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On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes

Kjellström, S LU ; Emnéus, J LU ; Laurell, T LU ; Heintz, L and Marko-Varga, G LU (1998) In Journal of chromatography. A 823(1-2). p.489-496
Abstract

An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perfusion liquid used for sampling hydrophobic eicosanoids in biological systems was also studied. The total time for one analysis was 7.6 min allowing seven measurements per hour for monitoring kinetic changes in biological systems.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Autoanalysis, Cell Line, Chromatography, Liquid/instrumentation, Culture Media, Conditioned, Cyclodextrins, Eicosanoids/analysis, Humans, Hydrogen-Ion Concentration, Indicators and Reagents, Leukotriene B4/analysis, Leukotriene C4/analysis, Leukotriene D4/analysis, Leukotriene E4/analysis, Leukotrienes/analysis, Microdialysis/instrumentation, Monocytes/metabolism, Prostaglandins B/analysis
in
Journal of chromatography. A
volume
823
issue
1-2
pages
8 pages
publisher
Elsevier
external identifiers
  • pmid:9818423
  • scopus:0032500515
ISSN
0021-9673
DOI
10.1016/S0021-9673(98)00361-6
language
English
LU publication?
yes
id
ef8c9e3f-4269-4ac6-b26e-db6cd895e19a
date added to LUP
2018-06-05 09:13:02
date last changed
2025-02-05 16:00:36
@article{ef8c9e3f-4269-4ac6-b26e-db6cd895e19a,
  abstract     = {{<p>An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perfusion liquid used for sampling hydrophobic eicosanoids in biological systems was also studied. The total time for one analysis was 7.6 min allowing seven measurements per hour for monitoring kinetic changes in biological systems.</p>}},
  author       = {{Kjellström, S and Emnéus, J and Laurell, T and Heintz, L and Marko-Varga, G}},
  issn         = {{0021-9673}},
  keywords     = {{Autoanalysis; Cell Line; Chromatography, Liquid/instrumentation; Culture Media, Conditioned; Cyclodextrins; Eicosanoids/analysis; Humans; Hydrogen-Ion Concentration; Indicators and Reagents; Leukotriene B4/analysis; Leukotriene C4/analysis; Leukotriene D4/analysis; Leukotriene E4/analysis; Leukotrienes/analysis; Microdialysis/instrumentation; Monocytes/metabolism; Prostaglandins B/analysis}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{1-2}},
  pages        = {{489--496}},
  publisher    = {{Elsevier}},
  series       = {{Journal of chromatography. A}},
  title        = {{On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(98)00361-6}},
  doi          = {{10.1016/S0021-9673(98)00361-6}},
  volume       = {{823}},
  year         = {{1998}},
}