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Characterization of plasma lipidomics in adolescent subjects with increased risk for type 1 diabetes in the DiPiS cohort

Andersson Svärd, Agnes LU orcid ; Kaur, Simranjeet ; Trôst, Kajetan ; Suvitaival, Tommi ; Lernmark, Åke LU orcid ; Maziarz, Marlena LU ; Pociot, Flemming LU and Overgaard, Anne Julie (2020) In Metabolomics 16(10).
Abstract

INTRODUCTION: Type 1 diabetes (T1D) is caused by the destruction of pancreatic islet beta cells resulting in total loss of insulin production. Recent studies have suggested that the destruction may be interrelated to plasma lipids.

OBJECTIVES: Specific lipids have previously been shown to be decreased in children who develop T1D before four years of age. Disturbances of plasma lipids prior to clinical diagnosis of diabetes, if true, may provide a novel way to improve prediction, and monitor disease progression.

METHODS: A lipidomic approach was utilized to analyze plasma from 67 healthy adolescent subjects (10-15 years of age) with or without islet autoantibodies but all with increased genetic risk for T1D. The study... (More)

INTRODUCTION: Type 1 diabetes (T1D) is caused by the destruction of pancreatic islet beta cells resulting in total loss of insulin production. Recent studies have suggested that the destruction may be interrelated to plasma lipids.

OBJECTIVES: Specific lipids have previously been shown to be decreased in children who develop T1D before four years of age. Disturbances of plasma lipids prior to clinical diagnosis of diabetes, if true, may provide a novel way to improve prediction, and monitor disease progression.

METHODS: A lipidomic approach was utilized to analyze plasma from 67 healthy adolescent subjects (10-15 years of age) with or without islet autoantibodies but all with increased genetic risk for T1D. The study subjects were enrolled at birth in the Diabetes Prediction in Skåne (DiPiS) study and after 10-15 years of follow-up we performed the present cross-sectional analysis. HLA-DRB345, -DRB1, -DQA1, -DQB1, -DPA1 and -DPB1 genotypes were determined using next generation sequencing. Lipidomic profiles were determined using ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry. Lipidomics data were analyzed according to genotype.

RESULTS: Variation in levels of several specific phospholipid species were related to level of autoimmunity but not development of T1D. Five glycosylated ceramides were increased in insulin autoantibody (IAA) positive adolescent subjects compared to adolescent subjects without this autoantibody. Additionally, HLA genotypes seemed to influence levels of long chain triacylglycerol (TG).

CONCLUSION: Lipidomic profiling of adolescent subjects in high risk of T1D may improve sub-phenotyping in this high risk population.

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author
; ; ; ; ; ; and
author collaboration
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Metabolomics
volume
16
issue
10
article number
109
publisher
Springer
external identifiers
  • scopus:85092684627
  • pmid:33033923
ISSN
1573-3882
DOI
10.1007/s11306-020-01730-x
project
Immunological markers of type 1 diabetes pathogenesis prior to clinical diagnosis
language
English
LU publication?
yes
additional info
Agnes Andersson Svärd and Anne Julie Overgaard have contributed equally to this study.
id
fdf6a555-5e4f-465a-af85-2e6536777d25
date added to LUP
2020-10-14 07:59:38
date last changed
2024-04-03 15:59:12
@article{fdf6a555-5e4f-465a-af85-2e6536777d25,
  abstract     = {{<p>INTRODUCTION: Type 1 diabetes (T1D) is caused by the destruction of pancreatic islet beta cells resulting in total loss of insulin production. Recent studies have suggested that the destruction may be interrelated to plasma lipids.</p><p>OBJECTIVES: Specific lipids have previously been shown to be decreased in children who develop T1D before four years of age. Disturbances of plasma lipids prior to clinical diagnosis of diabetes, if true, may provide a novel way to improve prediction, and monitor disease progression.</p><p>METHODS: A lipidomic approach was utilized to analyze plasma from 67 healthy adolescent subjects (10-15 years of age) with or without islet autoantibodies but all with increased genetic risk for T1D. The study subjects were enrolled at birth in the Diabetes Prediction in Skåne (DiPiS) study and after 10-15 years of follow-up we performed the present cross-sectional analysis. HLA-DRB345, -DRB1, -DQA1, -DQB1, -DPA1 and -DPB1 genotypes were determined using next generation sequencing. Lipidomic profiles were determined using ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry. Lipidomics data were analyzed according to genotype.</p><p>RESULTS: Variation in levels of several specific phospholipid species were related to level of autoimmunity but not development of T1D. Five glycosylated ceramides were increased in insulin autoantibody (IAA) positive adolescent subjects compared to adolescent subjects without this autoantibody. Additionally, HLA genotypes seemed to influence levels of long chain triacylglycerol (TG).</p><p>CONCLUSION: Lipidomic profiling of adolescent subjects in high risk of T1D may improve sub-phenotyping in this high risk population.</p>}},
  author       = {{Andersson Svärd, Agnes and Kaur, Simranjeet and Trôst, Kajetan and Suvitaival, Tommi and Lernmark, Åke and Maziarz, Marlena and Pociot, Flemming and Overgaard, Anne Julie}},
  issn         = {{1573-3882}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{10}},
  publisher    = {{Springer}},
  series       = {{Metabolomics}},
  title        = {{Characterization of plasma lipidomics in adolescent subjects with increased risk for type 1 diabetes in the DiPiS cohort}},
  url          = {{http://dx.doi.org/10.1007/s11306-020-01730-x}},
  doi          = {{10.1007/s11306-020-01730-x}},
  volume       = {{16}},
  year         = {{2020}},
}