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Co-elution effects can influence molar mass determination of large macromolecules with asymmetric flow field-flow fractionation coupled to multiangle light scattering

Pérez-Rea, Daysi LU ; Zielke, Claudia LU and Nilsson, Lars LU (2017) In Journal of Chromatography A 1506. p.138-141
Abstract

Starch and hence, amylopectin is an important biomacromolecule in both the human diet as well as in technical applications. Therefore, accurate and reliable analytical methods for its characterization are needed. A suitable method for analyzing macromolecules with ultra-high molar mass, branched structure and high polydispersity is asymmetric flow field-flow fractionation (AF4) in combination with multiangle light scattering (MALS) detection. In this paper we illustrate how co-elution of low quantities of very large analytes in AF4 may cause disturbances in the MALS data which, in turn, causes an overestimation of the size. Furthermore, it is shown how pre-injection filtering of the sample can improve the results.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amylopectin, Asymmetric flow field-flow fractionation (AF4), Macromolecules, Multiangle light scattering (MALS), Root-mean-square radius (r), Size distribution
in
Journal of Chromatography A
volume
1506
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:85019620178
  • wos:000403990300017
ISSN
0021-9673
DOI
10.1016/j.chroma.2017.05.029
language
English
LU publication?
yes
id
fd8c4fcf-614d-42e4-8a75-b63ce263bd75
date added to LUP
2017-06-13 13:10:29
date last changed
2017-09-18 11:40:29
@article{fd8c4fcf-614d-42e4-8a75-b63ce263bd75,
  abstract     = {<p>Starch and hence, amylopectin is an important biomacromolecule in both the human diet as well as in technical applications. Therefore, accurate and reliable analytical methods for its characterization are needed. A suitable method for analyzing macromolecules with ultra-high molar mass, branched structure and high polydispersity is asymmetric flow field-flow fractionation (AF4) in combination with multiangle light scattering (MALS) detection. In this paper we illustrate how co-elution of low quantities of very large analytes in AF4 may cause disturbances in the MALS data which, in turn, causes an overestimation of the size. Furthermore, it is shown how pre-injection filtering of the sample can improve the results.</p>},
  author       = {Pérez-Rea, Daysi and Zielke, Claudia and Nilsson, Lars},
  issn         = {0021-9673},
  keyword      = {Amylopectin,Asymmetric flow field-flow fractionation (AF4),Macromolecules,Multiangle light scattering (MALS),Root-mean-square radius (r),Size distribution},
  language     = {eng},
  month        = {07},
  pages        = {138--141},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Co-elution effects can influence molar mass determination of large macromolecules with asymmetric flow field-flow fractionation coupled to multiangle light scattering},
  url          = {http://dx.doi.org/10.1016/j.chroma.2017.05.029},
  volume       = {1506},
  year         = {2017},
}