OSM in vitro. OSM signals through a gp130 containing receptor complex over the JAK/STAT pathway. Immunofluorescence and RT-PCR analysis showed that the tumor cells express gp130 and the other receptor components, LIFR beta and OSMR beta. OSM treatment induced phosphorylation of STAT3 and STAT1 indicating presence of a functional JAK/STAT pathway. No OSM effect on proliferation was observed. OSM gave no protective effects against tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced cytotoxicity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/246339
- author
- Krona, A ; Järnum, Sofia LU ; Salford, Leif LU ; Widegren, Bengt LU and Aman, P
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cytotoxicity, STAT3, proliferation, oncostatin M, astrocytoma, brain tumor
- in
- Oncology Reports
- volume
- 13
- issue
- 5
- pages
- 807 - 811
- publisher
- Spandidos Publications
- external identifiers
-
- pmid:15809742
- wos:000228440500004
- scopus:25144517324
- ISSN
- 1791-2431
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100), Clinic of Neurosurgery: University Hospital, Lund (LUR000009), Department of Experimental Medical Science (013210000), Division IV (013230800)
- id
- e92327fb-85b9-4113-b55d-a22fe8b5da64 (old id 246339)
- alternative location
- http://147.52.72.117/OR/2005/volume13/number5/807.pdf
- date added to LUP
- 2016-04-01 12:03:45
- date last changed
- 2022-01-26 22:13:01
@article{e92327fb-85b9-4113-b55d-a22fe8b5da64, abstract = {{We have recently found that oncostatin M (OSM) is overexpressed in most human brain tumors. The effects of OSM are unclear with conflicting reports of growth stimulatory or inhibitory effects in various cell types. The aim of this study was to investigate the effects of OSM in 5 glioma cell lines and 7 short-term cultures of human gliomas and in normal cultured human astrocytes. None of the cell lines and short-term cultured tumor cells expressed OSM in vitro. OSM signals through a gp130 containing receptor complex over the JAK/STAT pathway. Immunofluorescence and RT-PCR analysis showed that the tumor cells express gp130 and the other receptor components, LIFR beta and OSMR beta. OSM treatment induced phosphorylation of STAT3 and STAT1 indicating presence of a functional JAK/STAT pathway. No OSM effect on proliferation was observed. OSM gave no protective effects against tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced cytotoxicity.}}, author = {{Krona, A and Järnum, Sofia and Salford, Leif and Widegren, Bengt and Aman, P}}, issn = {{1791-2431}}, keywords = {{cytotoxicity; STAT3; proliferation; oncostatin M; astrocytoma; brain tumor}}, language = {{eng}}, number = {{5}}, pages = {{807--811}}, publisher = {{Spandidos Publications}}, series = {{Oncology Reports}}, title = {{Oncostatin M signaling in human glioma cell lines}}, url = {{http://147.52.72.117/OR/2005/volume13/number5/807.pdf}}, volume = {{13}}, year = {{2005}}, }