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Stability screening of enzyme formulation in solid format

Andersson, Erika LU (2024) KBTM05 20241
Biotechnology
Biotechnology (MSc)
Abstract
Enzyme formulation development is a tedious process that requires lots of time and resources.
By improving parts of the formulation process, the formulation of enzymes could become more
efficient and allow for quicker commercialization of enzyme products. The focus of this project
has been on solid granular enzyme formulations, aiming to accelerate stability studies, develop
enzyme degradation prediction models and validate a small-scale screening system. The three
feed enzymes of phytase, amylase and protease were subjected to varying time, temperature,
pH and humidity to then be analyzed using mainly nanoDSF and activity assays. The results
helped widen the understanding of enzyme degradation caused by these various storage
... (More)
Enzyme formulation development is a tedious process that requires lots of time and resources.
By improving parts of the formulation process, the formulation of enzymes could become more
efficient and allow for quicker commercialization of enzyme products. The focus of this project
has been on solid granular enzyme formulations, aiming to accelerate stability studies, develop
enzyme degradation prediction models and validate a small-scale screening system. The three
feed enzymes of phytase, amylase and protease were subjected to varying time, temperature,
pH and humidity to then be analyzed using mainly nanoDSF and activity assays. The results
helped widen the understanding of enzyme degradation caused by these various storage
conditions and prediction of enzyme stability has been validated, while also showing that high
enzyme stability and high unfolding temperatures do not always correlate. The humidity has a
substantial effect on enzyme stability. Also, the possibility of accelerating stability studies
remains uncertain. Lastly, a small scale screening system has been successfully developed. (Less)
Popular Abstract
The application of enzymes range a broad spectrum, from detergents and textile processing to
food and feed. One such important application is feed enzymes utilized in animal feed to enable
improved nutrient uptake for animals. When the nutrient uptake becomes more successful, the
animals naturally eat less, lowering the economic and environmental footprint. These enzymes
are vital parts of animal feed products and play an important role in industry.
Enzyme stability is an important quality parameter for products containing active enzymes. The
stability of the enzyme is essential to ensure that the desired activity can be delivered in the
application. The enzyme stability can be improved or facilitated through the right formulation
... (More)
The application of enzymes range a broad spectrum, from detergents and textile processing to
food and feed. One such important application is feed enzymes utilized in animal feed to enable
improved nutrient uptake for animals. When the nutrient uptake becomes more successful, the
animals naturally eat less, lowering the economic and environmental footprint. These enzymes
are vital parts of animal feed products and play an important role in industry.
Enzyme stability is an important quality parameter for products containing active enzymes. The
stability of the enzyme is essential to ensure that the desired activity can be delivered in the
application. The enzyme stability can be improved or facilitated through the right formulation
of ingredients. Formulation development is a tedious process requiring lots of time, resources,
and money. Naturally, there are wishes to improve this procedure.
The three pillars to improve formulation development in this project are acceleration of
stability studies to obtain stability data faster, increase knowledge of enzyme degradation to
create empirical stability prediction models and lastly, preparation of small scale formulations
to enable higher throughput screening of formulation candidates or engineered protein
candidates.
The studied enzymes were phytase, protease and amylase in solid granular format due to its
common use in feed applications. These enzymes were subjected to varying conditions by
altering time, temperature, pH, and relative humidity. They were analyzed in regard to their
stability and degradation over time.
The results increased the general knowledge of enzyme degradation caused by the mentioned
parameters, providing the information of different degradation mechanisms at different
humidities and temperatures. The small-scale system proved successful and onwards, larger
stability screenings are possible. The results regarding acceleration of stability studies are
somewhat ambiguous and more data is still needed for clarity. (Less)
Please use this url to cite or link to this publication:
author
Andersson, Erika LU
supervisor
organization
course
KBTM05 20241
year
type
H2 - Master's Degree (Two Years)
subject
keywords
biotechnology, enzyme stability, enzyme degradation, unfolding temperature, enzyme activity
language
English
id
9164697
date added to LUP
2024-06-20 14:54:42
date last changed
2024-06-20 14:54:42
@misc{9164697,
  abstract     = {{Enzyme formulation development is a tedious process that requires lots of time and resources.
By improving parts of the formulation process, the formulation of enzymes could become more
efficient and allow for quicker commercialization of enzyme products. The focus of this project
has been on solid granular enzyme formulations, aiming to accelerate stability studies, develop
enzyme degradation prediction models and validate a small-scale screening system. The three
feed enzymes of phytase, amylase and protease were subjected to varying time, temperature,
pH and humidity to then be analyzed using mainly nanoDSF and activity assays. The results
helped widen the understanding of enzyme degradation caused by these various storage
conditions and prediction of enzyme stability has been validated, while also showing that high
enzyme stability and high unfolding temperatures do not always correlate. The humidity has a
substantial effect on enzyme stability. Also, the possibility of accelerating stability studies
remains uncertain. Lastly, a small scale screening system has been successfully developed.}},
  author       = {{Andersson, Erika}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Stability screening of enzyme formulation in solid format}},
  year         = {{2024}},
}