Geobacillus 7349 – A new potential biosurfactant producing microorganism
(2023) KBTM01 20231Biotechnology (MSc)
Biotechnology (M.Sc.Eng.)
- Abstract
- The main aim of this master project is to find out whether strain Geobacillus 7349 can produce biosurfactant during their growth by extraction and characterization of the product. The other goal is to optimize the cultivation condition of the strain. This work presents an evaluation of batch and fed-batch cultivation techniques for production of Geobacillus 7349 in shake flask, using DRM medium. The growth condition of the strain was monitored by cell density (OD) measurement, cell dry weight measurement, along with glucose consumption characterized by HPAEC-PAD. Batch cultivation results in a high cell density (OD600 ≥ 5) after around 14 hours of cultivation and a high concentration of biosurfactant being produced. To extract the... (More)
- The main aim of this master project is to find out whether strain Geobacillus 7349 can produce biosurfactant during their growth by extraction and characterization of the product. The other goal is to optimize the cultivation condition of the strain. This work presents an evaluation of batch and fed-batch cultivation techniques for production of Geobacillus 7349 in shake flask, using DRM medium. The growth condition of the strain was monitored by cell density (OD) measurement, cell dry weight measurement, along with glucose consumption characterized by HPAEC-PAD. Batch cultivation results in a high cell density (OD600 ≥ 5) after around 14 hours of cultivation and a high concentration of biosurfactant being produced. To extract the biosurfactant from the culture broth, liquid-liquid extraction is an effective method. In both batch and fed-batch cultivations, the production of biosurfactant was detectable in both exponential and stationary phase, by the usage of high-performance liquid chromatography (HPLC), which means the cells were active in producing biosurfactant during these phases. And the fed-batch cultivation supplemented with fresh feed solution containing MgCl2, NH4Cl, and NaCl can increase the cell density threshold and prolong the lifespan of cell generation. To conclude, the project demonstrates the production of biosurfactant from strain Geobacillus 7349 by fermentation and the importance of the process design and nutrient source for bacterial growth. Hopefully, these findings can be used to scale up the fermentation process of Geobacillus 7349 from lab scale to trial scale, in order to have a more substantial biosurfactant production. (Less)
- Popular Abstract
- Surfactant is widely used in many industrial fields such as petroleum, food, pharmaceuticals and so on as detergent, solubilizers or emulsifying agents. Currently, most of the surfactants are chemical petroleum based due to low cost but they are often toxic, irritant and non-biodegradable. Furthermore, petroleum for chemical synthesis of surfactant isn’t renewable. People are trying to reduce their dependence on fossil fuels and move forward to a more renewable source. And biosurfactants which are produced by microorganisms as secondary metabolites, typically bacteria and yeast, become environmental friendly alternatives, as they are usually more biodegradable and less toxic than petroleum derived ones (Jyoti Sharma 2021). Biosurfactant... (More)
- Surfactant is widely used in many industrial fields such as petroleum, food, pharmaceuticals and so on as detergent, solubilizers or emulsifying agents. Currently, most of the surfactants are chemical petroleum based due to low cost but they are often toxic, irritant and non-biodegradable. Furthermore, petroleum for chemical synthesis of surfactant isn’t renewable. People are trying to reduce their dependence on fossil fuels and move forward to a more renewable source. And biosurfactants which are produced by microorganisms as secondary metabolites, typically bacteria and yeast, become environmental friendly alternatives, as they are usually more biodegradable and less toxic than petroleum derived ones (Jyoti Sharma 2021). Biosurfactant can be divided into various categories. Among them, rhamnolipids are the most popularly researched one in recent years. However, production of rhamnolipids by microbial fermentation is usually very costly, due to the high substrate cost and high production cost. The carbon sources used for production are preferably cheap, such as glucose in our case. In addition, reducing the cost of downstream processing is another essential strategy to reduce the production cost. The extraction of rhamnolipids can make up 80% of the total production cost. The complexity of the extraction depends on the selected strain and the produced culture mixture and it shouldn’t be over complicated.
There’re quite a few strains producing rhamnolipids, nowadays. In this project, Geobacillus 7349 is the strain used to investigate the possibility of producing biosurfactant. Geobacillus, is gram-positive, rod shaped, aerobic species that is isolated from hot spring in Iceland. It can grow within a temperature range of 30 to 75 °C (Burgess, Flint et al. 2017). And it is naturally selected, without making any genetic modification. In this report, the cultivation of Geobacillus 7349 in a defined medium and the extraction and characterization of the product are the two main concerns. On one hand, it’s important to define the culture condition of the strain. On the other hand, it’s vital to extract and characterize our product, in order to know what is produced. The results can help with the further development of the production process of biosurfactant by Geobacillus 7349 in larger scale. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9124499
- author
- Deng, Zhuoying LU
- supervisor
- organization
- course
- KBTM01 20231
- year
- 2023
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- biosurfactant, rhamnolipids, HPLC, HPAEC-PAD, bacteria growth, downstream purification, liquid-liquid extraction, biotechnology
- language
- English
- id
- 9124499
- date added to LUP
- 2023-06-14 10:05:33
- date last changed
- 2023-06-14 10:05:33
@misc{9124499,
abstract = {{The main aim of this master project is to find out whether strain Geobacillus 7349 can produce biosurfactant during their growth by extraction and characterization of the product. The other goal is to optimize the cultivation condition of the strain. This work presents an evaluation of batch and fed-batch cultivation techniques for production of Geobacillus 7349 in shake flask, using DRM medium. The growth condition of the strain was monitored by cell density (OD) measurement, cell dry weight measurement, along with glucose consumption characterized by HPAEC-PAD. Batch cultivation results in a high cell density (OD600 ≥ 5) after around 14 hours of cultivation and a high concentration of biosurfactant being produced. To extract the biosurfactant from the culture broth, liquid-liquid extraction is an effective method. In both batch and fed-batch cultivations, the production of biosurfactant was detectable in both exponential and stationary phase, by the usage of high-performance liquid chromatography (HPLC), which means the cells were active in producing biosurfactant during these phases. And the fed-batch cultivation supplemented with fresh feed solution containing MgCl2, NH4Cl, and NaCl can increase the cell density threshold and prolong the lifespan of cell generation. To conclude, the project demonstrates the production of biosurfactant from strain Geobacillus 7349 by fermentation and the importance of the process design and nutrient source for bacterial growth. Hopefully, these findings can be used to scale up the fermentation process of Geobacillus 7349 from lab scale to trial scale, in order to have a more substantial biosurfactant production.}},
author = {{Deng, Zhuoying}},
language = {{eng}},
note = {{Student Paper}},
title = {{Geobacillus 7349 – A new potential biosurfactant producing microorganism}},
year = {{2023}},
}