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Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry

Rolandsson Enes, Sara LU ; Åhrman, Emma LU ; Palani, Anitha; Hallgren, Oskar LU ; Bjermer, Leif LU ; Malmström, Anders LU ; Scheding, Stefan LU ; Malmström, Johan LU and Westergren-Thorsson, Gunilla LU (2017) In Scientific Reports 7(1).
Abstract

Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We... (More)

Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We identified several significantly differentially expressed proteins between lung-MSC and bone marrow-MSC within the cell layer (352 proteins) and in the conditioned medium (49 proteins). Bioinformatics analysis revealed differences in regulation of cell proliferation, which was functionally confirmed by decreasing proliferation rate through Cytochrome P450 stimulation. Our study reveals important differences within proteome and matrisome profiles between lung- and bone marrow-derived MSC that may influence their behavior and affect the clinical outcome when used for cell-therapy.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Journal Article
in
Scientific Reports
volume
7
issue
1
publisher
Nature Publishing Group
external identifiers
  • scopus:85028076535
  • wos:000408441600040
ISSN
2045-2322
DOI
10.1038/s41598-017-09127-y
language
English
LU publication?
yes
id
eee65450-6f88-4664-a5ca-1ab378efe401
date added to LUP
2017-09-04 17:11:06
date last changed
2017-09-18 11:38:50
@article{eee65450-6f88-4664-a5ca-1ab378efe401,
  abstract     = {<p>Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We identified several significantly differentially expressed proteins between lung-MSC and bone marrow-MSC within the cell layer (352 proteins) and in the conditioned medium (49 proteins). Bioinformatics analysis revealed differences in regulation of cell proliferation, which was functionally confirmed by decreasing proliferation rate through Cytochrome P450 stimulation. Our study reveals important differences within proteome and matrisome profiles between lung- and bone marrow-derived MSC that may influence their behavior and affect the clinical outcome when used for cell-therapy.</p>},
  articleno    = {9316},
  author       = {Rolandsson Enes, Sara and Åhrman, Emma and Palani, Anitha and Hallgren, Oskar and Bjermer, Leif and Malmström, Anders and Scheding, Stefan and Malmström, Johan and Westergren-Thorsson, Gunilla},
  issn         = {2045-2322},
  keyword      = {Journal Article},
  language     = {eng},
  month        = {08},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry},
  url          = {http://dx.doi.org/10.1038/s41598-017-09127-y},
  volume       = {7},
  year         = {2017},
}