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Co-adsorption of beta-casein and calcium phosphate nanoclusters (CPN) at hydrophilic and hydrophobic solid-solution interfaces studied by neutron reflectometry

Follows, David ; Holt, Carl ; Thomas, Robert K. ; Tiberg, Fredrik LU ; Fragneto, Giovanna and Nylander, Tommy LU (2011) Conference on Food Colloids 2010 - On the Road From Interfaces to Consumers 25(4). p.724-733
Abstract
Neutron reflectometry was used to study the co-adsorption of calcium phosphate nanoclusters (CPN) and beta-casein at hydrophobized and hydrophilic silica-water interfaces. The structural characteristics of the adsorbed layer were determined from neutron reflectivity curves analysed with multi-layer optical models. We used a highly specific proteolytic enzyme, endoproteinase Asp-N in conjunction with a single neutron contrast to verify the model of the protein layer structure. The results showed that the calcium phosphate nanoclusters profoundly affected the rate of adsorption and structure of the interface compared to the adsorption of beta-casein alone and for the hydrophobic interface the effects depended on the point at which the... (More)
Neutron reflectometry was used to study the co-adsorption of calcium phosphate nanoclusters (CPN) and beta-casein at hydrophobized and hydrophilic silica-water interfaces. The structural characteristics of the adsorbed layer were determined from neutron reflectivity curves analysed with multi-layer optical models. We used a highly specific proteolytic enzyme, endoproteinase Asp-N in conjunction with a single neutron contrast to verify the model of the protein layer structure. The results showed that the calcium phosphate nanoclusters profoundly affected the rate of adsorption and structure of the interface compared to the adsorption of beta-casein alone and for the hydrophobic interface the effects depended on the point at which the nanoclusters were added. It is proposed that the nanoclusters become surface active because whole beta-casein molecules can replace one or more of the hydrophilic peptides in the shell of the nanoclusters. (C) 2010 Elsevier Ltd. All rights reserved. (Less)
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author
; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
beta-casein, Calcium phosphate nanoclusters, Adsorption, Neutron, reflectometry
host publication
Food Hydrocolloids
volume
25
issue
4
pages
724 - 733
publisher
Elsevier
conference name
Conference on Food Colloids 2010 - On the Road From Interfaces to Consumers
conference location
Granada, Spain
conference dates
2010-03-21 - 2010-03-24
external identifiers
  • wos:000287309700021
  • scopus:79851485254
ISSN
1873-7137
0268-005X
DOI
10.1016/j.foodhyd.2010.09.001
language
English
LU publication?
yes
id
f856711c-e04d-466e-a007-007e8f7c3adb (old id 1872923)
date added to LUP
2016-04-01 09:58:23
date last changed
2025-04-04 15:19:16
@inproceedings{f856711c-e04d-466e-a007-007e8f7c3adb,
  abstract     = {{Neutron reflectometry was used to study the co-adsorption of calcium phosphate nanoclusters (CPN) and beta-casein at hydrophobized and hydrophilic silica-water interfaces. The structural characteristics of the adsorbed layer were determined from neutron reflectivity curves analysed with multi-layer optical models. We used a highly specific proteolytic enzyme, endoproteinase Asp-N in conjunction with a single neutron contrast to verify the model of the protein layer structure. The results showed that the calcium phosphate nanoclusters profoundly affected the rate of adsorption and structure of the interface compared to the adsorption of beta-casein alone and for the hydrophobic interface the effects depended on the point at which the nanoclusters were added. It is proposed that the nanoclusters become surface active because whole beta-casein molecules can replace one or more of the hydrophilic peptides in the shell of the nanoclusters. (C) 2010 Elsevier Ltd. All rights reserved.}},
  author       = {{Follows, David and Holt, Carl and Thomas, Robert K. and Tiberg, Fredrik and Fragneto, Giovanna and Nylander, Tommy}},
  booktitle    = {{Food Hydrocolloids}},
  issn         = {{1873-7137}},
  keywords     = {{beta-casein; Calcium phosphate nanoclusters; Adsorption; Neutron; reflectometry}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{724--733}},
  publisher    = {{Elsevier}},
  title        = {{Co-adsorption of beta-casein and calcium phosphate nanoclusters (CPN) at hydrophilic and hydrophobic solid-solution interfaces studied by neutron reflectometry}},
  url          = {{http://dx.doi.org/10.1016/j.foodhyd.2010.09.001}},
  doi          = {{10.1016/j.foodhyd.2010.09.001}},
  volume       = {{25}},
  year         = {{2011}},
}